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Wyświetlanie 1-3 z 3
Tytuł:
Enzymatic activity of actinomycetes from the genus Streptomyces isolated from the bulk soil and rhizosphere of the Pinus sylvestris
Autorzy:
Golinska, P.
Dahm, H.
Powiązania:
https://bibliotekanauki.pl/articles/41609.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
enzyme activity
Actinomycetes
Streptomyces
isolation
bulk soil
forest soil
rhizosphere
Pinus sylvestris
chitinase
protease
pectinase
cellulase
Opis:
The enzymatic activity (chitinolytic, proteolytic, pectolytic andcellulolytic) of twenty strains of Streptomyces isolated from soil and rhizosphere of Scots pine was analyzed. Most strains produced chitinases, catalyzing the degradation of chitin, the main component of fungal cell walls including fungi pathogenic for plants. This activity was about 4 times higher in the presence of Fusariumoxysporum than Rhizoctonia solani mycelium or chitin flakes. The number of the proteolytic strains was also significant. In general, rhizosphere and soil organisms synthesizedcomparable amounts of these enzymes. Over half of the analyzed Streptomyces strains produced pectolytic enzymes (polygalacturonase, pectin lyase and pectate lyase). This property was more common among rhizosphere than among soil strains. The Streptomyces strains also showedcellulolytic activity (endocellulases, exocellulases) – enzymes decomposing basic components of cell walls of plant and lower fungi (cellulose). The cellulolytic activity was differentiated and depended on the Streptomyces strain. Conclusion of our studies is that Streptomyces are the microorganisms more chitinolytic andproteolytic than pectolytic and cellulolytic.
Źródło:
Dendrobiology; 2011, 65
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Occurrence of actinomycetes in forest soil
Autorzy:
Golinska, P.
Dahm, H.
Powiązania:
https://bibliotekanauki.pl/articles/41633.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Opis:
Actinomycetes from the genus Streptomyces are Gram-positive bacteria commonly and in large numbers isolated from the soil, which is their natural habitat. Due to their metabolic activity and high adaptative capabilities they are an important link in the circulation of matter and energy. They play an important role in the formation of bioactive metabolites, mainly antibiotic compounds. In contrast to numerous elaborations concerning actinomycetes of cultivated soils, little is known about their occurrence in forest soils. The main factor limiting actinomycete development in forest soils is believed to be the low pH, as the development of most actinomycetes is facilitated by a neutral or alkaline soil reaction. However, these microorganisms have also been isolated from strongly acidic soils, which was confirmed in our studies. The pH value of analysed soils was in the range 4.0–4.3 (4.0 for bulk soil of alder; 4.1 for bulk soil of Scots pine; 4.3 for bulk soil of birch). Root soil of the analyzed trees contained more microorganisms than soil outside the range of the roots. This also concerned actinomycetes, eubacteria as well as saprophytic fungi. In our investigations the greatest number of eubacteria were connected with the alder (bulk soil, rhizosphere and rhizoplane), and the smallest number with the pine, whereas actinomycetes were the most numerous in the birch rhizosphere. The analyzed actinomycete strains were identified to 18 taxa, with a dominant species Streptomyces exfoliatus.
Źródło:
Dendrobiology; 2011, 66
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antagonistic properties of Streptomyces isolated from forest soils against fungal pathogens of pine seedlings
Autorzy:
Golinska, P.
Dahm, H.
Powiązania:
https://bibliotekanauki.pl/articles/41547.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Opis:
Biological control of plant diseases has received worldwide attention in recent years mainly as a response to use of hazardous chemicals in the environment. Soil actinomycetes particularly Streptomyces spp. enhance soil fertility and have antagonistic activity against wide range of soil-borne plant pathogens. In study of antifungal activity of streptomycetes against plant fungal-pathogens Fusarium oxysporum (Schlecht.), F. culmorum (Wm. G. Sm.) Sacc. and Rhizoctonia solani (Kühn) causing damping-off of pine seedlings, in vitro and in vivo tests were carried out. Two types of in vitro tests in mixed cultures of actinomycetes and fungi were performed. In majority of the cases, an inhibitory effect of actinomycetes on fungi was noticed. Three isolates (SG9, SR2, SR4) were highly active against pathogenic fungi and were chosen for exopolysaccharides extraction and in vivo tests. The sensitivity to exopolysaccharide action was noted in fungi from the genus Fusarium. Fusarium oxysporum showed a higher sensitivity to the analyzed exopolysaccharides than F. culmorum. Biocontrol interactions both in sterile and non-sterile soil were found for the actinomycete Streptomyces cyaneus (strain SG9) isolated from root free soil of the pine. The latest experiment confirmed the strong pathogenicity of the fungus Rhizoctonia solani. Antifungal properties of Streptomyces cyaneus (strain SG9) need further comprehensive studies.
Źródło:
Dendrobiology; 2013, 69
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-3 z 3

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