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Wyświetlanie 1-4 z 4
Tytuł:
Two-stage bacteriophage production process supported by the electromagnetic field
Autorzy:
Rakoczy, Rafał
Konopacki, Maciej
Kordas, Marian
Grygorcewicz, Bartłomiej
Powiązania:
https://bibliotekanauki.pl/articles/2202884.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bioreactor
biotechnology
bacteriophage production
electromagnetic field
bioreaktor
biotechnologia
produkcja bakteriofagów
pole elektromagnetyczne
Opis:
The practical applications of bacteriophages are associated with the problems related to the intensification, optimization of process production of this biomaterial and the search for new methods of production. The production of bacteriophages requires a fine balance between the dynamic growth of the bacteriophage and the host. The electromagnetic field (EMF) is a promising biotechnological method for the process production of bacteriophages. This study evaluates the use of various types of EMF to enhance the process. It was found that the process production of bacteriophages is divided into two stages. In the first stage, the influence of various types of EMF on the proliferation process of bacteria (host) was analyzed. Secondly, the process production of bacteriophage was implemented for the optimal infection conditions under the action of the various types of EMF. Moreover, the study demonstrated that the most effective bacteriophage production was the process with the application of the rotating magnetic field (RMF), pulsed magnetic field (PMF) and the static magnetic field (SMF) with negative polarity.
Źródło:
Chemical and Process Engineering; 2022, 43, 4; 491--506
0208-6425
2300-1925
Pojawia się w:
Chemical and Process Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Growth optimization of Klebsiella pneumoniae in magnetically assisted bioreactor
Autorzy:
Konopacki, Maciej
Augustyniak, Adrian
Grygorcewicz, Bartłomiej
Dołęgowska, Barbara
Kordas, Marian
Rakoczy, Rafał
Powiązania:
https://bibliotekanauki.pl/articles/2202874.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bacteria cultivation
growth kinetics
optimization process
magnetically assisted bioreactor
hodowla bakterii
kinetyka wzrostu
proces optymalizacji
bioreaktor wspomagany magnetycznie
Opis:
In recent years, infections are more often caused by pathogens with high multi-drug resistance, classified as the “ESKAPE” microorganisms. Therefore, investigation of these pathogens, e.g., Klebsiella pneumoniae, often requires biomass production for treatment testing such as antibiotics or bacteriophages. Moreover, K. pneumoniae can be successfully applied as a biocatalyst for other industrial applications, increasing the need for this bacteria biomass. In the current study, we proposed a novel magnetically assisted bioreactor for the cultivation of K. pneumoniae cells in the presence of an external alternating magnetic field (AMF). High efficiency of the production requires optimal bacteria growth conditions, e.g., temperature and field frequency. Therefore, we performed an optimization procedure using a central composite design for these two parameters in a wide range. As an objective function, we utilized a novel, previously described growth factor that considers both biomass and bacteria growth kinetics. Thus, based on the response surface, we could specify the optimal growth conditions. Moreover, we analysed the impact of the AMF on bacteria proliferation, which indicated positive field frequency windows, where the highest stimulatory effect of AMF on bacteria proliferation occurred. Obtained results proved that the magnetically assisted bioreactor could be successfully employed for K. pneumoniae cultivation.
Źródło:
Chemical and Process Engineering; 2022, 43, 3; 289--304
0208-6425
2300-1925
Pojawia się w:
Chemical and Process Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dynamic modelling of bacteriophage production process
Autorzy:
Konopacki, Maciej
Grygorcewicz, Bartłomiej
Gliźniewicz, Marta
Miłek, Dominika
Kordas, Marian
Rakoczy, Rafał
Powiązania:
https://bibliotekanauki.pl/articles/2202885.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bacteriophages
mathematical modelling
dynamic modelling
bioprocess engineering
Simulink
bakteriofagi
modelowanie matematyczne
modelowanie dynamiczne
inżynieria bioprocesowa
Opis:
Bacteriophages, viruses that can infect bacteria, are promising alternatives for antibiotic treatment caused by antibiotic-resistant bacteria strains. For that reason, the production of bacteriophages is extensively studied. Mathematical modelling can lead to the improvement of bioprocess by identification of critical process parameters and their impact on the demanded product. Dynamic modelling considers a system (i.e. bioreactor or bioprocess) as a dynamic object focusing on changes in the initial and final parameters (such as biomass concentration and product formation) in time, so-called signals and treats the studied system as a “black box” that processes signals. This work aimed to develop a mathematical model that describes bacteriophage production process. As result, we created a dynamic model that can estimate the number of bacteriophages released from cells as plaque-forming units at specific time points based on the changes in the bacteria host-cell concentration. Moreover, the proposed model allowed us to analyze the impact of the initial virus concentration given by multiplicity of infection (MOI) on the amount of produced bacteriophages.
Źródło:
Chemical and Process Engineering; 2022, 43, 4; 471--482
0208-6425
2300-1925
Pojawia się w:
Chemical and Process Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Studies of a mixing process induced by a rotating magnetic field with the application of magnetic particles
Autorzy:
Rakoczy, Rafał
Kordas, Marian
Markowska-Szczupak, Agata
Konopacki, Maciej
Augustyniak, Adrian
Jabłońska, Joanna
Paszkiewicz, Oliwia
Dubrowska, Kamila
Story, Grzegorz
Story, Anna
Ziętarska, Katarzyna
Sołoducha, Dawid
Borowski, Tomasz
Roszak, Marta
Grygorcewicz, Bartłomiej
Dołęgowska, Barbara
Powiązania:
https://bibliotekanauki.pl/articles/2086782.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
mixing process
alternating magnetic field
magnetic particles
mixing time
Navier–Stokes equation
proces mieszania
zmienne pole magnetyczne
cząstki magnetyczne
czas mieszania
równanie Naviera-Stokesa
Opis:
We demonstrate in this study that a rotating magnetic field (RMF) and spinning magnetic particles using this kind of magnetic field give rise to a motion mechanism capable of triggering mixing effect in liquids. In this experimental work two mixing mechanisms were used, magnetohydrodynamics due to the Lorentz force and mixing due to magnetic particles under the action of RMF, acted upon by the Kelvin force. To evidence these mechanisms, we report mixing time measured during the neutralization process (weak acid-strong base) under the action of RMF with and without magnetic particles. The efficiency of the mixing process was enhanced by a maximum of 6.5% and 12.8% owing to the application of RMF and the synergistic effect of magnetic field and magnetic particles, respectively
Źródło:
Chemical and Process Engineering; 2021, 42, 2; 157--172
0208-6425
2300-1925
Pojawia się w:
Chemical and Process Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-4 z 4

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