- Tytuł:
- Application of electrophoretic methods for detection of protein-porphyrin complexes.
- Autorzy:
-
Kowalska, Agnieszka
Kwiek, Piotr
Miłosz, Ewa
Gondek, Grzegorz
Romiszewska, Anna
Graczyk, Alfreda
Podhajska, Anna - Powiązania:
- https://bibliotekanauki.pl/articles/1043407.pdf
- Data publikacji:
- 2003
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
porphyrins
photodynamic therapy
PDT
electrophoresis - Opis:
- Simple methods for detection and isolation of protein-porphyrin complexes were elaborated in our laboratory. They are based on the separation of protein-porphyrin complexes in native polyacrylamide gel and measurement of their fluorescence, with the use of two detection systems: the commercially available Gel DocTM 2000 system, and a system specially designed for the purpose of these investigations, concerning protein-porphyrin interactions. The fluorescent complexes can be electro-transferred from the gel onto PVDF membrane, eluted and analyzed in order to identify the protein interacting with porphyrins.
- Źródło:
-
Acta Biochimica Polonica; 2003, 50, 4; 1155-1163
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł