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    Wyświetlanie 1-4 z 4
    Tytuł:
    Hydrogen sulfide is involved in the chilling stress response in Vitis vinifera L.
    Autorzy:
    Fu, P.
    Wang, W.
    Hou, L.
    Liu, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/56519.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    hydrogen sulphide
    chilling stress
    stress response
    Vitis vinifera
    Opis:
    Hydrogen sulfide (H2S) is an important signaling molecule involved in several stress-resistance processes in plants, such as drought and heavy metal stresses. However, little is known about the roles of H2S in responses to chilling stress. In this paper, we demonstrated that chilling stress enhance the H2S levels, the H2S synthetase (L-/D-cysteine desulfhydrase, L/DCD) activities, and the expression of L/DCD gene in Vitis vinifera L. ‘F-242’. Furthermore, the seedlings were treated with sodium hydrosulfide (NaHS, a H2S donor) and hypotaurine (HT, a H2S scavenger) at 4°C to examine the effects of exogenous H2S on grape. The results revealed that the high activity of superoxide dismutase and enhanced expression of VvICE1 and VvCBF3 genes, but low level of superoxide anion radical, malondialdehyde content and cell membrane permeability were detected after addition of NaHS. In contrast, HT treatment displayed contrary effect under the chilling temperature. Taken together, these data suggested that H2S might be directly involved in the cold signal transduction pathway of grape.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2013, 82, 4
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Expression and characterisation of cucumber fruit flesh thickness-related gene CSA2M058670.1
    Autorzy:
    Wang, W.
    Wang, S.
    Guo, Y.
    Li, M.
    Hou, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/12709293.pdf
    Data publikacji:
    2020
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Opis:
    Bulked segregant analysis combined with specific length amplified fragment sequencing techniques have been applied to determine the fine genetic mapping of fruit flesh thickness-related genes in cucumber. Herein, the Csa2M058670.1 gene was subjected to real time fluorescence quantitative PCR (qRT-PCR) and sequence analysis, indicating a strong correlation with cucumber fruit flesh thickness. Expression and characterisation of the Csa2M058670.1 gene were performed based on previous studies. The results of the fluorescence-based quantitative PCR showed that Csa2M058670.1 was expressed in all organs, but levels were highest in fruit peel, fruit flesh, and female flowers. Furthermore, Csa2M058670.1 expression was induced by abiotic stresses including drought, low temperature, and high salt. Domain analysis revealed that the protein encoded by Csa2M058670.1 possesses an SET (Su(var), Enhancern of zeste CE(z), and Trithorax) domain that may control cell division and differentiation. Therefore, we speculated that Csa2M058670.1 might affect fruit flesh thickness in cucumbers by influencing cell division.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2020, 19, 2; 25-33
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Application of in vitro culture techniques to barley [Hordeum vulgare L.] improvement
    Autorzy:
    Ullrich, S E
    Kleinhofs, A.
    Hou, L.
    Jones, B.L.
    Powiązania:
    https://bibliotekanauki.pl/articles/2044453.pdf
    Data publikacji:
    1998
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    tissue culture
    doubled haploid
    mutagenesis
    breeding programme
    anther culture
    in vitro
    callus culture
    somaclonal variation
    barley
    mutation
    Hordeum vulgare
    embryo
    molecular biology
    Opis:
    Several aspects of in vitro culture have potential for cereal improvement. This paper focuses on evaluation of somaclonal variation (SV) from immature embryo callus culture, and doubled haploid (DH) production via anther culture in barley. Genetically stable SV was observed for several seedling morphological traits such as albino, yellow, light green and lethal. SV occurred at approximately half the frequency of azide-induced mutagenesis. The potential for widespread application of anther culture-mediated DH production in barley breeding and genetic studies was increased through culture procedure improvements and understanding the inheritance of anther culture response. Methodology improvements included substitution of inexpensive gelrite for expensive ficoll or agarose, ability to grow anther donor plants under field as well as growth chamber conditions and flexibility in cold pretreatment/storage of anther donor spikes for 4-6 weeks prior to anther plating. From diallel analysis, inheritance of anther culture response was complex with additive and dominance effects for embryoid formation, total plant regeneration and green plant regeneration and reciprocal effects (maternal) for green plant regeneration. High x low responder crosses generated F₁’s that were intermediate in response and low x low crosses sometimes produced F₁ heterosis for green plant regeneration. Therefore, some recalcitrant types appear to be usable in anther culture DH production systems within a breeding program.
    Źródło:
    Journal of Applied Genetics; 1998, 39, 1; 49-58
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    VvWRKY13 enhances ABA biosynthesis in Vitis vinifera
    Autorzy:
    Hao, J.
    Ma, Q.
    Hou, L.
    Zhao, F.
    Liu, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/58583.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Opis:
    Abscisic acid (ABA) plays critical roles in plant growth and development as well as in plants’ responses to abiotic stresses. We previously isolated VvWRKY13, a novel transcription factor, from Vitis vinifera (grapevine), and here we present evidence that VvWRKY13 may regulate ABA biosynthesis in plants. When VvWRKY13 was ectopically expressed in Arabidopsis, the transgenic lines showed delayed seed germination, smaller stomatal aperture size, and several other phenotypic changes, indicating elevated ABA levels in these plants. Sequence analysis of several genes that are involved in grapevine ABA synthetic pathway identified WRKY-specific binding elements (W-box or W-like box) in the promoter regions. Indeed, transient overexpression of VvWRKY13 in grapevine leaves significantly increased the transcript levels of ABA synthetic pathway genes. Taken together, we conclude that VvWRKY13 may promote ABA production by activating genes in the ABA synthetic pathway.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2017, 86, 2
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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