- Tytuł:
- Analysis of microsatellite instability and loss of heterozygosity in breast cancer with the use of a well characterized multiplex system.
- Autorzy:
-
Powierska-Czarny, Jolanta
Miścicka-Śliwka, Danuta
Czarny, Jakub
Grzybowski, Tomasz
Woźniak, Marcin
Drewa, Gerard
Czechowicz, Włodzimierz
Sir, Jan - Powiązania:
- https://bibliotekanauki.pl/articles/1043412.pdf
- Data publikacji:
- 2003
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
microsatellite instability
short tandem repeats
multiplex DNA typing
breast cancer - Opis:
- Analysis of microsatellite instability (MI) and loss of heterozygosity (LOH) is recommended for screening patients with sporadic and hereditary malignancies. This study shows an application of a fluorescent hexaplex PCR system for microsatellite typing on A.L.F. DNA Sequencer (Pharmacia Biotech). This technique detects changes in microsatellites providing a time-efficient, reliable and accurate method for MI and LOH analyses. The Fragment Manager software was used for automated size calculation and quantitation of DNA fragments, enabling rapid and precise measurement of allelic ratios. We examined 70 breast cancer and 70 control DNA specimens, classified all the patterns of microsatellite alterations, and set up MI and LOH assessment criteria for the automated multiplex fluorescent method.
- Źródło:
-
Acta Biochimica Polonica; 2003, 50, 4; 1195-1203
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł