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    Wyświetlanie 1-4 z 4
    Tytuł:
    Content of glycogen and trehalose and activity of alpha-amylase and trehalase in Galleria mellonella larvae infected with entomopathogenic nematodes Steinernema affinis and S. feltiae
    Autorzy:
    Żółtowska, K.
    Łopieńska-Biernat, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/2144330.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Galleria mellonella
    Steinernema feltiae
    insect host
    amylase
    trehalase
    host
    parasite
    trehalose
    glycogen
    parasitology
    alpha-amylase
    nematode
    larva
    Steinernema affinis
    entomopathogenic nematode
    Opis:
    Introduction. The influence of infection with two species of entomopathogenic nematodes of Steinernematidae family on metabolism of glycogen and trehalose of the host was studied. Material and methods. Last instar larvae (L₇) of Galleria mellonella were experimentally infected with Steinernema affinis and S. feltiae. At 6, 12, 18 and 24 h after infection concentrations of trehalose and glycogen as well as activity of trehalase and α-amylase were determined. Results. The content of glycogen was lower in insects infected with S. feltiae than in the controls and animals infected with S. affinis. The content of trehalose was higher in insects from both infected groups than in the controls. Its concentration was slightly higher in larvae infected with S. affinis than in those infected with S. feltiae. The activity of α-amylase after infection with S. affinis was low. It was significantly higher in insects infected with S. feltiae. In animals of both infected groups, following a significant reduction at 6 h, the activity of trehalase remained at a similar level, higher than in the controls. In the paper the effects of infection with (i) different species of entomopathogenic nematodes and (ii) the importance of the developmental stage of the insect-host for changes in its metabolism of glycogen and trehalose were discussed.
    Źródło:
    Wiadomości Parazytologiczne; 2006, 52, 2; 103-107
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Activity of superoxide dismutase in Galleria mellonella larvae infected with entomopathogenic nematodes Steinernema affinis and S. feltiae
    Autorzy:
    Żółtowska, K.
    Grochla, P.
    Łopieńska-Biernat, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/2146340.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasitic disease
    superoxide dismutase
    Galleria mellonella
    Steinernema feltiae
    oxidative stress
    animal disease
    invasive disease
    parasite
    activity
    parasitology
    nematode
    Steinernema affinis
    larva
    entomopathogenic nematode
    Opis:
    Background. The influence of infection with two species of entomopathogenic nematodes of Steinernematidae family on the activity of superoxide dismutase (SOD) of the host was studied. Material and methods. Last instar larvae of Galleria mellonella were experimentally infected with Steinernema affinis and S. feltiae at 20 invasive juveniles per insect. At 6, 12, 18, 24 and 36 h after infection activity of SOD was determined in extracts from infected and control insects. Results. The activity of SOD decreased gradually in the controls during the experiment. The activity of enzyme was 2-4-times higher in insects from both infected groups than in the control. During the first 12 h of infection the activity of SOD in insects infected with S. feltiae was higher than in those infected with S. affinis, then the activity of enzyme in the insects of both infected groups stayed at a similar level. A significant decrease of SOD activity in infected was recorded in second day of the infection.
    Źródło:
    Wiadomości Parazytologiczne; 2006, 52, 4; 283-286
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The activity of hydrolases of larval stages of Anisakis simplex [Nematoda]
    Autorzy:
    Łopieńska-Biernat, E.
    Żółtowska, K.
    Rokicki, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2147476.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    hydrolase
    hydrolase activity
    Nematoda
    larval stage
    Anisakis simplex
    Opis:
    Activity of hydrolases during the third and fourth larval stage of Anisakis simplex was identified by applying the API ZYM test method. In A. simplex larvae the activity of phosphatases was high, particularly that of acid phosphatase (40 nmol/mg-'). Among esterases lack of activity of lipase (Cj 4) is worth noticing while the activity of esterases (C4) and (Cg) was high. The activity of those later two enzymes was higher in L larvae than in Ly larvae. The highest activity in the subclass of glucosidases was recorded for B-fucosidase and N-acetyl-B-glucosaminidase. A higher activity in L3 larvae than in Ly larvae was recorded for: B-glucuronidase and N-acetyl-B-glucosaminidase (2-fold) and B-fucosidase (3-fold). Differently the activity of B-galactosidase and B-glucosidase was higher in L4 larvae than in L3 larvae. The tests did not show activity of o-galactosidase, 8-glucosidase and amannosidase on both larval forms.
    Źródło:
    Wiadomości Parazytologiczne; 2004, 50, 3; 503-507
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antyoksydanty Contracaecum rudolphii [Nematoda]
    Antioxidants of Contracaecum rudolphii [Nematoda]
    Autorzy:
    Żółtowska, K.
    Farjan, M.
    Łopieńska-Biernat, E.
    Rokicki, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143804.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Contracaecum rudolphii
    pasozyty przewodu pokarmowego
    nicienie zoladkowo-jelitowe
    parazytologia
    nicienie
    Anisakidae
    przeciwutleniacze
    Nematoda
    enzymy antyoksydacyjne
    Opis:
    Contracaecum rudolphii is the parasitic nematode of fish−eating birds. In the extracts from female, male and larvae L3 and L4 isolated from the alimentary tracts of black cormorants the activity of five antioxidant enzymes: superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione transferase (GST), glutathione reductase (GR), catalase (CAT) and the content of ascorbate and total antioxidative status (TAS) were determined. They can be put in order according to the activity growth: GPX, SOD, GST, CAT and GR. The activity of GPX were very low in the nematodes' extracts (1.23–7.67 µU/mg). CAT had higher activity (0.47–0.72 U/mg). The activity of GR was the highest (50.51–69.88 U/mg). SOD activity in the female was higher by ca. 50% than in the male while GST activity was at similar levels. GR and CAT activities were higher by ca. 30% in the male than in the female nematodes. GST and GPX activity and TAS in larvae L3 were significantly lower than in the adult nematodes or in L4 larvae. The activity of GPX, GR and CAT was lower in L4 larvae than in the adult male (p<0.05). The content of ascorbate was almost the same in all stages of parasite development (0.21–0.38 mg/g). The above results indicate differences in antioxidant systems related to both the sex and the developmental stage of C. rudolphii.
    Źródło:
    Wiadomości Parazytologiczne; 2008, 54, 3; 237-241
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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