- Tytuł:
- Exogenous nitric oxide inhibits shedding of ADAM17 substrates
- Autorzy:
-
Bzowska, Monika
Stalińska, Krystyna
Mężyk-Kopeć, Renata
Wawro, Karolina
Duda, Katarzyna
Das, Sudipta
Bereta, Joanna - Powiązania:
- https://bibliotekanauki.pl/articles/1040593.pdf
- Data publikacji:
- 2009
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
shedding
ADAM17
TNF receptor 1
TNF
nitric oxide - Opis:
- Both ADAM17, the secretase responsible for the shedding of ectodomains of numerous membrane proteins including TNF and its receptors, as well as nitric oxide synthesized by inducible nitric oxide synthase play regulatory roles in inflammation and tumor progression. We analyzed the effect of endogenous and exogenous nitric oxide on the expression and activity of ADAM17 in murine endothelial cells and a monocyte/macrophage cell line. We found that endogenous nitric oxide influenced neither ADAM17 mRNA level nor the shedding of two ADAM17 substrates, TNF and TNFR1. Exogenous NO significantly diminished the release of TNF and TNFR1 without affecting the ADAM17 transcript level. Our data seem contrary to a previous report that showed the activation of ADAM17 by nitric oxide (Zhang et al., 2000, J Biol Chem 275: 15839-15844). We discuss potential mechanisms of NO-mediated inhibition of ectodomain shedding and possible reasons of discrepancy between our results and the previous report.
- Źródło:
-
Acta Biochimica Polonica; 2009, 56, 2; 325-335
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł