- Tytuł:
- Rehydration of CTMA Modified DNA Powders Observed by NMR
- Autorzy:
-
Harańczyk, H.
Kobierski, J.
Zalitacz, D.
Nowak, P.
Romanowicz, A.
Marzec, M.
Nizioł, J.
Hebda, E.
Pielichowski, J. - Powiązania:
- https://bibliotekanauki.pl/articles/1490149.pdf
- Data publikacji:
- 2012-02
- Wydawca:
- Polska Akademia Nauk. Instytut Fizyki PAN
- Tematy:
- 82.56.Na
- Opis:
- The rehydration of salmon sperm deoxyribonucleic acid (DNA) and cetyltrimethylammonium chloride $(C_{19}H_{42}ClN)$ complexes was observed using hydration kinetics, sorption isotherm, and high power proton relaxometry (at 30 MHz). The hydration kinetics shows (i) a very tightly bound water not removed by incubation over silica gel ($A_0^{h}$ = 0.061 ± 0.004), (ii) a tightly bound water saturating at $A_1^{h}$ = 0.039 ± 0.011, with the hydration time $t_1^{h}$ = (1.04 ± 0.21) h, a loosely bound water fraction (iii) with the hydration time $t_2^{h}$ = (19.1 ± 3.2) h and the contribution progressively increasing with the air humidity. For the hydration at $p//p_0$ = 100%, after $t_0$ = (152.6 ± 2.5) h of incubation the swelling process begins. The swelling time was $t_3^{h}$ = (12.5 ± 5.4) h, and the swelling amplitude $A_3^{h}$ = 0.140 ± 0.016. The sorption isotherm is sigmoidal in form and is fitted by the Dent model with the mass of water saturating primary binding sites Δ M/$m_0$ = 0.102 ± 0.021. Proton free induction decay is a superposition of the immobilized proton signal (Gaussian, with $T_{2S}$* ≈ 30 μs) and two liquid signal components coming from tightly bound ($T_{2 L_1}$* ≈ 100 μs) and loosely bound water fraction with the amplitude proportional to the mass of water added ($T_{2 L_2}$* ≈ 1000 μs).
- Źródło:
-
Acta Physica Polonica A; 2012, 121, 2; 485-490
0587-4246
1898-794X - Pojawia się w:
- Acta Physica Polonica A
- Dostawca treści:
- Biblioteka Nauki
Artykuł