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    Wyświetlanie 1-3 z 3
    Tytuł:
    The rapid interphase chromosome assay (RICA) implementation : comparison with other PCC methods
    Autorzy:
    Sommer, S.
    Buraczewska, I.
    Sikorska, K.
    Bartłomiejczyk, T.
    Szumiel, I.
    Kruszewski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/147263.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    biodosimetry
    ionizing radiation
    premature chromosome condensation (PCC)
    human lymphocytes
    Opis:
    A report is presented on the advantages of the rapid interphase chromosome assay (RICA) and the difficulties that may be met while implementing this method for application in biological dosimetry. The RICA test can be applied on unstimulated human lymphocytes; this is an advantage in comparison with the dicentric chromosomes or micronucleus tests. In the former two tests, stimulated lymphocytes are examined and hence, 48 h more are needed to obtain cells traversing the cell cycle. Due to the use of unstimulated nondividing cells, higher numbers of cells are available for RICA analysis than for dicentric chromosomes or micronuclei tests. Moreover, the method can be applied after exposure to ionizing radiation doses in excess of 5 Gy. Such doses cause a signifi cant cell cycle delay or result in the loss of G2 phase and mitotic cells because of apoptosis. Therefore, the traditional biodosimetry based on the evaluation of the incidence of damage to chromosomes is very difficult to carry out. This is due to the lack of an adequate number of mitotic cells for analysis. RICA is free of this disadvantage. An automatic microscope can be used to retrieve cell images; automatic image analysis can also be used.
    Źródło:
    Nukleonika; 2015, 60, No. 4, part 2; 933-941
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    DNA damage in subpopulations of human lymphocytes irradiated with doses in the range of 0-1 Gy of X-radiation
    Autorzy:
    Wojewódzka, M.
    Machaj, E. K.
    Goździk, A.
    Iwaneńko, T.
    Ołdak, T.
    Kruszewski, M.
    Pojda, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/147760.pdf
    Data publikacji:
    2008
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    alkaline comet assay
    biodosimetry
    micronucleus test
    histone gammaH2AX foci immunofluorescence assay
    T lymphocytes
    B lymphocytes
    ionizing radiation
    DNA damage
    Opis:
    We compared three methods usually applied in biological dosimetry for estimation of radiation-induced DNA damage in human T and B lymphocytes: alkaline comet assay, micronucleus (MN) test and formation of histone gamma-H2AX foci. Human peripheral blood lymphocytes were fractionated using T cells and B cells isolation kits. Cells were irradiated with doses in the range of 0-1 Gy of X-rays. Induction of DNA damage was assessed by the standard alkaline comet assay, MN test and histone gammaH2AX foci immunofluorescence assay. Notwithstanding different end-points measured by the applied methods, all tests revealed a similar induction of DNA damage in B lymphocytes as compared with T lymphocytes. The results indicated that all three tests detect DNA damage with similar sensitivity, the lowest dose being approximately 0.3 Gy. The difference between irradiated and control cells was expressed as the ratio of the value obtained for irradiated cells (1 Gy) to that for control cells. The highest ratio was obtained for formation of gammaH2AX foci and was 6.2 for T and 13.8 for B lymphocytes, whereas those for comet assay and micronucleus test were 3.5; 3.6 and 5.6; 4.8, respectively.
    Źródło:
    Nukleonika; 2008, 53, 4; 145-149
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Treatment with silver nanoparticles delays repair of X-ray induced DNA damage in HepG2 cells
    Autorzy:
    Wojewódzka, M.
    Lankoff, A.
    Dusińska, M.
    Brunborg, G.
    Czerwińska, J.
    Iwaneńko, T.
    Stępkowski, T.
    Szumiel, I.
    Kruszewski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/147061.pdf
    Data publikacji:
    2011
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    silver nanoparticles (Ag NP)
    titanium dioxide nanoparticles (TiO2 NP)
    DNA break rejoining
    alkaline comet assay
    ionizing radiation
    combined treatment with nanoparticles + X-rays
    Opis:
    Nanoparticles (NPs) defined as particles having at least one dimension below 100 nm have been applied in the last decade in industry and medicine. Recently, there is an increased concern about the biohazard aspect of the presence of NP in consumer goods and in the environment. Silver NP (Ag NP) cause oxidative stress in mammalian cells in result of generation of reactive oxygen species (ROS). This results in genotoxicity and mutagenicity, disturbed mitochondrial respiration, slowed proliferation and cell death. Using the alkaline comet assay, we examined the effect of combined treatment with Ag NP 20 nm or 200 nm and X-rays (2 Gy) in HepG2 cells. In addition, combined treatment with X-rays and titanium dioxide NP (TiO2 NP) 21 nm was also studied. No effect of NP pre-treatment on X-ray induced initial deoxyribonucleic acid (DNA) damage levels was observed for all three NP. In contrast, Ag NP treatment preceding exposure to X-rays caused a marked decrease in the rate of single strand break rejoining. The effect was particularly strong for Ag NP 20 nm. TiO2 NP pre-treatment had no effect on DNA repair.
    Źródło:
    Nukleonika; 2011, 56, 1; 29-33
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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