- Tytuł:
- Improved HPLC method for total plasma homocysteine detection and quantification
- Autorzy:
-
Sawuła, Wojciech
Banecka-Majkutewicz, Zyta
Kadziński, Leszek
Jakóbkiewicz-Banecka, Joanna
Węgrzyn, Grzegorz
Nyka, Walenty
Banecki, Bogdan - Powiązania:
- https://bibliotekanauki.pl/articles/1040824.pdf
- Data publikacji:
- 2008
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
homocysteine measurement
stroke risk factors
HPLC
validation - Opis:
- Recent clinical research has pointed at hyperhomocysteinemia as an independent risk factor in a number of cardiovascular and neurological diseases. We have improved a chromatographic method of total plasma homocysteine measurements in order to obtain higher sensitivity, reliability and reproducibility. The method demonstrates excellent linearity (R = 0.999), range (< 2-100 µM), precision (instrumental RSD 0.06 and method RSD 1.17), accuracy (recovery of 99.92 and RSD 1.27), reproducibility, quantification limit and ruggedness (e.g. pH from 2.0 to 2.5). Because even a small increase in homocysteine level can be a significant risk factor of cardiovascular diseases, such a precise method is required. The constructed method allows the measurement of plasma pyridoxal phosphate, PLP, the co-enzyme form of vitamin B6, on the same column and similar reagents. The developed method has been successfully applied to measure both total plasma and serum homocysteine in a group of acute stroke patients.
- Źródło:
-
Acta Biochimica Polonica; 2008, 55, 1; 119-126
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł