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Wyszukujesz frazę "Kwiatek, W." wg kryterium: Autor

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    Wyświetlanie 1-4 z 4
    Tytuł:
    Investigation of Trace Element Concentration in Diabetic Rats Tissues
    Autorzy:
    Kowalska, J.
    Krośniak, M.
    Gryboś, R.
    Kwiatek, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/1809705.pdf
    Data publikacji:
    2009-02
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    29.30.Ep
    78.70.En
    87.19.X-
    Opis:
    Diabetes is one of the most frequent diseases in developing countries and thus there is a significant interest in diabetes related studies. It was found that vanadium compounds have glucose-lowering properties in diabetes and therefore it is very important to estimate the vanadium dose in diabetes treatment. On the other hand, the proper estimation of vanadium concentration is important due to side effects that occur in vanadium supplementation. In this study the influence of V(IV) and V(V) compounds with different ligands on the concentration of K, Ca, Mn, Fe, Cu, and Zn in selected rat's tissues was investigated by means of proton induced X-ray emission technique. As a result of the measurements it was found that the concentration of vanadium depends on the organ. The highest value was determined in spleen while the lowest in pancreas. It was also found that the concentration of other elements depends on the presence of vanadium and its concentration. The most meaningful influence of vanadium presence was on iron concentration in spleen, on copper and zinc in kidney, and on manganese in pancreas.
    Źródło:
    Acta Physica Polonica A; 2009, 115, 2; 556-560
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    FTIR Microspectroscopy in Studies of DNA Damage Induced by Proton Microbeam in Single PC-3 Cells
    Autorzy:
    Lipiec, E.
    Kowalska, J.
    Lekki, J.
    Wiecheć, A.
    Kwiatek, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/1490193.pdf
    Data publikacji:
    2012-02
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    87.14.gk
    87.64.km
    78.30.Jw
    87.53.Bn
    Opis:
    In recent years, the Fourier transformed infrared spectroscopy is often applied in studies of biological materials on cellular level. Undoubted advantage of this method is high sensitivity. In presented research the FTIR microspectroscopy was used to analyse the DNA damage in single PC-3 cells (prostate cancer cell line derived from bone metastases) irradiated by counted number of protons. Focused proton microbeam 2 MeV from the Van de Graaff accelerator at the Institute of Nuclear Physics, Polish Academy of Sciences, was used as an irradiation source. Four groups of single cells were irradiated with 1000, 2000, 4000, and 8000 protons per cell, respectively. Following irradiation cells were fixed in 70% ethanol and then analyzed by IR microspectroscopy. Bond analysis of IR spectra served as a base for result analysis. This research has focused on the detection of changes in DNA backbone spectral range (950-1240 $cm^{-1}$), which could be related to damages such as single and double strand breaks, DNA-DNA, and DNA-protein cross links. Switches and differences in intensity of DNA backbone bands (980-1149 $cm^{-1}$, 1151-1350 $cm^{-1}$ - symmetric and asymmetric $PO^{2-}$ stretching vibrations, as well as in 1110 $cm^{-1}$ - symmetric stretching of P-O-C band) were observed. Experimental spectra of irradiated and control cells were compared with simulated spectra generated by HyperChem software. The multivariate statistical methods of principal component analysis and hierarchical cluster analysis (Ward's method) were also performed and are discussed.
    Źródło:
    Acta Physica Polonica A; 2012, 121, 2; 506-509
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Infrared Spectroscopy in Molecular Study of the Piezoelectric Effect in Pigs Shin Bone
    Autorzy:
    Lipiec, E.
    Kowalska, J.
    Wiecheć, A.
    Zieliński, P.
    Kwiatek, W.
    Iwaniec, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/1490248.pdf
    Data publikacji:
    2012-02
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    78.30.Jw
    07.57.Ty
    77.65.-j
    87.50.ct
    Opis:
    Despite growing number of literature demonstrating the piezoelectric effect in bones at macro level there's still a lack of papers describing this effect at molecular level. In order to examine this effect more specifically, a study has been conducted to show the possibility of using infrared spectroscopy on samples contained in the electric field. This is the first known research on applying infrared spectroscopy to study the influence of electric field and compressive stress on bones' structure represented by the molecular IR spectrum. The samples used in this experiment as a model were prepared in pellet form, made from powdered pig's shin bone mixed with KBr (mass ratio of about 1:100). The spectra were obtained in transmission mode. Three different types of experiments were performed at each sample, which were subjected to: (a) fixed electric field (E < 40 kV/m), (b) variable electric field (0-40 kV/m), (c) compressive stress (P < 100 MPa). The obtained data have shown that the electric field and compressive stress on sample modified infrared spectra of the bone. Qualitative as well as quantitative changes in the spectral range between 900 $cm^{-1}$ and 1200 $cm^{-1}$ and the band at 562 $cm^{-1}$ ($PO_4^{3-}$ asymmetric and symmetric stretching, respectively) were observed. The relative area obtained by decomposition of the $PO_4^{3-}$ band reaches an extreme value in the external compressive stress (10 MPa), that occur under physiological conditions. Based on presented data, it was proved that the applied factors (electric field, compressive stress) can change the oscillation energy and the number of molecule's degrees of freedom.
    Źródło:
    Acta Physica Polonica A; 2012, 121, 2; 539-542
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Chemical Composition of Atherosclerotic Plaques οf apoE/LDLR-Double Knockout Mice by Synchrotron Radiation FTIR Microspectroscopy
    Autorzy:
    Kowalska, J.
    Gajda, M.
    Kwiatek, W.
    Franczyk-Żarów, M.
    Kostogrys, R.
    Chłopicki, S.
    Sandt, C.
    Dumas, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/1490259.pdf
    Data publikacji:
    2012-02
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    87.19.xr
    87.64.km
    87.64.-t
    Opis:
    Atherosclerosis is a multietiological inflammatory disease of large and medium-sized arteries of increasing incidence in westernized countries. The aim of this study was to identify the biochemical changes during the progression of atherosclerosis by synchrotron radiation Fourier transform infrared microspectroscopy in atheromas of apoE/$LDLR^{-//-}$ mice fed egg-rich diet supplemented or not with angiotensin converting enzyme inhibitor perindopril. Synchrotron radiation Fourier transform infrared microspectroscopy technique was used to obtain information at high spatial resolution about the distribution of proteins (C-N, N-H, CO for amide I and amide II bands), lipids ($CH_2$, $CH_3$ bands) as well as mineral deposits (calcium carbonates and phosphates). Total contents of lipids and proteins were found to be significantly lower in animals treated with the diet and perindopril. An increase in saturation level of lipids was observed in animals fed with egg-rich diet when compared to the normal diet and perindopril treatment, which did not inhibit this effect. Moreover, a significant change in the secondary structure of proteins (ratio between absorption bands 1634 $cm^{-1}$/1656 $cm^{-1}$ attributed to β-type and α-type, respectively) was observed in both experimental groups in comparison with the control. Principal component analysis was used to analyse the recorded spectra. It has revealed that higher content of phosphates (wavenumber range 950-1020 $cm^{-1}$) was observed between egg-rich diet fed animals and the control group.
    Źródło:
    Acta Physica Polonica A; 2012, 121, 2; 555-560
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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