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    Wyświetlanie 1-6 z 6
    Tytuł:
    Development of an indirect enzyme-linked immunosorbent assay for the detection of novel chicken orthoreovirus
    Autorzy:
    Wei, Z.
    Yang, J.
    Hu, J.
    Tang, Y.
    Diao, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/16539093.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    novel avian orthoreovirus
    σC gene
    recombinant plasmid
    indirect enzyme linked immunosorbent assay
    epidemiological investigation
    Opis:
    A novel avian orthoreovirus (N-ARV) variant characterized with obvious arthritis and synovial inflammation, was isolated from Shandong, China in May 2016. It caused chicken poor growth and enormous economic losses to the poultry industry of China. However, there are few effective methods for detecting the antibody levels of N-ARV. In this study, a viral structural protein σC was expressed using the prokaryotic expression vector pET32a (+). The target protein was obtained by inducing for 6 hours at an IPTG concentration of 0.6mM. The optimal dilution of the coating antigen and serum antibody were determined to be 1000 fold and 10 fold respectively. A specificity test showed that there was no positive reactivity between N-ARV and other pathogens, and when the positive serum was diluted 100 times detection results were still checkable. The repeatability of this method was determined by the inter assay and intra assay tests with variability ranging from 4.85% to 7.93%. In conclusion, this indirect enzyme linked immunosorbent assay (ELISA) will be useful for large-scale serological surveys and monitoring antibody levels in N-ARV infection.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 1; 109-118
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The expression profile of miR-222b-5p/MAPK10 in spleens of SPF chickens infected with REV-SNV at 28-42 dpi
    Autorzy:
    Jiang, H.
    Gao, S.
    Mao, M.
    Diao, Y.
    Tang, Y.
    Hu, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087117.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    reticuloendotheliosis virus strain SNV
    RLRs signaling pathway
    gga-miR-222b-5p
    mitogen-activated protein kinase 10
    a dual-luciferase reporter gene experiment
    Źródło:
    Polish Journal of Veterinary Sciences; 2021, 24, 3; 439-443
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Water-exit process modeling and added-mass calculation of the submarine-launched missile
    Autorzy:
    Yang, J.
    Feng, J.
    Li, Y.
    Liu, A.
    Hu, J.
    Ma, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/260251.pdf
    Data publikacji:
    2017
    Wydawca:
    Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
    Tematy:
    submarine-launched missile
    fluid-structure interaction
    water-exit dynamic model
    time-varying added-mass
    numerical simulation
    Opis:
    In the process that the submarine-launched missile exits the water, there is the complex fluid solid coupling phenomenon. Therefore, it is difficult to establish the accurate water-exit dynamic model. In the paper, according to the characteristics of the water-exit motion, based on the traditional method of added mass, considering the added mass changing rate, the water-exit dynamic model is established. And with help of the CFX fluid simulation software, a new calculation method of the added mass that is suit for submarine-launched missile is proposed, which can effectively solve the problem of fluid solid coupling in modeling process. Then by the new calculation method, the change law of the added mass in water-exit process of the missile is obtained. In simulated analysis, for the water-exit process of the missile, by comparing the results of the numerical simulation and the calculation of theoretical model, the effectiveness of the new added mass calculation method and the accuracy of the water-exit dynamic model that considers the added mass changing rate are verified.
    Źródło:
    Polish Maritime Research; 2017, S 3; 152-164
    1233-2585
    Pojawia się w:
    Polish Maritime Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A Simplified Approach for the Hydro-forming Process of Bi-metallic Composite Pipe
    Autorzy:
    Zheng, M.
    Gao, H.
    Teng, H.
    Hu, J.
    Tian, Z.
    Zhao, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/355930.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    metallic pipe
    composite
    hydroforming
    pressure
    residual stress
    Opis:
    In this article, it aims to propose effective approaches for hydro-forming process of bi-metallic composite pipe by assuming plane strain and elastic-perfectly plastic material model. It derives expressions for predicting hydro-forming pressure and residual stress of the forming process of bi-metallic composite pipe. Test data from available experiments is employed to check the model and formulas. It shows the reliability of the proposed model and formulas impersonally.
    Źródło:
    Archives of Metallurgy and Materials; 2017, 62, 2A; 879-883
    1733-3490
    Pojawia się w:
    Archives of Metallurgy and Materials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Internal Friction of Li7La3Zr2O12 Based Lithium Ionic Conductors
    Autorzy:
    Wang, X. P.
    Song, L.
    Hu, J.
    Xia, Y. P.
    Xia, Y.
    Gao, Y. X.
    Zhang, L. C.
    Magalas, L. B.
    Fang, Q. F.
    Powiązania:
    https://bibliotekanauki.pl/articles/958222.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    lithium ionic conductor
    Li7La3Zr2O12
    internal friction
    Opis:
    The diffusion mechanisms of lithium ions in tetragonal phase as well as in Al and Nb stabilized cubic Li7La3Zr2O12 compounds were investigated by low-frequency internal friction technique. In the cubic Li7La3Zr2O12 phase, a remarkable relaxation-type internal friction peak PC with a peak height up to 0.12 was observed in the temperature range from 15°C to 60°C. In the tetragonal phase however, the height of the PT peak dropped to 0.01. The obvious difference of the relaxation strength between the cubic and tetragonal phases is due to the different distribution of lithium ions in lattice, ordered in the tetragonal phase and disordered in the cubic phase. Based on the crystalline structure of the cubic garnet-type Li7La3Zr2O12 compound, it is suggested that the high internal friction peak in the cubic phase may be attributed to two diffusion processes of lithium ions: 96h↔96h and 96h↔24d.
    Źródło:
    Archives of Metallurgy and Materials; 2016, 61, 1; 21-24
    1733-3490
    Pojawia się w:
    Archives of Metallurgy and Materials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Stability control of propeller Autonomous Underwater Vehicle based on combined sections method
    Autorzy:
    Qi, D.
    Feng, J.
    Liu, A.
    Hu, J.
    Xu, H.
    Li, Y.
    Ashraf, M. A.
    Powiązania:
    https://bibliotekanauki.pl/articles/259119.pdf
    Data publikacji:
    2015
    Wydawca:
    Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
    Tematy:
    propeller AUV
    dynamic model
    stability control
    simulation
    Opis:
    Learning from the motion principle of quadrotor, a symmetric propeller AUV, which has small size and low velocity is designed. Compared with the AUV equipped with rudders, it has better maneuverability and manipulation at low velocity. According to the Newton-Euler method, the 6 DOF kinematic model and dynamic model of the propeller AUV are established. A stability controller that consists of 3 different PID controllers is designed. It makes the depth and attitude angle as trigger conditions, and the relevant controller is chosen in different moving process. The simulation experiments simulate ideal motion state and disturbed motion state, and experiments results show that the stability controller based on combined sections method can make the best of mature technology of PID, and meet the control requirements in different stages. It has a higher respond speed and accuracy, improving the stability of the propeller AUV under the disturbance of complex ocean currents.
    Źródło:
    Polish Maritime Research; 2015, S 1; 157-162
    1233-2585
    Pojawia się w:
    Polish Maritime Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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