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    Wyświetlanie 1-9 z 9
    Tytuł:
    A comparison of the effectiveness of the microscopic method and the multiplex PCR method in identifying and discriminating the species of Nosema spp. spores in worker bees (Apis mellifera)from winter hive debris
    Autorzy:
    Michalczyk, M.
    Sokol, R.
    Szczerba-Turek, A.
    Bancerz-Kisiel, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/32256.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The objective of this study was to compare the effectiveness of the multiplex PCR method and traditional light microscopy in identifying and discriminating the species of Nosema spp. spores in worker bees from winter hive debris in the Province of Warmia and Mazury (NE Poland). A total of 1000 beesdead after from the bottom of the hive from bee colonies were analyzed. Spores were identified with the use of a light microscope (400-600x magnification). Spores were assigned to species by the multiplex PCR method. The microscopic evaluation revealed the presence of Nosema spp. spores in 803 samples (80.3%). Nosema ceranae spores were observed in 353 positive samples (43.96%), Nosema apis spores were found in 300 samples (37.35%), while 150 samples (19.67%) showed signs of a mixed infection. A multiplex PCR analysis revealed that 806 samples were infested with Nosema spp., of which 206 were affected only by Nosema ceranae, 600 showed signs of mixed invasion, while no samples were infected solely by Nosema apis parasites. In two cases, the presence of spores detected under a light microscope was not confirmed by the PCR analysis. The results of the study indicate that Nosema ceranae is the predominant parasitic species found in post-winter worker bees from the bottom of the hive in the region of Warmia and Mazury.
    Źródło:
    Polish Journal of Veterinary Sciences; 2011, 14, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Sequence variants of Yersinia enterocolitica ystB gene detected in wild animals in Poland
    Autorzy:
    Pieczywek, M.
    Bancerz-Kisiel, A.
    Szczerba-Turek, A.
    Szweda, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087747.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Yersinia enterocolitica
    ystB gene
    phylogenetic analysis
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 2; 397-399
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Distribution of the ymoA and ystA genes and enterotoxins Yst production by Yersinia enterocolitica strains isolated from humans and pigs
    Autorzy:
    Bancerz-Kisiel, A.
    Szczerba-Turek, A.
    Platt-Samoraj, A.
    Szweda, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/30931.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Yersinia (Y.) enterocolitica is the third etiological agent of human diarrhea in terms of the number of confirmed clinical cases. One of the important virulence markers is the yst gene which encodes the production of enterotoxins Yst (Yersinia stable toxins). However, not all strains with yst genes produce enterotoxins, what seems to be caused by the ymoA gene encoding the production of the YmoA protein inhibiting the expression of various genes. The purpose of our study was to evaluate the distribution of the ymoA and ystA genes and Yst production by Y. enterocolitica isolated from humans and pigs. All the studied strains obtained from pigs had the ystA gene which indicates that they belong to the group of strains commonly regarded as pathogenic, but the ability to produce YstA was detected in only 14 out of 96 examined strains. The fragments of ystA gene were also detected in all Y.enterocolitica strains isolated from human cases of diarrhea. Amplification of a fragment of the ymoA gene was detected in all the studied strains, both from humans and pigs, based on the presence of a 330 bp band. Thus no correlation was identified between the occurrence of the ymoA and ystA genes and the production of a specific type of enterotoxin.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Phylogenetic analysis of bovine papillomavirus E5 detected in equine sarcoids in Poland
    Autorzy:
    Szczerba-Turek, A.
    Siemionek, J.
    Bancerz-Kisiel, A.
    Ras, A.
    Szweda, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/30859.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The aim of the study was to analyse a part of the sequence of the E5 gene of bovine papil-lomaviruses (BPV) associated with equine sarcoids in Polish horses. Samples of 40 skin lesions obtained from 29 horses were collected for molecular examination. The PCR amplicons of BPV DNA were detected in 38 specimens. After phylogenetic analysis 37 specimens were recognized as BPV-1 and one as BPV-2. Phylogenetic analysis has allowed the classification of the amplicons into two phylogenetic groups (A1,) and four separate isolates (2, 10, 16, 17).
    Źródło:
    Polish Journal of Veterinary Sciences; 2011, 14, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Bioserotypes and virulence markers of Y. enterocolitica strains isolated from roe deer (Capreolus capreolus) and red deer (Cervus elaphus)
    Autorzy:
    Bancerz-Kisiel, A.
    Szczerba-Turek, A.
    Platt-Samoraj, A.
    Socha, P.
    Szweda, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087938.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Free-living animals are an important environmental reservoir of pathogens dangerous for other animal species and humans. One of those is Yersinia (Y.) enterocolitica, the causative agent of yersiniosis - foodborne, enzootic disease, significant for public health. The purpose of the study was to identify bioserotypes and virulence markers of Y. enterocolitica strains isolated from roe deer (Capreolus capreolus) and red deer (Cervus elaphus) obtained during the 2010/2011 hunting season in north-eastern Poland. From among 48 rectal swabs obtained from 24 roe deer, two strains of Y. enterocolitica from one animal were isolated. Although both belonged to biotype 1A they were identified as different serotypes. The strain obtained from cold culture (PSB) belonged to serotype O:5, while the strain isolated from warm culture (ITC) was regarded as nonidentified (NI), what may suggest mixed infection in that animal. The presence of ystB gene, coding for YstB enterotoxin, directly related to Y. enterocolitica pathogenicity was detected in both strains using triplex PCR. The effect of the examination of 32 swabs obtained from 16 red deer was the isolation of two Y. enterocolitica strains from two different animals. Both belonged to biotype 1A with NI serotype, but were originated from different types of culture. They gave positive results in case of products of a size corresponding to the ystB gene. No amplicons corresponding to ail and ystA genes were found. Roe deer and red deer may carry and shed Y. enterocolitica, what seems to be important in aspect of an environmental reservoir of this pathogen. The Y. enterocolitica strains isolated from wild ruminants had the amplicons of the ystB gene, what suggest they can be potential source of Y. enterocolitica infection for humans.
    Źródło:
    Polish Journal of Veterinary Sciences; 2014, 17, 2; 315-319
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Yersinia enterocolitica strains isolated from beavers (Castor fiber)
    Autorzy:
    Platt-Samoraj, A.
    Syczylo, K.
    Bancerz-Kisiel, A.
    Szczerba-Turek, A.
    Gizejewska, A.
    Szweda, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/31544.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Pseudocloacal swabs and palatine tonsils from beavers have been examined for the Yersinia enterocolitica presence. Thirty-six samples from 24 beavers were collected and subjected to bacteriological examinations including sero- and biotypisation. Amplicons confirmed by PCR as Y. enterocolitica were sequenced. Positive samples originated from 4 out of the 24 beavers (16.7 %) and all the strains belonged to biotype 1A. The study suggested that Y. enterocolitica could be isolated from beavers, which may therefore be treated as a reservoir, a significant factor of water contamination and a vector of the Y. enterocolitica.
    Źródło:
    Polish Journal of Veterinary Sciences; 2015, 18, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Quantitative PCR High-Resolution Melting (qPCR-HRM) curve analysis, a new approach to rapid detection and differentiation of bovine papillomavirus detected in equine sarcoids
    Autorzy:
    Szczerba-Turek, A.
    Bancerz-Kisiel, A.
    Lipczynska, K.
    Siemionek, J.
    Ras, A.
    Platt-Samoraj, A.
    Szweda, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087954.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The aim of the study was to evaluate a novel diagnostic scheme which combines quantitative PCR and High-Resolution Melting (qPCR-HRM) curve analysis for rapid differentiation based on E5 partial CDS of bovine papillomavirus type 1 or 2 (BPV-1 or BPV-2), and to perform a phylogenetic analysis of the complete CDS of the E5 gene of BPV detected in equine sarcoids. Samples of 38 skin lesions obtained from 27 horses were collected for molecular examinations. All lesions were clinically diagnosed as sarcoids, but results of histopathological examinations did not always corroborate the clinical diagnosis. Although all the samples were positive for the presence of BPV DNA, after qPCR-HRM analysis 6 (16%) specimens were recognized as BPV-1 “wild”, 24 (63%) as BPV-1 “European” and 8 (21%) as a “variant” of BPV E5 ORF partial CDS. Phylogenetic analysis based on nucleotide sequences of E2 ORF partial CDS and E5 ORF complete CDS was conducted on 7 specimens, whose sequences were published in GenBank and recognized as: 2PL (Accession Number - Acc. No. KC684939) - “variant” BPV-1, 7aPL (Acc. No. KC684940) - “European” BPV-1, 10PL (Acc. No. KC693480) - “variant” BPV-1, 16PL (Acc. No. KC693484) - “variant” BPV-2, 17PL (Acc. No. KC693481) - “variant” BPV-1, 20aPL (Acc. No. KC693482) - “European” BPV-1 and 20cPL (Acc. No. KC693483) - “wild” BPV-1. Amino acid (aa) sequences of E5 ORF complete CDS were also analyzed. The E5 variant of aa sequences found in isolate 10PL (protein identification - ID: AGM 20700) is a novel variant of E5 ORF complete CDS of BPV-1 detected in equine sarcoid in Poland.
    Źródło:
    Polish Journal of Veterinary Sciences; 2014, 17, 2; 239-245
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic evaluation of bovine papillomavirus types detected in equine sarcoids in Poland
    Autorzy:
    Szczerba-Turek, A.
    Siemionek, J.
    Ras, A.
    Bancerz-Kisiel, A.
    Platt-Samoraj, A.
    Lipczynska-Ilczuk, K.
    Szweda, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087581.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    bovine papillomavirus
    equine sarcoid
    disease ecology
    FAP59/FAP64
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 1; 25-29
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Monitoring of Yersinia enterocolitica strains from free - living animals using different methods
    Autorzy:
    Syczylo, K.
    Platt-Samoraj, A.
    Bancerz-Kisiel, A.
    Szczerba-Turek, A.
    Lipczynska, K.
    Jablonski, A.
    Procajlo, Z.
    Szweda, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/30966.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The aim of the study was to monitor Y. enterocolitica strains from free-living animals captured during 2011-2014 hunting seasons in Poland using warm (ITC) and cold (PSB) enrichment and molecular examination. Over 1600 samples have been cultured. After ITC/PSB enrichment 237 strains presenting features characteristic for Y. enterocolitica were isolated. Molecular examination using multiplex PCR revealed 140 isolates from PSB and 78 from ITC. The concentration of pathogenic Yersinia in asymptomatic carriers is low and the PCR detection should be preceded by bacteriological examination.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-9 z 9

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