- Tytuł:
- Limitation of usage of PicoGreen dye in quantitative assays of double-stranded DNA in the presence of intercalating compounds
- Autorzy:
-
Koba, Marcin
Szostek, Alicja
Konopa, Jerzy - Powiązania:
- https://bibliotekanauki.pl/articles/1040892.pdf
- Data publikacji:
- 2007
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
quantitative assay
interstrand DNA cross-link determination
PicoGreen dye
double-stranded DNA
drug-DNA binding - Opis:
- PicoGreen is a very sensitive fluorescent dye for quantitative assays of double-stranded DNA (dsDNA) in solution and is used in several analytical protocols in which sensitive and precise DNA detection is needed, also for examination of drug-DNA interactions. The data shown in this paper indicate that compounds intercalating to DNA influence the applicability of PicoGreen dye for quantitative measurements of dsDNA, and for this reason PicoGreen dye is not suitable for examination of drug-DNA interactions, especially interstrand DNA crosslinks.
- Źródło:
-
Acta Biochimica Polonica; 2007, 54, 4; 883-886
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł