- Tytuł:
- Inkubacja doroslych osobnikow Ascaris suum w mediach chemicznie zdefiniowanych
- Autorzy:
-
Zoltkowska, K
Dmitryjuk, M.
Klonowska, A. - Powiązania:
- https://bibliotekanauki.pl/articles/840743.pdf
- Data publikacji:
- 1998
- Wydawca:
- Polskie Towarzystwo Parazytologiczne
- Tematy:
-
pozywki hodowlane
weglowodany
osobniki dorosle
sklad chemiczny
parazytologia
inkubacja
pasozyty
glista swinska
hodowla in vitro
zwiazki organiczne
Ascaris suum - Opis:
- Adult Ascaris suum were incubated at 37°C in Ringer solution for Ascaris (ARSb) and in ARSb with 0.2 % addition of glucose, maltose, trehalose, saccharose, starch and glycogen. Control worms were incubated in ARSb without natrium acetate (ARS0). The survival rate, motility and elasticity of each individual, changes of the body weight and glycogen contents in muscles were determined. The incubation in ARS0 was finished on the 7th day. The longest (11 days) lived the worms kept in the basic medium ARSb and ARSb with maltose. Regarding their influence on the survival rate of parasites, the rest sugars could be set in order as follows: glycogen and glucose (10 days), trehalose, saccharose and starch (9 days). In the media containing saccharose and starch worms showed reduced motility and elasticity. On the 7th day of incubation the glycogen level in muscles of control worms was reduced to 0.75% of its beginning concentration, whereas in muscles of Ascaris from media with glucose and trehalose over 50% and with maltose 43% of polysaccharide's reserves were preserved. A slight increase (10-15%) in average body weight of worms incubated with glucose, maltose and glycogen was observed, only in the control and medium with saccharose changes of worms' mass were significant, amounting to 30% and 80%, respectively.
- Źródło:
-
Annals of Parasitology; 1998, 44, 4; 671-679
0043-5163 - Pojawia się w:
- Annals of Parasitology
- Dostawca treści:
- Biblioteka Nauki