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    Wyświetlanie 1-6 z 6
    Tytuł:
    Microarrays - new possibilities for detecting biological factors hazardous for humans and animals, and for use in environmental protection
    Autorzy:
    Mirski, T.
    Bartoszcze, M.
    Bielawska-Drozd, A.
    Gryko, R.
    Kocik, J.
    Niemcewicz, M.
    Chomiczewski, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/51516.pdf
    Data publikacji:
    2016
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2016, 23, 1
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Q fever - selected issues
    Autorzy:
    Bielawska-Drozd, A.
    Cieslik, P.
    Mirski, T.
    Bartoszcze, M.
    Knap, J.P.
    Gawel, J.
    Zakowska, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/49820.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Q fever
    animal species
    man
    Coxiella burnetii
    intracellular parasite
    parasite
    htpAB gene
    IS1111 gene
    genome
    plasmid
    pathomechanism
    infection
    bioterrorism
    epidemiology
    epizootiology
    treatment
    prophylaxis
    laboratory diagnostics
    serological method
    molecular diagnostics
    Opis:
    Q fever is an infectious disease of humans and animals caused by Gram-negative coccobacillus Coxiella burnetii, belonging to the Legionellales order, Coxiellaceae family. The presented study compares selected features of the bacteria genome, including chromosome and plasmids QpH1, QpRS, QpDG and QpDV. The pathomechanism of infection – starting from internalization of the bacteria to its release from infected cell are thoroughly described. The drugs of choice for the treatment of acute Q fever are tetracyclines, macrolides and quinolones. Some other antimicrobials are also active against C. burnetii, namely, telitromycines and tigecyclines (glicylcycline). Q-VAX vaccine induces strong and long-term immunity in humans. Coxevac vaccine for goat and sheep can reduce the number of infections and abortions, as well as decrease the environmental transmission of the pathogen. Using the microarrays technique, about 50 proteins has been identified which could be used in the future for the production of vaccine against Q fever. The routine method of C. burnetii culture is proliferation within cell lines; however, an artificial culture medium has recently been developed. The growth of bacteria in a reduced oxygen (2.5%) atmosphere was obtained after just 6 days. In serology, using the IF method as positive titers, the IgM antibody level >1:64 and IgG antibody level >1:256 (against II phase antigens) has been considered. In molecular diagnostics of C. burnetii infection, the most frequently used method is PCR and its modifications; namely, nested PCR and real time PCR which detect target sequences, such as htpAB and IS1111, chromosome genes (com1), genes specific for different types of plasmids and transposase genes. Although Q fever was diagnosed in Poland in 1956, the data about the occurrence of the disease are incomplete. Comprehensive studies on the current status of Q fever in Poland, with special focus on pathogen reservoirs and vectors, the sources of infection and molecular characteristics of bacteria should be conducted.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Bacillus anthracis infections – new possibilities of treatment
    Autorzy:
    Zakowska, D.
    Bartoszcze, M.
    Niemcewicz, M.
    Bielawska-Drozd, A.
    Knap, J.
    Cieslik, P.
    Chomiczewski, K.
    Kocik, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51666.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2015, 22, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Review of methods used for identification of biothreat agents in environmental protection and human health aspects
    Autorzy:
    Mirski, T.
    Bartoszcze, M.
    Bielawska-Drozd, A.
    Cieslik, P.
    Michalski, A.J.
    Niemcewicz, M.
    Kocik, J.
    Chomiczewski, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/51351.pdf
    Data publikacji:
    2014
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2014, 21, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Severe Influenza Outbreak in Western Ukraine in 2009 – a molecular-epidemiological study
    Autorzy:
    Kocik, J.
    Niemcewicz, M.
    Johns, M.
    Jerke, K.
    Michalski, A.
    Bielecka, A.
    Lasocki, K.
    Gawel, J.
    Bielawska-Drozd, A.
    Joniec, J.
    Kolodziej, M.
    Graniak, G.
    Goniewicz, M.
    Kubiak, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/50555.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Opis:
    Introduction: In the autumn of 2009 the authors participated in a humanitarian operation in Western Ukraine by undertaking an epidemiological investigation of an influenza-like-illness (ILI) in the L’viv Oblast region. Mobile biological survey teams took samples from civilian patients with severe acute respiratory distress syndrome, rapid transportation of the samples, and their molecular analysis in Poland to provide accurate results. Objective: The aim of the study was the molecular and epidemiological analysis of the biological samples collected. Material and Methods: Real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR), multiplex PCR techniques, traditional Sanger Sequencing and classical viral culture methods were used. Results: Among the 124 influenza-like illness cases, ~50% (58) were positive for influenza A virus in WHO-CDC molecular assay, including subtyping. The specimens were further analyzed to confirm results and determine the genetic sequence. Phylogenetically, the nucleotide similarity of both the Ukraine specimens and reference A/California/7/2009 (pH1N1) was 99.2–99.3%. Oseltamivir resistance was not registered. HA1 region characterization showed an overall protein identity of 98.5–99.4%. Conclusions: An unexpected high contribution of influenza A was confirmed among ILI patients, as well as a very limited number of other detected viruses, indicate that the 2009 epidemic in western Ukraine was strongly related to novel influenza A/H1N1. The importance of swift sharing of information and reference laboratories networking in surveillance, as well as serving governments and international agencies in pursuing adequate actions, should be stressed.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 3
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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