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Wyświetlanie 1-15 z 37
Tytuł:
Hantavirus RNA was not detected in Dermacentor reticulatus ticks
Autorzy:
Wojcik-Fatla, A.
Zajac, V.
Knap, J.P.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51730.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Opis:
A total of 190 Dermacentor reticulatus ticks (80 males, 110 females) collected on the territory of Ostrów Lubelski, Suchawa, Zalutyń and Kazimierz Dolny (Lublin Province, eastern Poland) were examined by reverse transcription PCR and nested PCR methods for the presence of hantavirus RNA. None of the examined Dermacentor reticulatus specimens showed the presence of the hantavirus-specific RNA in spite of using two pairs of primers and the clearly positive results obtained with the positive control. Thus, the hypothesis about the possible participation of ticks in the transmission of hantaviruses was not confirmed.
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 3
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A small scale survey of Leptospira in mammals from eastern Poland
Autorzy:
Wojcik-Fatla, A.
Zajac, V.
Sroka, J.
Piskorski, M.
Cisak, E.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51664.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 4
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Risk of Lyme disease at various sites and workplaces of forestry workers in eastern Poland
Autorzy:
Cisak, E.
Wojcik-Fatla, A.
Zajac, V.
Sroka, J.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/52094.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 3
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on Giardia duodenalis and Cryptosporidium spp. infection in veterinarians in Poland
Autorzy:
Wójcik-Fatla, A.
Sroka, J.
Zając, V.
Zwoliński, J.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/2081928.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
Giardia duodenalis
Cryptosporidium spp.
veterinarians
Polska
Opis:
The main aim of the study was to determine the risk of two selected zoonosis infections caused by Giardia duodenalis and Cryptosporidium spp. among an occupational group of veterinarians in Poland. Two hundred and ninety-seven samples of stool were tested for the presence of Giardia cysts and Cryptosporidium oocysts using Direct Fluorescent Assay (DFA). There were no positive results for Cryptosporidium. The presence of Giardia cysts was found in two samples of faeces (0.67%). The risk with regard to the parasites Giardia duodenalis and Cryptosporidium spp. seems to be low among the group of veterinarians.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 732-733
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of Toxoplasma gondii infection in cats in southwestern Poland
Autorzy:
Sroka, J.
Karamon, J.
Dutkiewicz, J.
Wójcik-Fatla, A.
Zając, V.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/2081746.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
Polska
PCR
seroprevalence
Toxoplasma gondii
IFAT
cats
oocysts
coproscopy
Opis:
Objective. An assessment of the prevalence of Toxoplasma gondii infection in cats from southwestern Poland using serology, coproscopy and PCR methods. Materials and method. In total, 208 cats (139 females and 68 males), aged 0.5–12 years (mean=2.6) from 25 localities in southwestern Poland were examined by indirect immunofluorescence assay (IFAT) to estimate the T. gondii serological status. Faecal samples of 41 cats were examined for the presence of oocysts/DNA T. gondii by microscopy and Real-time/nested PCR. After flotation (with NaNO3), pellets from faecal samples were disrupted by 10 cycles of freezing (liquid nitrogen) and warming. DNA was extracted using QIamp DNA Stool Mini Kit (Qiagen), according to the manufacturer’s instruction. Results. The positive results in IFAT for anti-T. gondii IgG and IgM antibodies were found in 143 of 208 tested cats (68.8%). Among positive results, 14.5%, 34.1% and 51.4% were detected in titre ranges 128–512, 1,000–2,000 and ≥ 4,000, respectively. In 23.1% of cat sera anti- T. gondii IgM antibodies were found. The prevalence of anti-Toxoplasma antibodies was significantly greater in older cats (>1 year) (83.5%) than in younger cats (48.3%) (P<0.05), in females (74.1%) than in males (58.8%) (P<0.05), and in cats kept outdoors than indoors (69.7% vs. 16.7%) (P<0.01). Among the 41 faecal samples examined, the presence of structures resembling T. gondii oocysts was found in 2 samples (4.9%), and for one of these samples (2.4% of the total) the result was also confirmed by PCR. Conclusions. T. gondii infection in domestic cats is highly prevalent in southwestern Poland. Information on the prevalence of infection in cats can be useful for assessing T. gondii environmental contamination and the risk for public health.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 3; 1-5
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hantavirus RNA not detected in Ixodes ricinus ticks
Autorzy:
Wojcik-Fatla, A.
Zajac, V.
Knap, J.P.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/49890.pdf
Data publikacji:
2011
Wydawca:
Instytut Medycyny Wsi
Tematy:
hantavirus
RNA
Ixodes ricinus
tick
epidemiology
polymerase chain reaction
Polska
Źródło:
Annals of Agricultural and Environmental Medicine; 2011, 18, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Occurrence of Leptospira DNA in water and soil samples collected in eastern Poland
Autorzy:
Wojcik-Fatla, A.
Zajac, V.
Wasinski, B.
Sroka, J.
Cisak, E.
Sawczyn, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51299.pdf
Data publikacji:
2014
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2014, 21, 4
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroprevalence of leptospirosis in rural populations inhabiting areas exposed andnot exposed to floods in eastern Poland
Autorzy:
Wasinski, B.
Sroka, J.
Wojcik-Fatla, A.
Zajac, V.
Cisak, E.
Knap, J.P.
Sawczyn, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/50328.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Opis:
Blood serum samples collected from randomly selected groups of 100 persons inhabiting rural community ‘A’ located in eastern Poland and exposed to floods by the Vistula river, and 98 persons inhabiting rural community ‘B’, also located in eastern Poland, but in the area not exposed to floods were examined by the microscopic agglutination test (MAT) for the presence of antibodies against 18 Leptospira serovars. Positive results showed 3% of serum samples collected from community ‘A’, while the seroprevalence in community ‘B’ was 9.2%, being insignificantly higher than in community ‘A’. For both examined communities (n=198), a significant positive correlation was found between the prevalence and titer of seropositive response and age of examined people (r=0.145, p=0.042). No significant differences were found between the prevalence of positive reactions in males and females (p>0.05). The reactions with 10 serovars of Leptospira (Australis, Autumnalis, Hebdomadis, Hardjo, Sejroe, Zanoni, Bataviae, Bratislava, Canicola and Grippotyphosa), belonging to 3 species (L. interrogans, L. borgpetersenii, L. kirschneri), were found in the examined communities. From both communities, of 12 persons demonstrating positive results in MAT, 9 showed reaction with one serovar, 2 with two serovars and 1 with three serovars. The highest titers found during the examination did not exceeded 800. In conclusion, our results suggest that there is only a slight, if any, hazard of an leptospirosis epidemic after the flood that afflicted eastern Poland in the year 2010 and the general epidemiological situation of leptospirosis in eastern Poland. Although there does not seem to be any cause for concern, further research is needed.
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Leptospirosis as a tick-borne disease? Detection of Leptospira spp. in Ixodes ricinus ticks in eastern Poland
Autorzy:
Wojcik-Fatla, A.
Zajac, V.
Cisak, E.
Sroka, J.
Sawczyn, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51864.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 4
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Repellents and acaricides as personal protection measures in the prevention of tick-borne diseases
Autorzy:
Cisak, E.
Wojcik-Fatla, A.
Zajac, V.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/50494.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 4
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimization of flotation, DNA extraction and PCR methods for detection of Toxoplasma gondii oocysts in cat faeces
Autorzy:
Sroka, J.
Karamon, J.
Dutkiewicz, J.
