- Tytuł:
- A simple method for the determination of the cholesterol esterase activity
- Autorzy:
-
Stępień, Agnieszka
Gonchar, Mykhailo - Powiązania:
- https://bibliotekanauki.pl/articles/1039538.pdf
- Data publikacji:
- 2013
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
cholesterol esterase
cholesterol
activity determination
human serum - Opis:
- The proposed method determines the activity of cholesterol esterase (CEH) and takes advantage of its ability to catalyze the hydrolysis of cholesterol esters naturally present in human serum. The assay is based on Allain's method of spectrophotometric determination of cholesterol by means of cholesterol oxidase, peroxidase, but using 3,5-dichloro-dihydroxybenzenesulfonic acid (DHBS) as phenolic chromogen and human serum as a source of substrate for the CEH as a novelty. Furthermore, it is characterized by low costs and high precision. It can be employed to control the activity of CE preparations used for the preparation of enzymatic kits for the determination of cholesterol or for screening of potential bacterial enzyme producers.
- Źródło:
-
Acta Biochimica Polonica; 2013, 60, 3; 401-403
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł