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Wyświetlanie 1-15 z 38
Tytuł:
Badanie przydatnosci testu ELISA do wczesnego diagnozowania inwazji z rodzaju Trichinella u swin
The usefulness of ELISA test early serological detection of Trichinella spp. infection in pigs
Autorzy:
Bien, J
Powiązania:
https://bibliotekanauki.pl/articles/838974.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
diagnostyka wczesna
pasozyty zwierzat
trzoda chlewna
Trichinella spiralis
parazytologia
inwazja pasozytnicza
przydatnosc
test ELISA
Źródło:
Annals of Parasitology; 2007, 53, 2; 149-151
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ procedury otrzymywania antygenów ekskrecyjno-sekrecyjnych Trichinella spiralis na wynik testu ELISA u zarażonych świń
The influence of the procedure of excretory -secretory L1 Trichinella spiralis antigen preparation on the efficiency of an ELISA test in pigs
Autorzy:
Bień, J.
Powiązania:
https://bibliotekanauki.pl/articles/2144343.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
analiza statystyczna
diagnostyka weterynaryjna
pasozyty
nicienie
Warszawa konferencja
otrzymywanie
procedury
trzoda chlewna
zwierzeta chore
parazytologia
Trichinella spiralis
konferencje
antygeny
wartosc diagnostyczna
test ELISA
Opis:
Background. Trichinellosis is a parasitic zoonosis transmitted to humans by consumption of raw or undercooked meat from animals infected by worms of the Trichinella genus. Every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease in Poland. The usefulness of ELISA for anti-T. spiralis IgG detection in pigs is still limited by the nature of antigen. The objective in the present study was to compare the usefulness of excretory-secretory antigens of L1 T. spiralis for the serological detection of IgG antibodies in pigs. Material and methods. The antigens were prepared in different laboratories: Ag ES L1 T. spiralis (N) in Germany, Ag ES L1 T. spiralis (W) in Italy and Ag ES L1 T. spiralis in Poland. Conventional, Iberian pigs were infected with 200, 1000 and 20 000 muscle larvae of T. spiralis. Serum samples were obtained at 5 and 1 dbi (day before infection), and 5, 10, 15, 20, 25, 30, 40, 50, 60 dpi (day post infection) and screened for specific IgG antibodies to excretory-secretory L1 T. spiralis antigens. Serum samples were obtained from the EU project TRICHIPORSE. The cut-off value of ELISA was determined on serum samples from 248 Trichinella-free pigs from Poznań and Boża Wola, that were examined by artificial digestion. Results. In pigs infected with 200 L1 T. spiralis larvae, specific IgG were detectable from 50 dpi, when the Ag ES L1 T. spiralis (N) was used, whereas when Ag ES L1T. spiralis (W) and Ag ES L1 T. spiralis were used, the specific IgG were detectable from 40 dpi. In pigs infected with 1000 L1 T. spiralis larvae, specific IgG was observed from 30 dpi when Ag ES L1 T. spiralis (W) and Ag ES L1 T. spiralis were used, but when Ag ES L1 T. spiralis (N) was used specific IgG were detectable from 40 dpi. In the group infected with the highest dose of T. spiralis larvae, specific IgG were detectable from 30 dpi when Ag ES L1 T. spiralis (N) and Ag ES L1 T. spiralis (W) were used, whereas when Ag ES L1 T. spiralis was used specific IgG were detectable from 20 dpi. The results strongly indicated that in the examined pigs, the specific IgG response against T. spiralis infection is dose dependent. Furthermore, it was shown that the high infectious dose induced earlier increasing of specific IgG response. Statistical analysis revealed a significant positive correlation between OD values obtained in procedures based on the three antigens. The results were statistically repeatable for procedures and for single pigs (P<0.01).
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 219-230
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Efficacy of albendazole against Trichinella pseudospiralis and Trichinella spiralis in mice
Wplyw albendazolu na przebieg zakazenia Trichinella pseudospiralis i Trichinella spiralis u myszy
Autorzy:
Bany, J
Lach, J.
Golinska, Z.
Powiązania:
https://bibliotekanauki.pl/articles/838080.pdf
Data publikacji:
1992
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Trichinella pseudospiralis
zwierzeta doswiadczalne
Trichinella spiralis
parazytologia
albendazol
myszy
nicienie
pasozyty
inwazja pasozytnicza
Trichinellidae
Opis:
Albendazole (Smith Kline, Beecham) in a dose of 20 mg/kg/day was given to B6C3F₁ mice exposed to 300 larvae of T. pseudospiralis or T. spiralis. The drug was introduced on days 2, 3 and 4; or 4, 5 and 6; or 18, 19 and 20 after infection. It limited the intensity of intestinal and muscle phases of trichinellosis. Given at the stage of maturation of adult forms and production of new-born larvae, the drug caused almost total elimination of both species from the intestines of mice and a decrease in the numbers of muscle larvae, with a tendency towards a greater reduction of larvae and adults of T. pseudospiralis. The drug did not change the course of infection when given at the phase of infectiveness of larvae.
Źródło:
Annals of Parasitology; 1992, 38, 3-4; 143-146
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wplyw procedury otrzymywania antygenow ekskrecyjno-sekrecyjnych Trichinella spiralis na wynik testu ELISA u zarazonych swin
The influence of the procedure of excretory -secretory L1 Trichinella spiralis antigen preparation on the efficiency of an ELISA test in pigs
Autorzy:
Bien, J
Powiązania:
https://bibliotekanauki.pl/articles/840241.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
analiza statystyczna
diagnostyka weterynaryjna
pasozyty
nicienie
Warszawa konferencja
otrzymywanie
procedury
trzoda chlewna
zwierzeta chore
parazytologia
Trichinella spiralis
konferencje
antygeny
wartosc diagnostyczna
test ELISA
Opis:
Background. Trichinellosis is a parasitic zoonosis transmitted to humans by consumption of raw or undercooked meat from animals infected by worms of the Trichinella genus. Every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease in Poland. The usefulness of ELISA for anti-T. spiralis IgG detection in pigs is still limited by the nature of antigen. The objective in the present study was to compare the usefulness of excretory-secretory antigens of L1 T. spiralis for the serological detection of IgG antibodies in pigs. Material and methods. The antigens were prepared in different laboratories: Ag ES L1 T. spiralis (N) in Germany, Ag ES L1 T. spiralis (W) in Italy and Ag ES L1 T. spiralis in Poland. Conventional, Iberian pigs were infected with 200, 1000 and 20 000 muscle larvae of T. spiralis. Serum samples were obtained at 5 and 1 dbi (day before infection), and 5, 10, 15, 20, 25, 30, 40, 50, 60 dpi (day post infection) and screened for specific IgG antibodies to excretory-secretory L1 T. spiralis antigens. Serum samples were obtained from the EU project TRICHIPORSE. The cut-off value of ELISA was determined on serum samples from 248 Trichinella-free pigs from Poznań and Boża Wola, that were examined by artificial digestion. Results. In pigs infected with 200 L1 T. spiralis larvae, specific IgG were detectable from 50 dpi, when the Ag ES L1 T. spiralis (N) was used, whereas when Ag ES L1T. spiralis (W) and Ag ES L1 T. spiralis were used, the specific IgG were detectable from 40 dpi. In pigs infected with 1000 L1 T. spiralis larvae, specific IgG was observed from 30 dpi when Ag ES L1 T. spiralis (W) and Ag ES L1 T. spiralis were used, but when Ag ES L1 T. spiralis (N) was used specific IgG were detectable from 40 dpi. In the group infected with the highest dose of T. spiralis larvae, specific IgG were detectable from 30 dpi when Ag ES L1 T. spiralis (N) and Ag ES L1 T. spiralis (W) were used, whereas when Ag ES L1 T. spiralis was used specific IgG were detectable from 20 dpi. The results strongly indicated that in the examined pigs, the specific IgG response against T. spiralis infection is dose dependent. Furthermore, it was shown that the high infectious dose induced earlier increasing of specific IgG response. Statistical analysis revealed a significant positive correlation between OD values obtained in procedures based on the three antigens. The results were statistically repeatable for procedures and for single pigs (P<0.01).
Źródło:
Annals of Parasitology; 2006, 52, 3; 219-230
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of Trichinella invasion on mice peritoneal macrophage migration inhibition
Autorzy:
Szkudlinski, J.
