- Tytuł:
- Quantification of triazine herbicides using chloroplasts in conjunction with thin-layer chromatography
- Autorzy:
-
Broszat, M.
Ernst, H.
Spangenberg, B. - Powiązania:
- https://bibliotekanauki.pl/articles/363070.pdf
- Data publikacji:
- 2011
- Wydawca:
- Uniwersytet Warmińsko-Mazurski w Olsztynie
- Tematy:
-
połączone analizy bioefektywne
derywatyzacja
HPTLC
liniowy zakres kalibracji
kwantyfikacja
TLC
herbicydy triazynowe
video densytometria
bioeffective-linked analysis
derivatization
linear calibration range
quantification
triazine herbicides
videodensitometry - Opis:
- We present a video-densitometric quantification method for the triazine herbicides atraton, terbumeton, simazine, atrazine and terbutylazine. Triazine herbicides were separated on silica gel using methyl-t-butyl ether, cyclohexane (1+1, v/v) as mobile phase. The quantification was based on a bioeffective-linked analysis using chloroplast and 2,6-dichlorophenolindophenol. Within 1-2 minutes HILL-reaction inhibitor substances show blue-grey zones on a pale yellowgreen background. To increase the contrast, the moist plate can be dipped into a solution of PEG-600 (10% PEG-600 in methanol) for 2s. Measurements were carried out using a 16 bit ST-1603ME CCD camera with 1.56 megapixels (from Santa Barbara Instrument Group, Inc., Santa Barbara, USA). A white LED was used for illumination purposes. The range of linearity covers more than one magnitude using the (1/R) - 1 expression data transformation. The method can be used for herbicide screenings in environmental samples, because not spectral sensitivity but herbicide activity is measured. The separation method is cheap, fast and reliable.
- Źródło:
-
Environmental Biotechnology; 2011, 7, 2; 47-52
1734-4964 - Pojawia się w:
- Environmental Biotechnology
- Dostawca treści:
- Biblioteka Nauki
Artykuł