- Tytuł:
- Correlation of chromosome damage and promoter methylation status of the DNA repair genes MGMT and hMLH1 in Chinese vinyl chloride monomer (VCM)-exposed workers
- Autorzy:
-
Wu, Fen
Liu, Jing
Qiu, Yu-Lan
Wang, Wei
Zhu, Shou-Min
Sun, Pin
Miao, Wen-Bin
Li, Yong-Liang
Brandt-Rauf, Paul W.
Xia, Zhao-Lin - Powiązania:
- https://bibliotekanauki.pl/articles/2179802.pdf
- Data publikacji:
- 2013-03-01
- Wydawca:
- Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
- Tematy:
-
vinyl chloride monomer
chromosome damage
MGMT
hMLH1
DNA methylation - Opis:
- Objective: To explore the association of the methylation status of MGMT and hMLH1 with chromosome damage induced by vinyl chloride monomer (VCM). Materials and Methods: Methylation of MGMT and hMLH1 was measured in 101 VCM-exposed workers by methylation-specifi c PCR. Chromosome damage in peripheral blood lymphocytes was measured by the cytokinesis-block micronucleus assay. The subjects were divided into chromosome damaged and non-damaged groups based on the normal reference value of micronuclei frequencies determined for two control groups. Results: MGMT promoter methylation was detectable in 5 out of 49 chromosome damaged subjects, but not in the chromosome non-damaged subjects; there was a signifi cant difference in MGMT methylation between the two groups (p < 0.05). Conclusions: We detected aberrant promoter methylation of MGMT in a small number of chromosome damaged VCM-exposed workers, but not in the chromosome non-damaged subjects. This preliminary observation warrants further investigation in a larger study.
- Źródło:
-
International Journal of Occupational Medicine and Environmental Health; 2013, 26, 1; 173-182
1232-1087
1896-494X - Pojawia się w:
- International Journal of Occupational Medicine and Environmental Health
- Dostawca treści:
- Biblioteka Nauki
Artykuł