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    Tytuł:
    The use of attractants in reducing the occurrence of the Prunus serotina (Ehrh.)
    Autorzy:
    Baranowska, M.
    Szczepaniak, O.
    Meres, B.
    Korzeniewicz, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/28407527.pdf
    Data publikacji:
    2023
    Wydawca:
    Instytut Badawczy Leśnictwa
    Źródło:
    Folia Forestalia Polonica. Series A . Forestry; 2023, 65, 2; 96-103
    0071-6677
    Pojawia się w:
    Folia Forestalia Polonica. Series A . Forestry
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zastosowanie technik biologii molekularnej do detekcji Erysiphe alphitoides (Griffon & Maubl.) U. Braun and S. Takam. w organach roślinnych
    Applying the molecular biology techniques to the detection of Erysiphe alphitoides (Griffon & Maubl.) U. Braun and S. Takam. in plant parts
    Autorzy:
    Roszak, R.
    Baranowska, M.
    Belka, M.
    Behnke-Borowczyk, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/979672.pdf
    Data publikacji:
    2019
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    drzewa lesne
    dab
    Quercus
    paki
    liscie
    pedy
    maczniak prawdziwy debu
    Erysiphe alphitoides
    wykrywanie
    techniki biologii molekularnej
    oak powdery mildew
    sessile oak
    overwintering
    fungal diseases
    its
    Opis:
    Erysiphe alphitoides causes the most common disease of assimilation apparatus of oaks of different age. It is believed that the pathogen overwinters in buds of the host plant or in the cracks of the bark. The aim of the study was to search for the presence of E. alphitoides in buds, leaves, and wood of sessile oak shoots using molecular techniques. Two hypotheses have been tested: (i) oaks are infected by E. alphitoides, and (ii) the pathogen overwinters in the host plant buds. The samples used in the study were collected form sessile oak trees (Miradz Forest District; 52°41'23.197'N, 18°25'33.942'E) in 2017, and consisted of dormant buds (collected in May), young leaves (collected in June), leaves with visible symptoms of the disease (collected in August), shoot with sip and surface layers of wood (collected in December). Additional part of buds was collected in May 2018. The PCR reaction was carried out with primers specific for E. alphitoides and E. hypophylla. For species identification Sanger method was used. The resulting sequences were compared using BLAST algorithm with reference sequences deposited in the NCBI database. Sequences from isolates obtained from leaves showed 97−99% similarity to the reference sequence of E. alphitoides. Pathogen did not occur in the superficial layers of shoots and buds. As some studies show, at very low temperatures (below –20°C), the mycelium of the pathogen dies, therefore further study should be undertaken on wintering of the pathogen causing the powdery mildew disease on oaks. It also should be examined whether the pathogen overwinters in the form of bagnial spores produced in chasmothecia overwintering on leaves, in the leaf buds, or in bark cracks, and whether the wintering site of the pathogen is related to the age of trees and or to the stands where the trees grow.
    Źródło:
    Sylwan; 2019, 163, 09; 740-745
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Pory roku a zbiorowisko grzybów zasiedlających pniaki czeremchy amerykańskiej
    Seasonal changes in fungi colonies inhabiting black cherry stumps
    Autorzy:
    Baranowska, M.
    Korzeniewicz, R.
    Kartawik, N.
    Behnke-Borowczyk, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/979585.pdf
    Data publikacji:
    2019
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    drzewa lesne
    gatunki inwazyjne
    czeremcha amerykanska
    Prunus serotina
    fitopatologia
    pniaki czeremchy
    grzyby zasiedlajace drewno
    grzyby mikroskopowe
    identyfikacja
    system Illumina
    pory roku
    illumina system
    invasive species
    dead wood
    saprotrophs
    wood−decay fungus
    Opis:
    Black cherry is an important invasive species in forest ecosystems in Poland. It developed mainly in Scots pine stands. The aim of the study was to identify microscopic fungi colonies inhabiting black cherry stumps. In the spring of 2017, wood samples were collected from stumps created after cutting in each subsequent month of 2016 in the Podanin Forest District (19°28'00'E, 52°04'00'N). Stumps up to 5 cm and more than 5 cm in diameter were selected for the study. In total, 72 discs from the first stage of the decomposition of wood were collected. The discs were drilled to obtain fine sawdust for further analysis. The trials were divided into four periods. DNA isolation was performed using the Plant Genomic DNA purification kit (ThermoScientific). The ITS1/2 rDNA region was used for species identification. The analysis was carried out using specific primers. The obtained product was purified and sequenced using the Illumina SBS technology. The resulting sequence was compared using the BLAST algorithm with reference sequences from the NCBI database. The communities of fungi inhabiting the black cherry stumps differed from each other. The highest number of taxa was identified in samples from stumps over 5 cm sheared in autumn and winter, while the lowest in stumps up to 5 cm sheared in spring and winter. Fungi belonging to the Ascomycota and Basidiomycota, Blastocladiomycota, Glomeromycota, Zygomycota and non−cultivable fungi and organisms belonging to other kingdoms were identified. Saprophytes from Menispora sp. dominated on the analysed stumps. The collection of fungi of larger stumps was more diverse and more numerous than on stumps with a smaller diameter. A greater diversity of taxa was distinguished by the stumps of tree fallen in the growing season. The majority of the analyzed samples were dominated by Ascomycota. Basidiomycota clusters dominated in the winter. The predominant share of saprotrophs shows the distribution of stumps. The most desirable effect of the research would be the indication of naturally occurring saprotroph, whose operation would reduce the black cherry’s yield strength. The applied method of sequencing based on the Illumina System was effective to determine the composition of the fungal population.
    Źródło:
    Sylwan; 2019, 163, 10; 872-880
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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