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Wyświetlanie 1-15 z 28
Tytuł:
Assessment of viability of the nematode eggs (Ascaris, Toxocara, Trichuris) in sewage sludge with the use of LIVE/DEAD Bacterial Viability Kit
Autorzy:
Dabrowska, J.
Zdybel, J.
Karamon, J.
Kochanowski, M.
Stojecki, K.
Cencek, T.
Klapec, T.
Powiązania:
https://bibliotekanauki.pl/articles/50994.pdf
Data publikacji:
2014
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2014, 21, 1
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular diagnostics of Sarcocystis spp. infections
Autorzy:
Stojecki, K.
Karamon, J.
Sroka, J.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/2088002.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
molecular diagnostics
Sarcocystis
infection
sarcocystosis
polymerase chain reaction
Opis:
Protozoa of the genus Sarcocystis (phylum Apicomplexa, family Sarcocystidae) is one of the most common parasites affecting animals. Interspecies diagnostic of Sarcocystis genus was based on electron microscopy for many years. Because of absence of visible differences between species with reachable magnifications, light microscopy is useless. In many cases serological diagnostic method have lack of sensitivity. A variety of molecular methods have been developed and used to detect and identify Sarcocystis spp. and to assess the genetic diversity among this protozoan from different population/hosts. Nowadays, molecular diagnostic is the common, time/cost effective method used all over the world to interspecies differentiation.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of Toxoplasma gondii infection in cats in southwestern Poland
Autorzy:
Sroka, J.
Karamon, J.
Dutkiewicz, J.
Wójcik-Fatla, A.
Zając, V.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/2081746.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
Polska
PCR
seroprevalence
Toxoplasma gondii
IFAT
cats
oocysts
coproscopy
Opis:
Objective. An assessment of the prevalence of Toxoplasma gondii infection in cats from southwestern Poland using serology, coproscopy and PCR methods. Materials and method. In total, 208 cats (139 females and 68 males), aged 0.5–12 years (mean=2.6) from 25 localities in southwestern Poland were examined by indirect immunofluorescence assay (IFAT) to estimate the T. gondii serological status. Faecal samples of 41 cats were examined for the presence of oocysts/DNA T. gondii by microscopy and Real-time/nested PCR. After flotation (with NaNO3), pellets from faecal samples were disrupted by 10 cycles of freezing (liquid nitrogen) and warming. DNA was extracted using QIamp DNA Stool Mini Kit (Qiagen), according to the manufacturer’s instruction. Results. The positive results in IFAT for anti-T. gondii IgG and IgM antibodies were found in 143 of 208 tested cats (68.8%). Among positive results, 14.5%, 34.1% and 51.4% were detected in titre ranges 128–512, 1,000–2,000 and ≥ 4,000, respectively. In 23.1% of cat sera anti- T. gondii IgM antibodies were found. The prevalence of anti-Toxoplasma antibodies was significantly greater in older cats (>1 year) (83.5%) than in younger cats (48.3%) (P<0.05), in females (74.1%) than in males (58.8%) (P<0.05), and in cats kept outdoors than indoors (69.7% vs. 16.7%) (P<0.01). Among the 41 faecal samples examined, the presence of structures resembling T. gondii oocysts was found in 2 samples (4.9%), and for one of these samples (2.4% of the total) the result was also confirmed by PCR. Conclusions. T. gondii infection in domestic cats is highly prevalent in southwestern Poland. Information on the prevalence of infection in cats can be useful for assessing T. gondii environmental contamination and the risk for public health.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 3; 1-5
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of the effectiveness of various parasitological methods in detecting nematode eggs in different types of soil
Autorzy:
Zdybel, J.M.
Sroka, J.
Karamon, J.
Bilska-Zając, E.
Wójcik-Fatla, A.
Kłapeć, T.
Skowron, P.
Siebielec, G.
Jadczyszyn, T.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/28763034.pdf
Data publikacji:
2023
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2023, 30, 3; 425-431
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Piroplazmoza koni
Equine piroplasmosis
Autorzy:
Karamon, J.
Sroka, J.
Cencek, T.
Zdybel, J.
Powiązania:
https://bibliotekanauki.pl/articles/862265.pdf
Data publikacji:
2010
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
konie
choroby zwierzat
choroby pasozytnicze
pierwotniaki
Babesia caballi
cykl rozwojowy
patogeneza
objawy chorobowe
diagnostyka
zwalczanie chorob zwierzat
leczenie
Źródło:
Życie Weterynaryjne; 2010, 85, 12
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Occurrence of Trichinella spp. in rats on pig farms
Autorzy:
Bliska-Zając, E.
Różycki, M.
Antolak, E.
Bełcik, A.
Grądziel-Krukowska, K.
Karamon, J.
Sroka, J.
Zdybel, J.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/2081958.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
Trichinella spp.
rats
pig farm
Opis:
Introduction. The highest risk of trichinellosis for human is considered in eating meat products containing live larvae, mostly from wild boars or pigs. Spreading of Trichinella spp. may occur in various ways, one of which is transmission by vectors. The rat is considered to be the most common vector for Trichinella parasite. The population of rats living on pig farms can play an important role in maintaining or spreading the parasite to other animals. Objective. The aim of presented survey was to investigate the occurrence of Trichinella spp. in rats on farms with pigs infected with this parasite. Materials and method. From pig farms selected for study, the muscles of collected rats were investigated by magnetic stirrer digestion method to assess occurrence of Trichinella in the rat population. Isolated Trichinella parasites were identified under stereomicroscope and multiplex PCR were performed for species identification. Results. Rats infected with Trichinella spp. were discovered on three of five investigated pig farms. The mean extent of invasion in rats from the studied farms was 23.33%. The calculated medium intensity of invasion was 4.09 lpg (larvae per gram) (SD 5.41). All larvae of Trichinella discovered from rats were identified as T.spiralis. Conclusions. The results obtained indicate that in farms with a high prevalence of Trichinella invasion in pigs there are very likely to be found rats infected by this nematode. This suggests possibility to maintain the invasion in herd and spread into neighborhood farms.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 698-700
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Epidemiological survey in Łęczyńsko-Włodawskie Lake District of eastern Poland reveals new evidence of zoonotic potential of Giardia intestinalis
Autorzy:
Stojecki, K.
Sroka, J.
Cencek, T.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/50854.pdf
Data publikacji:
2015
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2015, 22, 4
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of selected stool concentration techniques on the effectiveness of PCR examination in Giardia intestinalis diagnostics
Autorzy:
Stojecki, K.
Sroka, J.
Karamon, J.
Kusyk, P.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/32229.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Giardia intestinalis is a widespread parasitic protozoa which has great significance as a public health threat. Molecular diagnostics of stool sample can be unreliable because of the presence of inhibitors of enzymatic reactions. The aim of this study was to determine the effectiveness of selected pre-treatment methods of fecal samples for further PCR-based diagnostics of G. intestinalis, and the effect of each component of pre-treatment solutions on PCR reactions. Seven stool concentration techniques were compared. The results showed that the most efficient concentration method for stool sample preparation for detection of G. intestinalis by PCR is centrifugal flotation with Percoll (with saturated NaNO₃ as the flotation solution). This method is relatively inexpensive, less labor-intensive, and suitable for epidemiological monitoring and clinical investigations.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Preliminary assessment of usefulness of cELISA test for screening pig and cattle populations for presence of antibodies against Toxoplasma gondii
Autorzy:
Sroka, J.
Karamon, J.
Cencek, T.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/50742.pdf
Data publikacji:
2011
Wydawca:
Instytut Medycyny Wsi
Tematy:
usefulness
cELISA test
screening
pig
cattle
animal population
serology
antibody
Toxoplasma gondii
toxoplasmosis
Źródło:
Annals of Agricultural and Environmental Medicine; 2011, 18, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimization of flotation, DNA extraction and PCR methods for detection of Toxoplasma gondii oocysts in cat faeces
Autorzy:
Sroka, J.
Karamon, J.
Dutkiewicz, J.
Wójcik-Fatla, A.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/2081971.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
flotation
Toxoplasma gondii
faeces
Real time PCR
nested PCR
cats
oocysts detection
Opis:
Introduction and objective. The aim of the study was to compare the effectiveness of selected oocysts concentration methods, DNA extraction protocols and PCR assays targeting the B1 gene, for the development of procedures which would be effective and useful in laboratory practice for the detection of T. gondii in faecal samples from cats. Materials and method. In order to compare the influence of the flotation fluids on microscopy and PCR detection of T. gondii, saturated solutions of saccharose, MgSO4, ZnSO4 and NaNO3 were used. To determine the sensitivity of PCR tests used: Real time PCR (RT) and nested PCR, water samples spiked with T. gondii tachyzoites and oocysts were tested. DNA was extracted using DNeasy Blood and Tissue Kit (Qiagen) (K1). The same PCR tests were used to assess the efficacy of T. gondii DNA detection in samples of cat faeces spiked with oocysts, using DNA extraction by a K1 set and a K2 set (QIamp DNA Stool Mini Kit, (Qiagen). Results. The initial results showed that NaNO3 was most useful as a flotation fluid due to the lack negative effect on the oocysts and amplification efficacy in PCR. The level of detection for water samples (100 μl) was determined as 100 tachyzoites and 1–50 oocysts in RT, and 2–20 oocysts in nested PCR. The limit of detection (LD) for stool samples (250 mg) spiked with oocysts, where the K1 set was used, determined as 250 and 5 oocysts in RT and nested PCR, respectively. For samples extracted with the K2 set, LD in RT was determined as 1–50 oocysts (depending on the variant) and 50 oocysts in nested PCR. Conclusions. The most effective methods for detection of T. gondii in cat faeces seem to be centrifugal flotation with NaNO3, followed by DNA extraction with removing of inhibitors (K2 set) and Real Time PCR targeting B1 gene.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 680-685
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wystepowanie tasiemcow Echinococcus multilocularis u lisow w zachodniej czesci Polski
Autorzy:
Karamon, J.
Sroka, J.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/837124.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
pasozyty zwierzat
tasiemce
Echinococcus multilocularis
wystepowanie
inwazja pasozytnicza
lisy
Polska Zachodnia
woj.zachodniopomorskie
woj.lubuskie
woj.dolnoslaskie
Źródło:
Annals of Parasitology; 2010, 56, 3; 222
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Toxoplasma gondii infection in selected species of free-living animals in Poland
Autorzy:
Sroka, J.
Karamon, J.
Wójcik-Fatla, A.
Dutkiewicz, J.
Bilska-Zając, E.
Zając, V.
Piotrowska, W.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/2085130.pdf
Data publikacji:
2019
Wydawca:
Instytut Medycyny Wsi
Tematy:
Polska
Toxoplasma gondii
nested PCR
genotyping
free-living animals
Opis:
Introduction and objective. Free-living animals can play an important role as a reservoir of Toxoplasma gondi;, however, data concerning this issue in Poland are still limited.The aim of study was to assess the occurrence of T. gondii infection by using molecular methods in free-living animals in selected regions of Poland. Materials and method. Tissues samples of 396 animals (foxes, muskrats, birds, martens, badgers, polecats, raccoons, minks, raccoon dogs, otters, small rodents and insectivores, and grass snakes were collected from various regions of Poland. After samples digestion, DNA was isolated using QIAmp DNA Mini Kit (Qiagen). DNA extraction from small rodents and insectivores samples was performed without digestion. Next, nested PCR (B1 gene) and, for a part of nested PCR positive amplicons, RFLP PCR, were performed according to the method by Grigg and Boothroyd (2001). The other part of nested PCR positive DNA isolates were genotyped using 5 genetic markers: SAG1, SAG2 (5’- and 3’), SAG3, BTUB and GRA6, based on the method by Dubey et al. (2006). These PCR products were sequenced and compared with the NCBI database using Blast. Results. In total, in 50 of the 396 examined animals DNA of T. gondii was detected (12.6%). The highest percentages of positive results in PCR was obtained in martens (40.9%) and badgers (38.5%), lower in birds (27.3%) and the lowest in foxes (7.4%). The RFLP and multilocus PCR analysis showed the dominance of T. gondii clonal type II (or II/III). Conclusions. The results of this study indicate the frequent T. gondii infection among free-living animals in Poland, especially martens and badgers, which may indirectly indicate that these animals contribute to the spread of the parasite in the sylvatic environment in Poland. The genotyping analysis showed the dominance of T. gondii clonal type II (or II/III).
Źródło:
Annals of Agricultural and Environmental Medicine; 2019, 26, 4; 656-660
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rola dochodzeń epidemiologicznych w aktualnej sytuacji epidemiologicznej włośnicy w Polsce
The role of epidemiological investigations in the current epidemiology of trichinellosis in Poland
Autorzy:
Bilska-Zajac, E.
Rozycki, M.
Karamon, J.
Sroka, J.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/858910.pdf
Data publikacji:
2019
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Źródło:
Życie Weterynaryjne; 2019, 94, 06
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The changing epidemiological situation of Echinococcus multilocularis in red foxes in Poland
Autorzy:
Karamon, J.
Samorek-Pierog, M.
Kochanowski, M.
Dabrowska, J.
Rozycki, M.
Bilska-Zajac, E.
Chmurzynska, E.
Sroka, J.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/5787.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
epidemiological situation
changing
Echinococcus multilocularis
prevalence
animal infection
fox
red fox
Polska
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Echinococcus multilocularis w Polsce - sytuacja epizootyczna u lisów wskaźnikiem ryzyka zarażenia ludzi
Echinococcus multilocularis in Poland - epizootic situation in red foxes as the indicator of infection risk for humans
Autorzy:
Karamon, J.
Kochanowski, M.
Dabrowska, J.
Rozycki, M.
Bilska-Zajac, E.
Sroka, J.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/858418.pdf
Data publikacji:
2015
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Źródło:
Życie Weterynaryjne; 2015, 90, 09
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 28

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