- Tytuł:
- Utilization of specific primers in legume allergens based polymorphism screening
- Autorzy:
-
Klongová, Lucia
Kováčik, Adam
Urbanová, Lucia
Kyseľ, Matúš
Ivanišová, Eva
Žiarovská, Jana - Powiązania:
- https://bibliotekanauki.pl/articles/2086185.pdf
- Data publikacji:
- 2021
- Wydawca:
- Państwowa Wyższa Szkoła Zawodowa w Tarnowie
- Tematy:
-
legume
profilins
vicilin
specific primer
polymorphism
roślina strączkowa
profiliny
wicylina
starter specyficzny
polimorfizm - Opis:
- Different types of allergies became a part of life of many people around the world. The research activities connecting to allergens are actually not oriented only for protein and immunological interactions, but to the genomic and transcriptomic background of them, too. Analysis and description of genomic variability of allergens in plant food resources will help to manage the allergen based strategies in the future. Here, the bioinformatic approach was used to develop and validate the specific primers for genomic screening of polymorphism of profilins (Profilin Based Amplicon Polymorphism; PBAP) and vicilins (Vicilin Based Amplicon Polymorphism; VBAP) among the legumes. The alignment of existing public databases data for these allergens in the group of legumes was performed. Subsequently, specific primers were designed and their ability to generate polymorphic amplicons were tested for three legumes – bean, lentil and chickpeas. In all cases, amplicons were generated and polymorphism was detected in all three species for profilin as well as for vicilin.
- Źródło:
-
Science, Technology and Innovation; 2021, 13, 2; 12--21
2544-9125 - Pojawia się w:
- Science, Technology and Innovation
- Dostawca treści:
- Biblioteka Nauki
Artykuł