- Tytuł:
- Development of 111In-DTPA-human polyclonal antibody complex for long-term inflammation/infection detection
- Autorzy:
-
Jalilian, A.
Rowshanfarzad, P.
Shafaii, K.
Kamali-Dehghan, M.
Moafian, J.
Akhlaghi, M.
Babaii, M.
Rajabifar, S.
Mirzaii, M. - Powiązania:
- https://bibliotekanauki.pl/articles/148870.pdf
- Data publikacji:
- 2005
- Wydawca:
- Instytut Chemii i Techniki Jądrowej
- Tematy:
-
radiopharmaceuticals
indium-111
human polyclonal antibody
inflammation
turpentine oil - Opis:
- Human polyclonal antibody (HIgG) was successively labeled with 111In-indium chloride after residulation with freshly prepared cyclic DTPA-dianhydride. The best results of the conjugation were obtained by the addition of solid DTPA-dianhydride (0.1 0.3 mg) to 100 mi l of the HIgG solution (0.2 0.4 mg/ml) at pH = 6 in phosphate buffer media at 25°C with continuous stirring for 30 min. Radio-thin-layer chromatography showed an overall radiochemical yield of 96 99% at optimized conditions (specific activity = 300 500 MBq/mg, radiochemical purity >98%). The final isotonic 111In-DTPA-HIgG complex was checked by radio-TLC to ensure the formation of only one species followed by filtration through a 0.22 mi filter. Preliminary long-term in vivo studies in turpentine-oil induced inflammation in rat model was performed to determine late complex distribution of the radioimmunoconjugate. The target/skin and target/ blood ratios were 27 and 51 after 24 h, and 23 and 51 after 110 h, showing a high selectivity of the radiopharmaceutical for inflammatory lesions.
- Źródło:
-
Nukleonika; 2005, 50, 3; 91-96
0029-5922
1508-5791 - Pojawia się w:
- Nukleonika
- Dostawca treści:
- Biblioteka Nauki
Artykuł