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Wyszukujesz frazę "Dąbrowska, Anna" wg kryterium: Autor

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    Wyświetlanie 1-6 z 6
    Tytuł:
    The use of a one-step PCR method for the identification of Microsporum canis and Trichophyton mentagrophytes infection of pets
    Autorzy:
    Dąbrowska, Iwona
    Dworecka-Kaszak, Bożena
    Brillowska-Dąbrowska, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1039306.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    dermatophytosis
    animals
    pan-dermatophytes
    PCR
    Opis:
    Introduction: Dermatophytes are a closely related group of keratinophilic fungi. They encompass important etiological agents of superficial fungal infections. These fungi are able to invade keratinized tissues of humans and animals, causing dermatophytosis (ringworm) of hair, nails or skin. The aim: Traditional diagnostics of ringworm is based on morphological identification of cultured fungi and is time-consuming. Materials and methods: In this study, we applied a method patented by Brillowska-Dabrowska and coworkers (Brillowska-Dąbrowska A, Saunte DM, Arenderup MC, 2007, Five-hour diagnosis of dermatophyte nail infections with specific detection of Trichophyton rubrum. J Clin Microbiol 45: 1200-1204) which involves extraction of fungal DNA and PCR amplification with pan-dermatophyte primers to confirm the presence of dermatophytes. Results: The method used here is able to confirm the presence of dermatophyte DNA in pure cultures in less than 5 hours.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 2; 375-378
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Real-time PCR approach in dermatophyte detection and Trichophyton rubrum identification
    Autorzy:
    Kobylak, Natalia
    Bykowska, Barbara
    Nowicki, Roman
    Brillowska-Dąbrowska, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1039146.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    fungal infections
    dermatophytosis
    DNA extraction
    real-time PCR
    Opis:
    Dermatophytes are keratinophilic molds that infect human hair, nails and skin. Diagnosis of dermatophytosis is based on morphological, serological and biochemical features. However, identification is difficult and laborious due to similarities between microorganisms. Thus, there is considerable interest to develop mycological diagnostic procedures based on molecular biology methods. In this study, fast, two-step DNA extraction method and real-time PCR was used for detection of dermatophytes DNA using pan-dermatophyte primers and identification of Trichophyton rubrum from pure cultures. The applied method allowed correct detection of all dermatophytes and correct identification of Trichophyton rubrum in less than 2 hours.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 1; 119-122
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    PCR and real-time PCR assays to detect fungi of Alternaria alternata species
    Autorzy:
    Kordalewska, Milena
    Brillowska-Dąbrowska, Anna
    Jagielski, Tomasz
    Dworecka-Kaszak, Bożena
    Powiązania:
    https://bibliotekanauki.pl/articles/1038891.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Alternaria alternata
    detection
    identification
    PCR
    real-time PCR
    Opis:
    Fungi of the Alternaria genus are mostly associated with allergic diseases. However, with a growing number of immunocompromised patients, these fungi, with A. alternata being the most prevalent one, are increasingly recognized as etiological agents of infections (phaeohyphomycoses) in humans. Nowadays, identification of Alternaria spp. requires their pure culture and is solely based on morphological criteria. Clinically, Alternaria infections may be indistinguishable from other fungal diseases. Therefore, a diagnostic result is often delayed or even not achieved at all. In this paper we present easy to perform and interpret PCR and real-time PCR assays enabling detection of A. alternata species. On the basis of alignment of β-tubulin gene sequences, A. alternata-specific primers were designed. DNA from fungal isolates, extracted in a two-step procedure, were used in PCR and real-time PCR assays followed by electrophoresis or melting temperature analysis, respectively. The assays specificity was confirmed, since positive results were obtained for all A. alternata isolates, and no positive results were obtained neither for other molds, dermatophytes, yeast-like fungi, nor human DNA. The assays developed here enable fast and unambiguous identification of A. alternata pathogens.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 4; 707-712
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Examination of cyp51A and cyp51B expression level of the first Polish azole resistant clinical Aspergillus fumigatus isolate
    Autorzy:
    Brillowska-Dąbrowska, Anna
    Mroczyńska, Martyna
    Nawrot, Urszula
    Włodarczyk, Katarzyna
    Kurzyk, Ewelina
    Powiązania:
    https://bibliotekanauki.pl/articles/1038929.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Aspergillus fumigatus
    azole resistance
    cyp51A
    cyp51B
    Opis:
    Aspergillus fumigatus is one of the most prevalent airborne fungal pathogens causing infections worldwide. Most A. fumigatus strains are susceptible to azoles, which are administered as the first line therapeutics. However, during last decade the acquired resistance to triazoles by these species has been described. There is a number of publications concerning the examination of clinical A. fumigatus strains from different countries, however there has been no report from Poland. Here, we describe for the first time, an examination of cyp51A and cyp51B expression level of 11 clinical A. fumigatus strains isolated during 2007-2014 period from the collection of Medical University in Wrocław. Their susceptibility to itraconazole, voriconazole and posaconazole has been examined. The MIC values of triazoles for one of the examined isolates were respectively: > 8 mg/L for itraconazole, 2 mg/L for voriconazole and 0.5 mg/L for posaconazole. The cyp51A gene with its promoter region of all isolates was sequenced. It was found that the resistant isolate harbors the TR34/L98H mutation in the cyp51A gene and when cultured on media supplemented with voriconazole exhibits overexpression of both, cyp51A and cyp51B genes. The level of cyp51A gene expression was about 50 times higher than cyp51B.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 4; 837-839
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Removal of cyclohexane and ethanol from air in biotrickling filters inoculated with Candida albicans and Candida subhashii
    Usuwanie z powietrza cykloheksanu i etanolu w biofiltrach strużkowych zasiedlonych grzybami Candida albicans i Candida subhashii
    Autorzy:
    Rybarczyk, Piotr
    Marycz, Milena
    Szulczyński, Bartosz
    Brillowska-Dąbrowska, Anna
    Rybarczyk, Agnieszka
    Gębicki, Jacek
    Powiązania:
    https://bibliotekanauki.pl/articles/1845437.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    biofilm
    flow cytometry
    removal efficiency
    ethanol
    biofiltration
    cyclohexane
    cytometria przepływowa
    skuteczność usuwania
    etanol
    biofiltracja
    cykloheksan
    Opis:
    This paper presents investigations on the removal of cyclohexane and ethanol from air in polyurethane- -packed biotrickling filters, inoculated with Candida albicans and Candida subhashii fungal species. Results on process performance together with flow cytometry analyses of the biofilm formed over packing elements are presented and discussed. The results indicate that the presence of ethanol enhances the removal efficiency of cyclohexane from air. This synergistic effect may be attributed to both co-metabolism of cyclohexane with ethanol as well as increased sorption efficiency of cyclohexane to mineral salt medium in the presence of ethanol. Maximum elimination capacities of 89 g m-3 h-1 and 36.7 g m-3 h-1 were noted for cyclohexane and ethanol, respectively, when a mixture of these compounds was treated in a biofilter inoculated with C. subhashii. Results of flow cytometry analyses after 100 days of biofiltration revealed that about 91% and 88% of cells in biofilm remained actively dividing, respectively for C. albicans and C. subhashii species, indicating their good condition and ability to utilize cyclohexane and ethanol as a carbon source.
    W pracy przedstawiono badania nad usuwaniem cykloheksanu i etanolu z powietrza w boifiltrach zraszanych, wypełnionych pianką poliuretanową, zasiedloną grzybami z gatunku Candida albicans i Candida subhashii. Przedstawiono i omówiono wyniki dotyczące wydajności procesu (na podstawie pomiarów techniką chromatografii gazowej) wraz z wynikami cytometrii przepływowej dla utworzonego biofilmu. Uzyskano wartości zdolności usuwania, wynoszące około 89 g m-3 h-1 i 36.7 g m-3 h-1, odpowiednio dla cykloheksanu i etanolu, gdy te związki jednocześnie poddawano procesowi biofiltracji w biofiltrze zaszczepionym Candida subhashii. Wyniki wskazują, że obecność etanolu powoduje zwiększenie skuteczności usuwania cykloheksanu z powietrza. Wzrost skuteczności usuwania z powietrza cykloheksanu w obecności etanolu może wynikać z polepszonego metabolizmu cykloheksanu w takich warunkach oraz z ograniczenia bariery dla przenikania masy, wskutek lepszych właściwości sorpcyjnych cieczy zraszającej wobec cykloheksanu w obecności etanolu.
    Źródło:
    Archives of Environmental Protection; 2021, 47, 1; 26-34
    2083-4772
    2083-4810
    Pojawia się w:
    Archives of Environmental Protection
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Relation of the polymorphism of cyp51A sequence and the susceptibility of Aspergillus fumigatus isolates to triazoles determined by commercial gradient test (Etest) and by reference methods
    Autorzy:
    Nawrot, Urszula
    Sulik-Tyszka, Beata
    Kurzyk, Ewelina
    Mroczyńska, Martyna
    Włodarczyk, Katarzyna
    Wróblewska, Marta
    Basak, Grzegorz
    Brillowska-Dąbrowska, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1038548.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Aspergillus fumigatus
    triazole resistance
    susceptibility testing
    Etest
    cyp51A sequence
    Opis:
    The aim of this study was to evaluate the accuracy of commercial gradient test (Etest) in the detection of triazole resistant Aspergillus fumigatus isolates using reference microdilution methods and the analysis of sequences of the cyp 51A gene. The study was performed on twenty clinical isolates which were identified as Aspergillus fumigatus based on the DNA sequences of the ITS1-2 fragment of ribosomal DNA and the β-tubulin gene, out of them seventeen isolates showed wild-type cyp51A sequence and three were positive for the mutation TR34/L98H. All isolates were tested for the susceptibility to itraconazole (ITZ), voriconazole (VOR) and posaconasole (POS) using microdilution methods, according to EUCAST and CLSI protocols, as well as using Etest. The results of microdilution and Etests were analysed separately according to clinical breakpoints (CBP) defined by EUCAST version 7.0 and epidemiological cut off values (ECV). Etest as well as reference methods excellently recognised the WT isolates, which were susceptible to all tested triazoles, regardless of the method and CBP or ECV criteria used. The Etest recognized three non-WT isolates as resistant or intermediately sensitive to ITZ and POS and one as resistant to VOR. The categorical concordance between Etests and EUCAST and Etests and the CLSI method ranged from 90 to 100%. The interpretation of the results obtained from routine A. fumigatus Etests requires great caution. The use of the confirmative examinations with reference AST methods as well as with molecular tests is recommended.
    Źródło:
    Acta Biochimica Polonica; 2017, 64, 4; 631-634
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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