Wójcik-Fatla, A.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/2081971.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
flotation
Toxoplasma gondii
faeces
Real time PCR
nested PCR
cats
oocysts detection
Opis:
Introduction and objective. The aim of the study was to compare the effectiveness of selected oocysts concentration methods, DNA extraction protocols and PCR assays targeting the B1 gene, for the development of procedures which would be effective and useful in laboratory practice for the detection of T. gondii in faecal samples from cats. Materials and method. In order to compare the influence of the flotation fluids on microscopy and PCR detection of T. gondii, saturated solutions of saccharose, MgSO4, ZnSO4 and NaNO3 were used. To determine the sensitivity of PCR tests used: Real time PCR (RT) and nested PCR, water samples spiked with T. gondii tachyzoites and oocysts were tested. DNA was extracted using DNeasy Blood and Tissue Kit (Qiagen) (K1). The same PCR tests were used to assess the efficacy of T. gondii DNA detection in samples of cat faeces spiked with oocysts, using DNA extraction by a K1 set and a K2 set (QIamp DNA Stool Mini Kit, (Qiagen). Results. The initial results showed that NaNO3 was most useful as a flotation fluid due to the lack negative effect on the oocysts and amplification efficacy in PCR. The level of detection for water samples (100 μl) was determined as 100 tachyzoites and 1–50 oocysts in RT, and 2–20 oocysts in nested PCR. The limit of detection (LD) for stool samples (250 mg) spiked with oocysts, where the K1 set was used, determined as 250 and 5 oocysts in RT and nested PCR, respectively. For samples extracted with the K2 set, LD in RT was determined as 1–50 oocysts (depending on the variant) and 50 oocysts in nested PCR. Conclusions. The most effective methods for detection of T. gondii in cat faeces seem to be centrifugal flotation with NaNO3, followed by DNA extraction with removing of inhibitors (K2 set) and Real Time PCR targeting B1 gene.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 680-685
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Risk of tick-borne diseases in various categories of employment among forestry workers in eastern Poland
Autorzy:
Cisak, E.
Zajac, V.
Wojcik-Fatla, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51019.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 3
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Toxoplasma gondii infection in selected species of free-living animals in Poland
Autorzy:
Sroka, J.
Karamon, J.
Wójcik-Fatla, A.
Dutkiewicz, J.
Bilska-Zając, E.
Zając, V.
Piotrowska, W.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/2085130.pdf
Data publikacji:
2019
Wydawca:
Instytut Medycyny Wsi
Tematy:
Polska
Toxoplasma gondii
nested PCR
genotyping
free-living animals
Opis:
Introduction and objective. Free-living animals can play an important role as a reservoir of Toxoplasma gondi;, however, data concerning this issue in Poland are still limited.The aim of study was to assess the occurrence of T. gondii infection by using molecular methods in free-living animals in selected regions of Poland. Materials and method. Tissues samples of 396 animals (foxes, muskrats, birds, martens, badgers, polecats, raccoons, minks, raccoon dogs, otters, small rodents and insectivores, and grass snakes were collected from various regions of Poland. After samples digestion, DNA was isolated using QIAmp DNA Mini Kit (Qiagen). DNA extraction from small rodents and insectivores samples was performed without digestion. Next, nested PCR (B1 gene) and, for a part of nested PCR positive amplicons, RFLP PCR, were performed according to the method by Grigg and Boothroyd (2001). The other part of nested PCR positive DNA isolates were genotyped using 5 genetic markers: SAG1, SAG2 (5’- and 3’), SAG3, BTUB and GRA6, based on the method by Dubey et al. (2006). These PCR products were sequenced and compared with the NCBI database using Blast. Results. In total, in 50 of the 396 examined animals DNA of T. gondii was detected (12.6%). The highest percentages of positive results in PCR was obtained in martens (40.9%) and badgers (38.5%), lower in birds (27.3%) and the lowest in foxes (7.4%). The RFLP and multilocus PCR analysis showed the dominance of T. gondii clonal type II (or II/III). Conclusions. The results of this study indicate the frequent T. gondii infection among free-living animals in Poland, especially martens and badgers, which may indirectly indicate that these animals contribute to the spread of the parasite in the sylvatic environment in Poland. The genotyping analysis showed the dominance of T. gondii clonal type II (or II/III).
Źródło:
Annals of Agricultural and Environmental Medicine; 2019, 26, 4; 656-660
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on Lyme borreliosis focus in the Lublin region [eastern Poland]
Autorzy:
Cisak, E
Chmielewska-Badora, J.
Zwolinski, J.
Wojcik-Fatla, A.
Zajac, V.
Skorska, C.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51535.pdf
Data publikacji:
2008
Wydawca:
Instytut Medycyny Wsi
Tematy:
borreliosis
Polska
rural inhabitant
Lyme borreliosis
tick
seroepidemiology
Ixodes ricinus
Borrelia burgdorferi
clinical case
Lublin region
Źródło:
Annals of Agricultural and Environmental Medicine; 2008, 15, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 37

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