- Tytuł:
- Exogenous steroid hormones stimulate full development of autonomous endosperm in Arabidopsis thaliana
- Autorzy:
-
Rojek, J.
Pawelko, L.
Kapusta, M.
Naczk, A.
Bohdanowicz, J. - Powiązania:
- https://bibliotekanauki.pl/articles/59176.pdf
- Data publikacji:
- 2015
- Wydawca:
- Polskie Towarzystwo Botaniczne
- Tematy:
-
steroid hormone
endosperm
Arabidopsis thaliana
apomixis
DNA methylation - Opis:
- Most flowering plants, including important crops, require double fertilization to form an embryo and endosperm, which nourishes it. Independence from fertilization is a feature of apomictic plants that produce seeds, from which the plants that are clones of the mother plant arise. The phenomenon of apomixis occurs in some sexual plants under specific circumstances. Since the launch of a fertilization-independent mechanism is considered a useful tool for plant breeding, there have been efforts to artificially induce apomixis. We have been able to produce fertilization-independent endosperm in vitro in Arabidopsis over the last few years. This paper demonstrates the methods of improving the quality of the endosperm obtained using plant and mammalian steroid hormones. Additionally, it shows the study on the autonomous endosperm (AE) formation mechanism in vitro. This paper examines the effect of exogenous steroid hormones on unfertilized egg and central cell divisions in culture of unpollinated pistils of Arabidopsis Col-0 wild-type andfie-1 mutant. All media with hormones used (estrone, androsterone, progesterone, and epibrassinolide) stimulated central cell divisions and fertilization-independent endosperm development. The stages of AE development followed the pattern of Arabidopsis thaliana wild type after fertilization. Subsequent stages of AE were observed from 2-nuclear up to cellular with the most advanced occurring on medium with 24-epibrassinolide and progesterone. The significant influence of mammalian sex hormones on speed of AE development and differentiation was noticed. Using restriction analysis, the changes in methylation of FIE gene was established under in vitro condition. The authors of this paper showed that Arabidopsis thaliana has a high potency to fertilization-independent development.
- Źródło:
-
Acta Societatis Botanicorum Poloniae; 2015, 84, 2
0001-6977
2083-9480 - Pojawia się w:
- Acta Societatis Botanicorum Poloniae
- Dostawca treści:
- Biblioteka Nauki
Artykuł