- Tytuł:
- Effects of pH on the activity and structure of choline oxidase from Alcaligenes species
- Autorzy:
-
Hekmat, Azadeh
Saboury, Ali
Moosavi-Movahedi, Ali
Ghourchian, Hedayatollah
Ahmad, Faizan - Powiązania:
- https://bibliotekanauki.pl/articles/1040713.pdf
- Data publikacji:
- 2008
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
optimum pH
choline oxidase
nile red
glycine-betaine - Opis:
- A reversible effect of pH on the ionization of amino-acid residues at the active center of choline oxidase was observed near the optimum pH (8). Inactivation of choline oxidase took place in the pH ranges 3-6 and 9-11, in which irreversible changes in the structure occur leading to the enzyme inactivation. The first order rate constants of the enzyme's inactivation at various pH values were estimated for the irreversible changes. The Arrhenius analysis revealed no significant changes in the activation enthalpy, while an increase in the activation entropy reflected an increase in the conformational freedom.
- Źródło:
-
Acta Biochimica Polonica; 2008, 55, 3; 549-557
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł