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Wyświetlanie 1-3 z 3
Tytuł:
Microscopic analysis of the nanostructures impact on endothelial cells
Autorzy:
Kołodziejczyk, Agnieszka Maria
Kucińska, Magdalena
Jakubowska, Aleksandra
Siatkowska, Małgorzata
Sokołowska, Paulina
Kotarba, Sylwia
Makowski, Krzysztof
Komorowski, Piotr
Walkowiak, Bogdan
Powiązania:
https://bibliotekanauki.pl/articles/1844983.pdf
Data publikacji:
2020
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
nanostructures
atomic force microscopy
transmission electron microscopy
scanning electron microscopy
cell morphology
Opis:
Nowadays nanostructures are more and more often designed as carriers for drug delivery, especially to improve the drug pharmacokinetics and pharmaco-dynamics. Numerous kinds of nanostructures are considered a good prospect for medical applications thanks to their small size, acceptable biocompatibility and toxicity. Due to the fact that nanotechnology is a new field of science, every nano-scale product must be thoroughly examined regarding its toxicity to the human body. This study provides new insights into effects of exposing endothelial cells to the selected nanostructures. Dendrimers of the fourth generation (PAMAMs), multi-walled carbon nanotubes (MWCNTs) and silver nanoparticles (SNPs) were used to evaluate nanostructures influence on endothelial cells in vitro. The nanostructures were evaluated via transmission electron microscopy and dynamic light scattering technique. The cells previously exposed to the nanostructures were observed and analyzed via the atomic force microscopy and scanning electron microscopy to obtain a quantitative evaluation of the cells morphology. The presence of multi-walled carbon nanotubes and silver nanoparticles on the cells surface was confirmed by the scanning electron microscopy. Our results confirm that the surface association and/or uptake of nanostructures by the cells resulting from physicochemical and biological processes, affect the cells morphology. Morphological changes can be induced by the membrane proteins interaction with nanomaterials, which trigger a sequence of intracel-lular biological processes.
Źródło:
Engineering of Biomaterials; 2020, 23, 154; 2-8
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microscopic analysis of the nanostructures impact on endothelial cells
Autorzy:
Kołodziejczyk, Agnieszka Maria
Kucińska, Magdalena
Jakubowska, Aleksandra
Siatkowska, Małgorzata
Sokołowska, Paulina
Kotarba, Sylwia
Makowski, Krzysztof
Komorowski, Piotr
Walkowiak, Bogdan
Powiązania:
https://bibliotekanauki.pl/articles/970974.pdf
Data publikacji:
2020
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
nanostructures
atomic force microscopy
transmission electron microscopy
scanning electron microscopy
cell morphology
Opis:
Nowadays nanostructures are more and more often designed as carriers for drug delivery, especially to improve the drug pharmacokinetics and pharmaco-dynamics. Numerous kinds of nanostructures are considered a good prospect for medical applications thanks to their small size, acceptable biocompatibility and toxicity. Due to the fact that nanotechnology is a new field of science, every nano-scale product must be thoroughly examined regarding its toxicity to the human body. This study provides new insights into effects of exposing endothelial cells to the selected nanostructures. Dendrimers of the fourth generation (PAMAMs), multi-walled carbon nanotubes (MWCNTs) and silver nanoparticles (SNPs) were used to evaluate nanostructures influence on endothelial cells in vitro. The nanostructures were evaluated via transmission electron microscopy and dynamic light scattering technique. The cells previously exposed to the nanostructures were observed and analyzed via the atomic force microscopy and scanning electron microscopy to obtain a quantitative evaluation of the cells morphology. The presence of multi-walled carbon nanotubes and silver nanoparticles on the cells surface was confirmed by the scanning electron microscopy. Our results confirm that the surface association and/or uptake of nanostructures by the cells resulting from physicochemical and biological processes, affect the cells morphology. Morphological changes can be induced by the membrane proteins interaction with nanomaterials, which trigger a sequence of intracel-lular biological processes.
Źródło:
Engineering of Biomaterials; 2020, 23, 154; 2-8
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In situ-formed bacterial exopolysaccharide (EPS) as a potential carrier for anchorage-dependent cell cultures
Autorzy:
Komorowski, Piotr
Kołodziejczyk, Agnieszka
Makowski, Krzysztof
Kotarba, Sylwia
Walkowiak, Bogdan
Powiązania:
https://bibliotekanauki.pl/articles/1844871.pdf
Data publikacji:
2021
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
bacterial exopolysaccharides
dextran- -based “microcarriers”
scanning electron microscopy
atomic force microscopy
roughness parameters
three-dimensional cell culture
Opis:
The study involved the use of a bacterial strain isolated from environmental samples which produce the biopolymer in the form of pellets in the submerged culture. This material (bacterial exopolysaccharide) is produced by bacteria of the Komogateibacter xylinus which are prevalent in the environment. The aim of this study was to characterize bacterial exopolysaccharides and commercial dextran-based “microcarriers” in terms of their roughness and cell culture effects, including the morphology and viability of the human hybridoma vascular endothelial cell line EA.hy926. The pellets were characterized using scanning electron microscopy (SEM) and atomic for¬ce microscopy (AFM). The resulting structures were used for cell culture of adherent cells (anchorage¬-dependent cells). At the same time, the cultures with commercial, dextran-based “microcarriers” were carried out for comparative purposes. After com¬pletion of the cell culture (24 hours of culture), the cellulose and commercial “carriers” were analyzed using SEM and AFM. Finally, the obtained cell dens¬ities (fluorescence labelling) and their morphological characteristics (SEM) were compared. The obtained results strongly support the applicability of bacterial exopolysaccharide (EPS) in tissue engineering to build innovative 3D scaffolds for cell culture, the more so that it is technologically possible to produce EPS as spatially complex structure
Źródło:
Engineering of Biomaterials; 2021, 24, 159; 18-23
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-3 z 3

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