- Tytuł:
- High efficiency method to obtain supercoiled DNA with a commercial plasmid purification kit
- Autorzy:
-
Carbone, Antonietta
Fioretti, Flavia
Fucci, Laura
Ausió, Juan
Piscopo, Marina - Powiązania:
- https://bibliotekanauki.pl/articles/1039748.pdf
- Data publikacji:
- 2012
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
midi preparation
supercoiled plasmid isoform
plasmid purification
Qiagen kit
Plasmid DNA - Opis:
- Supercoiled state corresponds to the active form for plasmid applications. The relaxed circular form of plasmids is often inactive or poorly active. To obtain significant amounts of almost fully supercoiled DNA, we modified the standard protocol of a commercially available Qiagen plasmid purification kit. Our changes led to isolation of almost 100% of the plasmids in the supercoiled state. The modified protocol was used to purify different plasmids with consistent results. The purified plasmids maintain supercoiled state for about two months. The modified protocol is very advantageous because it allows easy DNA production with high degree of supercoiled form at low cost.
- Źródło:
-
Acta Biochimica Polonica; 2012, 59, 2; 275-278
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł