Temat: real time PCR - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: CYP1A gene expression in adipose fin of rainbow trout (Oncorhynchus mykiss Walbaum) exposed to benzo[a]pyrene
Autorzy:: Brzuzan, P.
Woźny, M.
Łuczyński, M. K.
Góra, M.
Powiązania:: https://bibliotekanauki.pl/articles/363276.pdf
Data publikacji:: 2007
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:: płetwa tłuszczowa
benzo(a)piren
CYP1A mRNA
skrzela
pstrąg tęczowy
real-time PCR
adipose fin
benzo(a)pyrene
gill
rainbow trout
Real-Time PCR
Opis:: Proximate to the environment, adipose fin of fish may be considered as a lipid storing tissue, and thus can be a target for either waterborne or dietary polycyclic aromatic compounds (PACs). We determined the effects of benzo[a]pyrene (B[a]P), a model PAC member, on CYP1A gene expression in adipose fin and compared that with the effects in gill of juvenile rainbow trout (Oncorhynchus mykiss Walbaum) using the quantitative reverse transcription polymerase chain reaction (Q-RT-PCR). The results of the study demonstrated that constitutive CYP1A mRNA was present in adipose fin of rainbow trout, but the transcripts were far less abundant than those in gill tissue. We confirmed high CYP1A gene induction potential of the gills in rainbow trout injected with benzo[a]pyrene, but also showed moderately and transiently induced CYP1A mRNA in adipose fin. The modest and transitory gene expression may preclude rainbow trout adipose fin CYP1A mRNA levels from using it as an indicator of sustained exposure of fish to the polycyclic aromatic compounds.
Źródło:: Environmental Biotechnology; 2007, 3, 1; 20-24
1734-4964
Pojawia się w:: Environmental Biotechnology
Dostawca treści:: Biblioteka Nauki

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Tytuł:: Relative quantification of CYP1A gene expression in whitefish (Coregonus lavaretus) exposed to benzo[a]pyrene
Autorzy:: Brzuzan, P.
Jurczyk, Ł.
Łuczyński, M. K.
Góra, M.
Powiązania:: https://bibliotekanauki.pl/articles/363188.pdf
Data publikacji:: 2005
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:: ekspresja genu CYP1A
sieja
benzo(a)piren
real-time PCR
benzo(a)pyrene
Coregonus lavaretus
CYP1A gene expression
RealTime PCR
relative quantification
Opis:: The expression of CYP1A (cytochrome P4501A) can be induced by a number of aromatic compounds in teleost fishes. We developed a real-time PCR assay for measuring relative quantities (RQ) of CYP1A mRNA in whitefish (Coregonus lavaretus). To test for the usefulness of the assay we performed a treatment study, using benzo[a]pyrene (B[a]P) a model CYP1A inducer. Primers for the CYP1A gene were adapted from the literature, whereas those for [beta]-actin (endogenous control) were designed from a region that was found to be conserved among salmonid [beta]-actin genes. A group of hatchery raised whitefish, with an average body mass of 15 g and total length of 12 cm were given an intraperitoneal injection (10 mg/kg) of B[a]P in corn oil (2 mg B[a]P/ml corn oil) or corn oil alone (Control). After 48 h, whitefish liver, head kidney and brains were collected for mRNA isolation and analysis. In all three tissues sampled, CYP1A mRNA was affected by treatment with B[a]P. Head kidney tissue showed the greatest induction potential (RQ=11.00) from base levels (RQ=1.00), followed by liver (RQ=9.45), and brain (RQ=3.76). These results demonstrated that CYP1A was highly inducible by B[a]P in whitefish head kidney and liver, and to some extent, in brain tissue. The approach presented here has the advantage of providing rapid and accurate measures of CYP1A induction in various tissues of fish responding to PAH contaminant exposure.
Źródło:: Environmental Biotechnology; 2005, 1, 1; 11-15
1734-4964
Pojawia się w:: Environmental Biotechnology
Dostawca treści:: Biblioteka Nauki

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