Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Wyszukujesz frazę "pstrag teczowy" wg kryterium: Temat


Wyświetlanie 1-3 z 3
Tytuł:
Benzo[a]pyrene and cyclopenta[c]phenanthrene suppress expression of p53 in head kidney of rainbow trout
Autorzy:
Brzuzan, P.
Woźny, M.
Góra, M.
Łuczyński, M. K.
Jabłońska, A.
Powiązania:
https://bibliotekanauki.pl/articles/363082.pdf
Data publikacji:
2011
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
B[a]P
CP[c]Ph
ekspresja genów
hnRNA
pstrąg tęczowy
poziom transkrypcji
gene expression
rainbow trout
transcription rate
Opis:
Although p53, a protein of important tumor suppressive function, has been extensively studied in mammals, relatively little is known about the p53 pathways in lower vertebrates. Particularly, limited information exists on possible influences of environmental contaminants on the expression of the p53 gene in fish. In the current study, we assessed the effects of benzo[a]pyrene (B[a]P; potent tumor promoter) and cyclopenta[c]phenanthrene (CP[c]Ph; clastogenic agent) exposure on a 24h profile of p53 gene expression in head kidney of juvenile rainbow trout (Oncorhynchus mykiss). To analyze the p53 transcription rate, we developed protocol for the examination of both mRNA and heterogeneous nuclear (hn) RNA of the gene, using Real-Time RTPCR approach. The results show that both compounds are capable of suppressing p53 transcriptional activity within 12h of the treatment. Our finding supports the idea that structurally different PAHs may influence cell physiologic functions controlled by p53 in fish, in part, by down-regulating its RNA expression levels.
Źródło:
Environmental Biotechnology; 2011, 7, 1; 41-45
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
CYP1A gene expression in adipose fin of rainbow trout (Oncorhynchus mykiss Walbaum) exposed to benzo[a]pyrene
Autorzy:
Brzuzan, P.
Woźny, M.
Łuczyński, M. K.
Góra, M.
Powiązania:
https://bibliotekanauki.pl/articles/363276.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
płetwa tłuszczowa
benzo(a)piren
CYP1A mRNA
skrzela
pstrąg tęczowy
real-time PCR
adipose fin
benzo(a)pyrene
gill
rainbow trout
Real-Time PCR
Opis:
Proximate to the environment, adipose fin of fish may be considered as a lipid storing tissue, and thus can be a target for either waterborne or dietary polycyclic aromatic compounds (PACs). We determined the effects of benzo[a]pyrene (B[a]P), a model PAC member, on CYP1A gene expression in adipose fin and compared that with the effects in gill of juvenile rainbow trout (Oncorhynchus mykiss Walbaum) using the quantitative reverse transcription polymerase chain reaction (Q-RT-PCR). The results of the study demonstrated that constitutive CYP1A mRNA was present in adipose fin of rainbow trout, but the transcripts were far less abundant than those in gill tissue. We confirmed high CYP1A gene induction potential of the gills in rainbow trout injected with benzo[a]pyrene, but also showed moderately and transiently induced CYP1A mRNA in adipose fin. The modest and transitory gene expression may preclude rainbow trout adipose fin CYP1A mRNA levels from using it as an indicator of sustained exposure of fish to the polycyclic aromatic compounds.
Źródło:
Environmental Biotechnology; 2007, 3, 1; 20-24
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cyclopenta[c]phenanthrene induction of CYP1A in brain of rainbow trout (Oncorhynchus mykiss)
Autorzy:
Brzuzan, P.
Woźny, M.
Łuczyński, M. K.
Góra, M.
Ciesielski, S.
Kuźmiński, H.
Powiązania:
https://bibliotekanauki.pl/articles/363272.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
benzo(a)piren
mózg
CYP1A
cyklopenta[c]fenantren
absolutna kwantyfikacja mRNA
pstrąg tęczowy
benzo(a)pyrene
brain
cyclopenta[c]phenanthrene
mRNA absolute quantification
rainbow trout
Opis:
We assessed the effects of cyclopenta[c]phenanthrene (CP[c]Ph) and benzo[a]pyrene (B[a]P; positive control) on CYP1A gene expression in brain of juvenile rainbow trout (Oncorhynchus mykiss) using the quantitative reverse transcription polymerase chain reaction (Q-RT-PCR). A group of hatchery raised rainbow trout, with an average body mass of 49.4 g and total length of 15.5 cm were given an intraperitoneal injection (10 mg*kg-1) of either CP[c]Ph or B[a]P in corn oil (2 mg*mi-1 corn oil) or corn oil alone (control). After 24 and 48 h, trout brains were collected for mRNA isolation and analysis. After 24 hours of the exposure, only B[a]P-treated rainbow trout had 10-fold higher number of CYP1A transcripts (mean = 3.63*106 transcripts*µg-1 total RNA) than control fish (3.24*105 transcripts*µg-1 total RNA; Tukey test, P<0.05). After 48 hrs, significantly higher levels of CYP1A expression (Tukey test, P<0.001) were found in either CP[c]Ph- or B[a]P- induced group (1.45*106 and 6.92*106 transcriptsźµg-1 total RNA, respectively) over a control group (mean=1.41*105 transcripts*µg-1 total RNA). The finding that CYP1A in brain tissue was inducible by CP[c]Ph, a polycyclic aromatic hydrocarbon (PAH) of different than B[a]P planar characteristics, may further validate the use of rainbow trout brain CYP1A mRNA levels as a biomarker of PAH exposure.
Źródło:
Environmental Biotechnology; 2007, 3, 1; 10-14
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-3 z 3

    Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies