Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

English (EN)·Polski (PL)·Yкраїнський (UK)
Przejdź do strony domowej biblioteki Centralna Biblioteka Wojskowa Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaCentralna Biblioteka Wojskowa

Wyszukujesz frazę "Luczyński, M." wg kryterium: Autor

  Lista filtrów
Wyrównaj
    Wyświetlanie 1-13 z 13
    Tytuł:
    Preliminary evaluation of mutagenic activity of two amino derivatives of cyclopenta[c]phenanthrene
    Autorzy:
    Łuczyński, M. K.
    Góra, M.
    Mielżyńska, D.
    Brzuzan, P.
    Tejs, S.
    Pirogowicz, P.
    Luczynski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363278.pdf
    Data publikacji:
    2007
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    test Amesa
    fenantren
    mutacje DNA
    WWA
    Ames test
    phenanthrene
    DNA mutations
    PAHs
    Opis:
    The mutagenicity of two, newly synthesized amino-derivatives of cyclopenta[c]phenanthrene (CP[c]Ph) was investigated in the Ames test using TA98 and TA100 histidine dependent Salmonella typhimurium strains. Neither of the examined compounds showed mutagenic activity without metabolic activation. The incorporation of an activation system caused high mutagenicity of CP[c]Ph derivatives in both S. typhimurium strains. The results are compared with the previous data on the mutagenic activity of unsubstituted CP[c]Ph and possible mechanisms of activation are discussed.
    Źródło:
    Environmental Biotechnology; 2007, 3, 1; 15-19
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genotoxicity of cyclopentha[c]phenanthrene and its two derivatives based on an in vitro micronucleus test
    Autorzy:
    Rudnicka, K.
    Łuczyński, M. K.
    Góra, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363298.pdf
    Data publikacji:
    2012
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    derivatives
    genotoxicity
    in vitro micronucleus test
    PAHs
    V79
    pochodne
    genotoksyczność
    test mikrojądrowy in vitro
    Opis:
    Environmental pollution causes a variety of health problems, including cancer. Many known pollutants have carcinogenic properties and polycyclic aromatic hydrocarbons (PAH) belong to this group. In this study, an in vitro culture of V79 cells of the Chinese hamster was subjected to three tested PAHs: 5-amino-2,3-dihydro-1H-cyclopenta-[c]phenanthrene (ACP[c]Ph), 5-amino-9-methoxy-2,3-dihydro-1H-cyclopenta[c]phenanthrene (AMCP[c]Ph) and cyclopenta[c]phenanthrene (CP[c]Ph). The in vitro micronucleus (MN) assay was applied in order to evaluate the genotoxic properties of the studied compounds. The highest genotoxic effect was observed for AMCP[c]Ph in a concentration of 0.02μg·ml-1. The genotoxic effect of the other two compounds was slightly lower.
    Źródło:
    Environmental Biotechnology; 2012, 8, 2; 63-66
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Relative quantification of CYP1A gene expression in whitefish (Coregonus lavaretus) exposed to benzo[a]pyrene
    Autorzy:
    Brzuzan, P.
    Jurczyk, Ł.
    Łuczyński, M. K.
    Góra, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363188.pdf
    Data publikacji:
    2005
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    ekspresja genu CYP1A
    sieja
    benzo(a)piren
    real-time PCR
    benzo(a)pyrene
    Coregonus lavaretus
    CYP1A gene expression
    RealTime PCR
    relative quantification
    Opis:
    The expression of CYP1A (cytochrome P4501A) can be induced by a number of aromatic compounds in teleost fishes. We developed a real-time PCR assay for measuring relative quantities (RQ) of CYP1A mRNA in whitefish (Coregonus lavaretus). To test for the usefulness of the assay we performed a treatment study, using benzo[a]pyrene (B[a]P) a model CYP1A inducer. Primers for the CYP1A gene were adapted from the literature, whereas those for [beta]-actin (endogenous control) were designed from a region that was found to be conserved among salmonid [beta]-actin genes. A group of hatchery raised whitefish, with an average body mass of 15 g and total length of 12 cm were given an intraperitoneal injection (10 mg/kg) of B[a]P in corn oil (2 mg B[a]P/ml corn oil) or corn oil alone (Control). After 48 h, whitefish liver, head kidney and brains were collected for mRNA isolation and analysis. In all three tissues sampled, CYP1A mRNA was affected by treatment with B[a]P. Head kidney tissue showed the greatest induction potential (RQ=11.00) from base levels (RQ=1.00), followed by liver (RQ=9.45), and brain (RQ=3.76). These results demonstrated that CYP1A was highly inducible by B[a]P in whitefish head kidney and liver, and to some extent, in brain tissue. The approach presented here has the advantage of providing rapid and accurate measures of CYP1A induction in various tissues of fish responding to PAH contaminant exposure.
    Źródło:
    Environmental Biotechnology; 2005, 1, 1; 11-15
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    CYP1A gene expression in adipose fin of rainbow trout (Oncorhynchus mykiss Walbaum) exposed to benzo[a]pyrene
    Autorzy:
    Brzuzan, P.
    Woźny, M.
    Łuczyński, M. K.
    Góra, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363276.pdf
    Data publikacji:
    2007
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    płetwa tłuszczowa
    benzo(a)piren
    CYP1A mRNA
    skrzela
    pstrąg tęczowy
    real-time PCR
    adipose fin
    benzo(a)pyrene
    gill
    rainbow trout
    Real-Time PCR
    Opis:
    Proximate to the environment, adipose fin of fish may be considered as a lipid storing tissue, and thus can be a target for either waterborne or dietary polycyclic aromatic compounds (PACs). We determined the effects of benzo[a]pyrene (B[a]P), a model PAC member, on CYP1A gene expression in adipose fin and compared that with the effects in gill of juvenile rainbow trout (Oncorhynchus mykiss Walbaum) using the quantitative reverse transcription polymerase chain reaction (Q-RT-PCR). The results of the study demonstrated that constitutive CYP1A mRNA was present in adipose fin of rainbow trout, but the transcripts were far less abundant than those in gill tissue. We confirmed high CYP1A gene induction potential of the gills in rainbow trout injected with benzo[a]pyrene, but also showed moderately and transiently induced CYP1A mRNA in adipose fin. The modest and transitory gene expression may preclude rainbow trout adipose fin CYP1A mRNA levels from using it as an indicator of sustained exposure of fish to the polycyclic aromatic compounds.
    Źródło:
    Environmental Biotechnology; 2007, 3, 1; 20-24
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Oxidative metabolism, mutagenic and carcinogenic properties of some polycyclic aromatic hydrocarbons
    Autorzy:
    Łuczyński, M. K.
    Góra, M.
    Brzuzan, P.
    Wilamowski, J.
    Kozik, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/363172.pdf
    Data publikacji:
    2005
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    metabolizm tlenowy
    właściwości mutagenne
    właściwości rakotwórcze
    wielopierścieniowe węglowodory aromatyczne
    oxidative metabolism
    mutagenic properties
    carcinogenic properties
    polycyclic aromatic hydrocarbons
    Opis:
    This attempt to synthesize the present state of knowledge on the correlation between the structure of chosen PAHs and their mutagenic or carcinogenic activity shows that the dependencies cannot be treated univocally. Extreme complexity of the subject, typical for investigations on the boundary between chemistry and biology, inclines one to search for new ways of synthesis of new compounds, which would be of interest for biologists studying the problem of chemical carcinogenesis. On the other hand, there is a strong need for elaborating a standardized test that would explicitly define the carcinogenic properties of a specific compound. The use of a variety of methods for the determination of carcinogenic potency of chemical compounds combined with simultaneous differentiation of structure of examined compounds, sometimes leads to contradictory conclusions. Correlation between mutagenic and carcinogenic activity of some compounds is also sometimes questionable. Modern molecular biology should allow for elaborating a method which would give a satisfactory and reliable answer to the fundamental question: is a specific compound carcinogenous for a human organism? And if so - to what extent?
    Źródło:
    Environmental Biotechnology; 2005, 1, 1; 16-28
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Benzo[a]pyrene and cyclopenta[c]phenanthrene suppress expression of p53 in head kidney of rainbow trout
    Autorzy:
    Brzuzan, P.
    Woźny, M.
    Góra, M.
    Łuczyński, M. K.
    Jabłońska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/363082.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    B[a]P
    CP[c]Ph
    ekspresja genów
    hnRNA
    pstrąg tęczowy
    poziom transkrypcji
    gene expression
    rainbow trout
    transcription rate
    Opis:
    Although p53, a protein of important tumor suppressive function, has been extensively studied in mammals, relatively little is known about the p53 pathways in lower vertebrates. Particularly, limited information exists on possible influences of environmental contaminants on the expression of the p53 gene in fish. In the current study, we assessed the effects of benzo[a]pyrene (B[a]P; potent tumor promoter) and cyclopenta[c]phenanthrene (CP[c]Ph; clastogenic agent) exposure on a 24h profile of p53 gene expression in head kidney of juvenile rainbow trout (Oncorhynchus mykiss). To analyze the p53 transcription rate, we developed protocol for the examination of both mRNA and heterogeneous nuclear (hn) RNA of the gene, using Real-Time RTPCR approach. The results show that both compounds are capable of suppressing p53 transcriptional activity within 12h of the treatment. Our finding supports the idea that structurally different PAHs may influence cell physiologic functions controlled by p53 in fish, in part, by down-regulating its RNA expression levels.
    Źródło:
    Environmental Biotechnology; 2011, 7, 1; 41-45
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Preliminary evaluation of ER- and AhR-mediated gene expression patterns in rainbow trout (Oncorhynchus mykiss) liver after short-term exposure to zearalenone in binary mixtures
    Autorzy:
    Woźny, M.
    Brzuzan, P.
    Wolińska, L.
    Góra, M.
    Łuczyński, M. K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363144.pdf
    Data publikacji:
    2010
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    receptor AhR
    benzo(a)piren
    ścieżka sygnałowa
    17beta-estradiol
    receptor estrogenowy
    zearalenone
    aryl hydrocarbon receptor
    benzo(a)pyrene
    crosstalk
    estrogen receptor
    Opis:
    While estrogenic properties of mycotoxin zearalenone (ZEA) has been an extensively studied issue, little is known about molecular background of its biological responses that cannot be simply explained by the estrogenic potential. The present study describes effects of ZEA (10mg*kg-1 body weight) in binary mixtures either with benzo[a]pyrene (B[a]P), or with 17β-estradiol (E2) on ER- and AhR-dependent gene expression in juvenile rainbow trout liver, evaluated using Real-Time qPCR. The study revealed dual nature of ZEA, as the treatment with this compound alone increased mRNA levels of both ER- and AhR-mediated gene expression. However, our results did not show any synergistic or additive effect of ZEA in binary mixures with E2 or B[a]P on studied gene expression levels. Whether the intriguing potential of ZEA to elicit distinct signals was a result of binding affinity to AhR or/and ER and AhR mutual receptor interactions, should be investigated in further experiments.
    Źródło:
    Environmental Biotechnology; 2010, 6, 1; 16-23
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cyclopenta[c]phenanthrene induction of CYP1A in brain of rainbow trout (Oncorhynchus mykiss)
    Autorzy:
    Brzuzan, P.
    Woźny, M.
    Łuczyński, M. K.
    Góra, M.
    Ciesielski, S.
    Kuźmiński, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/363272.pdf
    Data publikacji:
    2007
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    benzo(a)piren
    mózg
    CYP1A
    cyklopenta[c]fenantren
    absolutna kwantyfikacja mRNA
    pstrąg tęczowy
    benzo(a)pyrene
    brain
    cyclopenta[c]phenanthrene
    mRNA absolute quantification
    rainbow trout
    Opis:
    We assessed the effects of cyclopenta[c]phenanthrene (CP[c]Ph) and benzo[a]pyrene (B[a]P; positive control) on CYP1A gene expression in brain of juvenile rainbow trout (Oncorhynchus mykiss) using the quantitative reverse transcription polymerase chain reaction (Q-RT-PCR). A group of hatchery raised rainbow trout, with an average body mass of 49.4 g and total length of 15.5 cm were given an intraperitoneal injection (10 mg*kg-1) of either CP[c]Ph or B[a]P in corn oil (2 mg*mi-1 corn oil) or corn oil alone (control). After 24 and 48 h, trout brains were collected for mRNA isolation and analysis. After 24 hours of the exposure, only B[a]P-treated rainbow trout had 10-fold higher number of CYP1A transcripts (mean = 3.63*106 transcripts*µg-1 total RNA) than control fish (3.24*105 transcripts*µg-1 total RNA; Tukey test, P<0.05). After 48 hrs, significantly higher levels of CYP1A expression (Tukey test, P<0.001) were found in either CP[c]Ph- or B[a]P- induced group (1.45*106 and 6.92*106 transcriptsźµg-1 total RNA, respectively) over a control group (mean=1.41*105 transcripts*µg-1 total RNA). The finding that CYP1A in brain tissue was inducible by CP[c]Ph, a polycyclic aromatic hydrocarbon (PAH) of different than B[a]P planar characteristics, may further validate the use of rainbow trout brain CYP1A mRNA levels as a biomarker of PAH exposure.
    Źródło:
    Environmental Biotechnology; 2007, 3, 1; 10-14
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    2,4-diphenylthiophene induces mainly base pair mutation in Salmonella Typhimurium
    Autorzy:
    Budzikur, K. A.
    Mielżyńska-Švach, D.
    Góra, M.
    Chachaj, A.
    Pawłowski, M.
    Łuczyński, M. K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363086.pdf
    Data publikacji:
    2012
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    test Amesa
    związki heterocykliczne
    mutagenność
    siarkowe wielopierścieniowe węglowodory aromatyczne
    Ames test
    heterocyclic compounds
    mutagenicity
    sulfur-PAHs
    Opis:
    Heterocyclic aromatic compounds containing sulfur (S-HET), have been detected in air, soil, marine environment and freshwater sediment. Toxicity and mutagenicity data of this class of substances are scarce. The present study focuses on implications of two aryl thiophenes and their mutagenic properties in Salmonella/microsome test. In our experiment only 2,4-diphenylthiophene showed little mutagenic effect in both variants of activaction (+/-S9) in strain TA100. Thiophene ring joined to K-region of phenanthrene did not change the biological activity of 3,6-dimetoxyphenanthro [9,10-c]thiophene and this compound did not show mutagenic potency.
    Źródło:
    Environmental Biotechnology; 2012, 8, 1; 28-31
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mutagenicity induced in Salmonella strains TA98 and TA100 by diphenylthiophenes
    Autorzy:
    Budzikur, K. A.
    Góra, M.
    Chachaj, A.
    Mielżyńska-Švach, D.
    Tejs, S.
    Łuczyński, M. K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363118.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    test Amesa
    mutacja
    monomery tiofenowe
    Ames test
    mutation
    thiophene monomers
    Opis:
    Mutagenic properties of four different diphenylthiophenes: 3,4-diphenylthiophene, 3,4-di(4'-methylphenyl)thiophene, 3,4-di(4'-methoxyphenyl)thiophene and 3,4-di(4'-pentoxyphenyl)thiophene were investigated applying the Salmonella test. The research was done on two strains of Salmonella Typhimurium: TA98 and TA100, tested in two variants: with (+S9) and without (-S9) enzymatic activation. Only one compound 3,4-di(4'-methylphenyl)thiophene showed mutagenic activity when studied with metabolic activation (+S9) and its mutagenic rate (MR) score was 3.41 for the dose of 10.00µg*plate-1. Other studied compounds did not show any mutagenic activity (+/-S9) and their MR score did not exceed the threshold value of 2.0.
    Źródło:
    Environmental Biotechnology; 2011, 7, 2; 65-69
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Synthesis and fungistatic activity of aryl-substituted naphthalene- and indene-2-carbonitriles
    Autorzy:
    Kozik, B
    Burgieł, Z. J.
    Sepioł, J. J.
    Wilamowski, J.
    Łuczyński, M. K.
    Góra, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363192.pdf
    Data publikacji:
    2006
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    grzyby fitopatogenne
    WWA
    aktywność fungistatyczna
    ochrona roślin
    phytopathogenic fungi
    PAHs
    fungistatic activity
    plant protection
    Opis:
    4-Aryl-substituted 1-aminonaphthalene-2-carbonitriles and 3-phenyl-1-oxo-1H-indene-2-carbonitriles were prepared in a two--step synthesis from appropriate carbonyl compounds and were evaluated in vitro for growth-inhibiting activity against some phytopathogenic fungi: Fusarium culmorum, Penicillium expansum, Botrytis cinerea and Alternaria species. The results were compared with the activity of a commercial fungicide - captan. The highest fungistatic activity was revealed by 1-amino-4-phenylnaphthalene-2-carbonitrile. An efficient synthesis of 1-amino-4-arylnaphthalene-2-carbonitriles involves condensation of diarylacetaldehydes or 1,1-diarylacetones with malonodinitrile and cyclization of the obtained aryl-ylidenemalonodinitriles in concentrated sulfuric acid. The benzannulation reaction is accompanied by a quasi-aromatic rearrangement. The indenone derivatives were synthesized in the same manner from substituted benzophenones. As the formation of five-membered ring during the cyclization of diarylmethylidenemalonodinitriles was less effective, the rearrangement was not observed.
    Źródło:
    Environmental Biotechnology; 2006, 2, 1; 20-25
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Preliminary study on adverse effects of phenanthrene and its methyl and phenyl derivatives in larval zebrafish, Danio rerio
    Autorzy:
    Wolińska, L.
    Brzuzan, P.
    Woźny, M.
    Góra, M.
    Łuczyński, M. K.
    Podlasz, P.
    Kolwicz, S.
    Piasecka, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/363152.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    toksyczność ostra
    CYP1A
    cyp1b1
    geny vtg
    ekspresja mRNA
    stężenie nie wywołujące efektu
    WWA
    Real-Time qPCR
    acute toxicity
    cyp1a genes
    cyp1b1 genes
    vtg genes
    mRNA expression
    No Effect Concentration
    PAHs
    Opis:
    Toxic effects of polycyclic aromatic hydrocarbons (PAHs) have been extensively studied in fish, although knowledge concerning biological activities of phenanthrene and its derivatives remains still incomplete. The aim of this work was to evaluate lethal and sublethal effects of benzo(a)pyrene, phenanthrene and phenanthrene derivatives (1-methylphenanthrene, 4-methylphenanthrene, 1-phenylphenanthrene and 4-phenylphenanthrene) on zebrafish (Danio rerio) larvae. We conducted acute toxicity test, using 96h static renewal exposure to a series of the PAH concentrations (0.05, 0.50, 5.00, 50.00µmol*l-1), to determine the No Effect Concentration (NEC) value for each compound examined. The mean NEC estimates obtained in the study were 5.16۪.45µmol*l-1 (B[a]P), 4.88۪.13µmol*l-1 (Ph), 40.24䔰.93µmol*l-1 (1P-Ph), 47.92ۭ.61µmol*l-1 (1M-Ph), 24.31۱.33µmol*l-1 (4P-Ph) and 3.11۫.01µmol*l-1 (4M-Ph) and suggested the following order of PAH toxicities on Danio rerio larvae: 4M-Ph>Ph˜B[a]P>4PPhP-Ph>1M-Ph. To gain insight into possible molecular mechanisms of apparent toxicity of phenanthrene derivatives on zebrafish larvae, we examined mRNA expression of cyp1a, cyp1b1, and vtg genes in the larvae exposed for 48h to a PAH concentration of 0.50µmol*l-1. Whereas the larvae exposed to each tested PAH displayed many developmental abnormalities (i.e. pericardial and yolk sac edema, dorsal curvature, or tail malformations), no significant upregulation of cyp1a and cyp1b1 mRNA was observed, except for zebrafish exposed to B[a]P. However, significant reduction of vtg mRNA was observed in the larvae exposed to phenanthrene and its 4P- derivative. The results may contribute to the development of a new knowledge about effects of structurally diverse phenanthrene derivatives on vertebrate organisms.
    Źródło:
    Environmental Biotechnology; 2011, 7, 1; 26-33
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Assessment of mutagenic activity of methyl- and phenylphenanthrenes based on Salmonella test and micronucleus test
    Autorzy:
    Rudnicka, K.
    Tejs, S.
    Budzikur, K. A.
    Mielżyńska-Švach, D.
    Jakimiuk, E.
    Chachaj, A.
    Góra, M.
    Żelazna, K.
    Łuczyński, M. K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363178.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    Ames test
    in vitro
    micronucleus test
    mutagenicity
    phenanthrene
    test Amesa
    mutagenność
    test mikrojądrowy
    fenanatren
    Opis:
    Polycyclic aromatic hydrocarbons (PAHs) are widely spread environmental pollutants mainly originating from anthropogenic sources such as fossil fuel combustion, industries, and others. Although a large body of literature exists on the toxicity and carcinogenicity of PAHs, primarily benzo[a]pyrene, toxicity data for phenanthrene deriveratives are very limited. The main aim of the experiment was to investigate if there exists correlation between molecular structure and mutagenic activity of four phenanthrene derivatives: 1 methylphenanthrene, 4 methylphenanthrene, 1 phenylphenanthrene, and 4 phenylphenanthrene. An Ames assay using two strains of histidine dependent Salmonella Typhimurium (TA98 and TA100) was conducted to assess the mutagenic activity of studied compounds both in the presence (+S9) and in the absence (-S9) of an exogenous source of metabolic activation. The compounds were also tested in an in vitro chromosome aberration assay in which V-79 cells were exposed to the phenanthrene derivatives investigated both in the presence and in the absence of metabolic activation. The phenylphenanthrenes showed no mutagenic effect. These compounds occasionally induced significant decrease in the number of revertants in the Ames test. The greatest mutagenic effects were observed for 1 methylphenanthrene after metabolic activation (+S9). In the micronucleus test the greatest mutagenic effect was observed for 4 methylphenanthrene also in the presence of metabolic activation system. The results obtained are comparable to those reported earlier for the methylphenanthrenes.
    Źródło:
    Environmental Biotechnology; 2013, 9, 2; 65-71
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-13 z 13

      Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies