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    Wyświetlanie 1-3 z 3
    Tytuł:
    CO2 concentration affects in vitro pig embryo developmental capacity
    Autorzy:
    Zhang, L.
    Lin, Z.
    Bi, Y.
    Zheng, X.
    Xiao, H.
    Hua, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087657.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    CO2
    developmental capacity
    pig embryos
    in vitro
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 3; 609-614
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Impact of different sources of donor cells upon the nuclear transfer efficiency in Chinese indigenous Meishan pig
    Autorzy:
    Hua, Z.
    Xu, G.
    Liu, X.
    Bi, Y.
    Xiao, H.
    Hua, W.
    Li, L.
    Zhang, L.
    Ren, H.
    Zheng, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/31224.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Somatic cell nuclear transfer (SCNT) is currently the most efficient and precise method to generate genetically tailored pig models for both agricultural and biomedical research. However, its efficiency is crucially dependent on the source of nuclear donor cells. In this study, we compared the cloning efficiency by using three lines of donor cells that are derived from fetal, newborn and adult fibroblasts of Chinese indigenous Meishan pig. We showed that cleavage rate and blastocyst formation rate of the reconstructed embryos were not significantly different between the fetal (80.7% and 15.6%) and newborn ear skin (77.5% and 12.3%) fibroblast groups (p>0.05), but in both groups these indices were significantly higher than that found in the adult ear skin (70.5% and 8.8%; p<0.05). Reconstructed embryos derived from fetal, newborn, and adult ear skin fibroblasts were transferred to four surrogates, respectively. For the fetal, newborn, and adult ear skin fibroblasts, the number of pregnancies were two (50.0%), two (50.0%), and one (25.0%), respectively, and the number of deliveries were two (50.0%), one (25.0%), and zero (0.0%), respectively. Seven and two cloned piglets were obtained from the fetal and newborn ear skin fibroblasts respectively, while no piglets were obtained from the adult ear skin fibroblasts. Two cloned piglets from the newborn ear skin fibroblasts died shortly after birth because of neonatal asphyxia caused by dystocia. The birth weights of the piglets derived from the fetal and newborn ear skin fibroblasts were 1230.5 and 1310.0g, respectively, which were statistically insignificant (p>0.05), but both were significantly higher than that of the control groups (p<0.05). Microsatellite analyses demonstrated that the genotypes of all cloned piglets were identical to their donor cells. Therefore, cloned pigs were successfully produced using two sources of donor cells isolated from the fetal and newborn ear skin fibroblasts of Meishan piglet, and indicating a better cloning efficiency than that obtained from adult fibroblasts. We concluded that the nuclear donor cell lines have significant impact on the developmental competence of cloned embryos as well as on the cloning efficiency of Meishan pig.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A new adaptation for in vitro co-culture of single porcine parthenogenetic embryos using feeder cells
    Autorzy:
    Zhang, L.
    Lin, Z.
    Hua, Z.
    Zheng, X.
    Xiao, H.
    Hua, W.
    Ren, H.
    Zhu, Z.
    Molenaar, A.
    Bi, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087368.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    feeder cells
    porcine
    embryos
    in vitro
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 4; 711-716
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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