Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

English (EN)·Polski (PL)·Yкраїнський (UK)
Przejdź do strony domowej biblioteki Centralna Biblioteka Wojskowa Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaCentralna Biblioteka Wojskowa

Wyszukujesz frazę "cell culture" wg kryterium: Temat

  Lista filtrów
Wyrównaj
    Wyświetlanie 1-5 z 5
    Tytuł:
    Novel designs and technologies for cell engineering
    Autorzy:
    Ziółkowska, K.
    Chudy, M.
    Dybko, A.
    Brzózka, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/115526.pdf
    Data publikacji:
    2011
    Wydawca:
    Fundacja na Rzecz Młodych Naukowców
    Tematy:
    lab-on-a-chip
    multicellular tumor spheroid
    cancer
    in vitro cell culture
    cell engineering
    Opis:
    Microfluidic devices, such as lab-on-a-chip systems, are highly advantageous for cell engineering and cell based assays. It is a particularly useful approach for development of the in vitro cellular systems mimicking the in vivo environment. In this paper, a novel lab-on-a-chip device for three-dimensional human cell culture and anticancer drug testing is presented. Cells were cultured as Multicellular Tumor Spheroids (MCTS) — the best cancer tumor model developed so far. Diff erent designs were tested and novel technique of microfabrication in poly(dimethylsiloxane) was developed. MCTS were cultured in a system of polymeric microwells, with the network of microfluidic channels for culture medium flow. Design included optimal shear stress and proper nutrients supply for cultured cells. Final design provided MCTS culture for four weeks with the homeostasis-like state achievement, which is characteristic for the in vivo situation.
    Źródło:
    Challenges of Modern Technology; 2011, 2, 4; 54-60
    2082-2863
    2353-4419
    Pojawia się w:
    Challenges of Modern Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    ‘Lab-on-a-chip’ for cell engineering: towards cellular models mimicking in vivo
    Autorzy:
    Ziółkowska, K.
    Chudy, M.
    Dybko, A.
    Brzózka, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/115885.pdf
    Data publikacji:
    2011
    Wydawca:
    Fundacja na Rzecz Młodych Naukowców
    Tematy:
    Lab-on-a-chip
    microfluidic tools
    cancer
    cell culture
    Multicellular Tumor Spheroid (MCTS)
    Opis:
    One of the main scopes of modern cell engineering is development of cellular models that can replace animals in drug screening and toxicological tests, so called alternative methods. Construction of the alternative model is a very challenging task due to a richness of factors creating the in vivo environment. The monolayer cell culture — cultivation of adhesive cells on artificial surfaces such as glass or polymer — lack most of the in vivo-like interactions, but still is the only tool for the majority of applications. One of the most prospective approaches on mimicking in vivo environment is “Lab-on-a-chip” technology. Microfluidic devices offer lots of advantages over traditional in vitro culture, e.g. much higher cell volume-to-extracellular fluid volume ratio or possibility of regulation of hydrodynamic stress. This presentation aims to introduce latest advances of our team in microfluidic cell culture devices. Our novel approach is to cultivate three dimensional multicellular aggregates (spheroids) in microenvironments arranged in a microfluidic system. The geometry and materials of the system allow for cultivation, observation and analysis of multicellular spheroids. The results presented concern multicellular tumor spheroids (MCTS) rising from human cancer cells, which are considered to represent most of the conditionings of cancer tumor in vivo. The fully developed MCTS microdevice will be a reliable tool for anticancer drug screening, as the results most likely will be in a close accordance with the results obtained in vivo.
    Źródło:
    Challenges of Modern Technology; 2011, 2, 1; 79-82
    2082-2863
    2353-4419
    Pojawia się w:
    Challenges of Modern Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    An integrated gas removing system for microfluidic application. Design and evaluation
    Autorzy:
    Hofman, I. M.
    Ziółkowska, K.
    Dybko, A.
    Brzózka, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/115931.pdf
    Data publikacji:
    2013
    Wydawca:
    Fundacja na Rzecz Młodych Naukowców
    Tematy:
    gas removing system
    debubbler
    debubbling system
    lab-on-a-chip
    microfluidics
    long-term cell culture
    cell spheroids
    HT-29 cells
    Opis:
    Microfluidic systems are used in a wide range of applications, including medical diagnostics, cell engineering and bioanalytics. In this work we focused on “Lab-on-a-chip” microsystems for cell cultivation. A troublesome problem of gas bubbles entering microdevices causing signal interferences and cells damage was emphasized. A novel, integrated debubbler in the form of cylindrical traps covered with thin PDMS membrane was designed and manufactured. Demonstrated debubbler was successfully applied in a long-lasting culture of HT-29 cell aggregates.
    Źródło:
    Challenges of Modern Technology; 2013, 4, 3; 10-15
    2082-2863
    2353-4419
    Pojawia się w:
    Challenges of Modern Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Photodynamic therapy procedures in the microfluidic system
    Autorzy:
    Jędrych, E.
    Pawlicka, Z.
    Chudy, M.
    Dybko, A.
    Brzózka, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/115635.pdf
    Data publikacji:
    2010
    Wydawca:
    Fundacja na Rzecz Młodych Naukowców
    Tematy:
    photodynamic therapy (PDT)
    microfl uidic culture system
    PDMS
    adherent cell culture
    concentration gradient generator (CGG)
    5-aminolevulinic acid (ALA)
    Opis:
    Evaluation of the effi ciency of photodynamic therapy (PDT) in a hybrid microfl uidic culture system was studied. The geometry of the utilized microsystem for PDT procedures consists microchambers for cell culture and microchannels, which create a concentration gradient generator (CGG). 5-aminolevulinic acid (ALA) as a precursor of the photosensitizer was used. The geometry of the microchip allowed to test diff erent concentrations of ALA in a single assay. Evaluation of the effi ciency of photodynamic therapy was determined 24 hours after PDT procedure (irradiation with light which induced accumulated in carcinoma cells). The performed microsystem contained two independent micropatterns, that enables examination simultaneously various cell lines (carcinoma and normal) or various photosensitizers.
    Źródło:
    Challenges of Modern Technology; 2010, 1, 1; 30-33
    2082-2863
    2353-4419
    Pojawia się w:
    Challenges of Modern Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Microfluidic devices — application in anticancer studies
    Autorzy:
    Jędrych, E.
    Chudy, M.
    Dybko, A.
    Brzózka, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/115929.pdf
    Data publikacji:
    2012
    Wydawca:
    Fundacja na Rzecz Młodych Naukowców
    Tematy:
    microfluidic system
    PDMS
    adherent cell culture
    concentration gradient generator (CGG)
    cytotoxicity tests
    photodynamic therapy (PDT) procedures
    Opis:
    A rapidly growing pharmaceutical industry requires faster and more efficient ways to find and test new drugs. One of the new method for cell culture and examining the toxic effects of drugs is application of microfluidic systems. They provide new types of microenvironments and new methods for investigation of anticancer therapy. The use of microsystems is a solution that gives the opportunity to reduce not only cost and time, but also a number of tests on animals. In this paper we present designed and fabricated hybrid microfluidic systems which are applicable for cell culture, cell based cytotoxicity assays and photodynamic therapy procedures. Polydimethylsiloxane (PDMS) and sodium glass were used for fabrication of microdevices. The designed geometry of the microdevices includes cell culture microchambers and a concentration gradient generator (CGG). The CGG enables to obtain diff erent concentrations of tested drugs in a single step, which is a significant simplification of cytotoxicity assay procedure. In the designed microsystems three various cell lines (normal and carcinoma) were cultured and analyzed.
    Źródło:
    Challenges of Modern Technology; 2012, 3, 2; 3-5
    2082-2863
    2353-4419
    Pojawia się w:
    Challenges of Modern Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

      Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies