Temat: biosensor - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Low bod determination methods: the state-of-the-art
Autorzy:: Górski, Ł.
Trzebuniak, K.F.
Malinowska, E.
Powiązania:: https://bibliotekanauki.pl/articles/185812.pdf
Data publikacji:: 2012
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: biochemical oxygen demand
biosensor
wastewater
microbial sensor
biochemiczne zapotrzebowanie na tlen
ścieki
czujnik drobnoustrojów
Opis:: Biochemical Oxygen Demand (BOD) is an important factor used to measure water pollution. This article reviews recent developments of microbial biosensors with respect to their applications for low BOD estimation. Four main methods to measure BOD using a biosensor are described: microbial fuel cells, optical methods, oxygen electrode based methods and mediator-based methods. Each of them is based on different principles, thus a different approach is required to improve the limit of detection. A proper choice of microorganisms used in the biosensor construction and/or sample pre-treatment processes is also essential to improve the BOD lower detection limit.
Źródło:: Chemical and Process Engineering; 2012, 33, 4; 629-637
0208-6425
2300-1925
Pojawia się w:: Chemical and Process Engineering
Dostawca treści:: Biblioteka Nauki

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Tytuł:: Oligonukleotydy DNA jako warstwy receptorowe sensorów elektrochemicznych
DNA oligonucleotides as receptor layers of electrochemical sensors
Autorzy:: Górski, Ł.
Ziółkowski, R.
Bala, A.
Jarczewska, M.
Malinowska, E.
Powiązania:: https://bibliotekanauki.pl/articles/171556.pdf
Data publikacji:: 2015
Wydawca:: Polskie Towarzystwo Chemiczne
Tematy:: biosensor
metale ciężkie
kwasy nukleinowe
samoorganizujące się monowarstwy
woltamperometria
znaczniki redoks
biosensors
heavy metals
nucleic acids
self-assembled monolayers
voltammetry
redox indicators
Opis:: The need for elaboration of analytical devices of small dimensions and the accessibility of novel nanomaterials caused the increase in the number of publications referring to the development of biosensors. DNA-based biosensors are of special interest and they were primarily used for the determination of a specific sequence which is crucial in the detection of cancer, genetic mutations, pathogens, as well as analysis of modified food. Interestingly, they could be also applied for the detection of other analytes including heavy metal ions, especially in connection with electrochemical techniques. It should be noted that the design of DNA biosensor concerns not only the development of transducer, but also careful preparation of sensing layer and the choice of the method of analytical signal generation. Selectivity is one of the essential parameter of the biosensor that determines its utility, particularly in real samples of complex matrices. In case of DNA sensors dedicated for the detection of complementary sequence, high selectivity is provided by the hybridization process. A pronounced specificity of sensing layer-analyte interaction can be also achieved with the use of functional nucleic acids - aptamers, which change their conformation upon binding an analyte. Herein, DNA-modified electrodes were firstly used for the detection of uranyl ions, as they exhibit high affinity towards phosphate moieties of nuclec acids. It was shown that UO₂ ²⁺ interacts with sensing layer independently from the chosen oligonucleotide sequence. Moreover, the influence of Pb²⁺ was reduced by elimination of adenine, which strongly interacts with lead ions. Another oligonucleotide-based sensor was developed for detection of mercury ions. The results indicate that Hg2+ concentration can be determined only with the use of sequence containing 100% thymine residues. Oligonucleotide-based sensor with receptor layer containing aptamers was elaborated for the detection of Pb²⁺ ions. In the presence of lead cations, an aptamer probe forms a G-quadruplex structure, a proposed biosensor could be characterized with selectivity towards Pb²⁺ The performance of DNA-based sensors for UO₂ ²⁺, Hg²⁺ and Pb²⁺ ions was optimized and addressed the choice of the manner of analytical signal generation, the influence of electrode modification with blocking agent, sensitivity dependence on the oligonucleotide sequence and the possibility of regeneration of sensing layer. Finally, the utility of proposed DNA sensors was tested by analysis in real samples.
Źródło:: Wiadomości Chemiczne; 2015, 69, 5-6; 325-336
0043-5104
2300-0295
Pojawia się w:: Wiadomości Chemiczne
Dostawca treści:: Biblioteka Nauki

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