Autor: Bochniarz, M - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Concentration of serum amyloid A and ceruloplasmin activity in milk from cows with subclinical mastitis caused by different pathogens
Autorzy:: Szczubial, M.
Dabrowski, R.
Kankofer, M.
Bochniarz, M.
Komar, M.
Powiązania:: https://bibliotekanauki.pl/articles/31564.pdf
Data publikacji:: 2012
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:: The aim of the present study was to determine the concentration of serum amyloid A (SAA) and the activity of ceruloplasmin (Cp) in milk from cows with subclinical mastitis caused by different pathogens. Eighty-four milk samples from cows with subclinical mastitis and fourteen milk samples from healthy cows were examined. SAA concentration was determined using the commercial ELISA kit (Tridelta Development Ltd., Greystones, Wicklow, Ireland). Cp activity was assessed spectrophotometrically, using the Rice method. The results reveal that the concentration of SAA (with exception of CNS) and activity of Cp in cow milk can be regarded as markers of subclinical mastitis, irrespective of the microorganism inducing the disease. In conclusion, measurement of SAA and Cp in milk samples could be a useful method in diagnosing subclinical mastitis in cows, but the method should be adapted for field use.
Źródło:: Polish Journal of Veterinary Sciences; 2012, 15, 2
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Assessment of lipid and protein peroxidation markers in non-pregnant and pregnant female dogs
Autorzy:: Szczubial, M.
Kankofer, M.
Dabrowski, R.
Bochniarz, M.
Urban-Chmiel, R.
Powiązania:: https://bibliotekanauki.pl/articles/2087973.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:: The aim of the study was to investigate oxidative stress during normal pregnancy in female dogs based on an evaluation of plasma markers for lipid and protein peroxidation. Twenty clinically healthy female dogs (10 non-pregnant and 10 pregnant) were used in the study. Blood samples from the pregnant animals were collected at 19-21, 38-40, and 56-58 days of pregnancy. Blood samples from non-pregnant female dogs were obtained between 20 and 35 days after ineffective breeding. As indicators of oxidative stress, we measured the following using spectrophotometric and spectrof-luorimetric methods: thiobarbituric acid reactive substances (TBARS), radical cations of N,N, die-thylpara-phenylene diamine (RC-DEPPD), sulfhydryl groups (SH groups), bityrosine and formyl-kynurenine. The mean plasma TBARS concentration in the pregnant dogs (0.486 ± 0.071 - 0.581 ± 0.191 |imol/g protein) was significantly higher (p<0.05) than that found in the non-pregnant animals (0.274 ± 0.111 |imol/g protein). A marked, although not significant, decrease in SH group content, as well as an increase in bityrosine and formylkynurenine concentration were concurrently observed in the pregnant dogs. No significant differences were found in terms of the studied markers in the pregnant animals when comparing the values obtained during the investigated periods of pregnancy, although there was a progressive decrease in TBARS concentration and a progressive increase in RC-DEPPD, bityrosine and formylkynurenine contents. Our findings suggest that normal pregnancy in female dogs is associated with oxidative stress. Further studies are necessary to establish the physiological ranges of antioxidative/oxidative profiles in pregnant dogs and to explain if and how the intensity of oxidative stress might contribute to the risk of the complications of pregnancy.
Źródło:: Polish Journal of Veterinary Sciences; 2015, 18, 1
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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