Powiązania:
https://bibliotekanauki.pl/articles/841166.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Trichinella pseudospiralis
migration
mouse
mice
Trichinella
macrophage
invasion
Trichinella spiralis
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Badanie przydatności testu ELISA do wczesnego diagnozowania inwazji z rodzaju Trichinella u świń
The usefulness of ELISA test early serological detection of Trichinella spp. infection in pigs
Autorzy:
Bień, J.
Powiązania:
https://bibliotekanauki.pl/articles/2143857.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
diagnostyka wczesna
pasozyty zwierzat
trzoda chlewna
Trichinella spiralis
parazytologia
inwazja pasozytnicza
przydatnosc
test ELISA
Opis:
Trichinellosis is a parasitic zoonosis transmitted to humans through consumption of raw or undercooked meat from animals infected with nematodes of the Trichinella genus. Every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease. The first step of the study was the optimization of a new ELISA method enabling an early and specific serological diagnosis of trichinellosis in pigs and wild boars using excretory−secretory (ES) antigens obtained from in vitro cultures of L1 T. spiralis. Serum samples were assayed for anti−T. spiralis IgG antibodies using the new ELISA protocol and a reference test — Standard manufactured by Institut Pourquier. The optimization involved the selection of suitable plates for antigen coating, dilution of sera and antibodies and their time of incubation. On the basis of the optimization a new ELISA procedure for the detection of IgG and IgM against T. spiralis was elaborated. Conventional, Iberian pigs and SPF (Specific Pathogen Free) pigs were infected with 200, 1000 and 20,000 muscle larvae of T. spiralis. Serum samples were obtained at 5 and 1 dbi (day before infection), and 5, 10, 15, 20, 25, 30, 40, 50, 60 dpi (day post infection) and screened for specific IgG antibodies against excretory−secretory L1 T. spiralis antigens. Serum samples were obtained from the EU project Trichiporse: „Safe pork and horse meat on EU markets: early and unbiased diagnostic tests for Trichinella”. Field samples of conventional pigs (1474) and wild boars (1784) were obtained from slaughter houses in different parts of Poland. Pigs were examined for the presence of Trichinella spp. using the artificial digestion method. Only four pigs were naturally infected with T. spiralis, the remaining were Trichinella larvae free. ELISA was used to examine IgG levels against L1 T. spiralis in pig and wild boar sera. The usefulness of ELISA for anti−IgG detection in pigs is usually limited by the nature of the antigen. The antigens were prepared in different laboratories: in Germany — Ag ES L1 T. spiralis (N), Italy — Ag ES L1 T. spiralis (W)) and in Poland — Ag ES L1 T. spiralis. Cut−off values for ELISA along with the estimated sensitivity and specificity were calculated using different methods: S/P%, M+3SD and ROC (Receiver Operating Characteristic). In SPF and Iberian pigs inoculated with 200, 1000 and 20,000 L1 T. spiralis, specific antibodies were detected 40, 30 and 25 dpi, respectively, with the use of the Standard (reference test). The analysis of the two ELISA procedures demonstrated a high sensitivity and specificity for the newly elaborated test utilizing the Ag ES L1 T. spiralis. In conventional pigs infected with 20,000 L1 T. spiralis specific antibodies were detected from 20 dpi when employing the new protocol. Similar results for the Standard and new ELISA test were obtained for serum samples of conventional pigs infected with 200 and 1000 larvae, which became positive from 40 dpi and 30 dpi, respectively. The results showed that both: the Standard and new protocols were comparable, and based on this, the new test was applied for further research. Results obtained adopting the new protocol with three antigens showed that two of them: Ag ES L1 T. spiralis (W) and Ag ES L1 T. spiralis are similar. The specific IgG antibodies for infective doses of 200 and 1000 larvae for these antigens were detectable 40 and 30 dpi respectively. In pigs infected with the highest dose of T. spiralis larvae IgG anti bodies were detectable from 20 dpi when Ag ES L1 T. spiralis was used. These results strongly indicate that in examined pigs, the specific IgG response to T. spiralis infection is dose dependant. Of 1474 examined pig sera only 0.99% gave a positive signal against ES L1 T. spiralis antigen. Of 1784 examined wild boars sera only 0.68 % gave positive results using the new ELISA protocol. ELISA is a useful method for detecting specific IgG antibodies in pigs experimentally infected with different doses of T. spiralis and naturally infected pigs. In pigs the specific IgG response is dose dependant. The Ag ES L1 T. spiralis increases the specifity of the method and reduces false positive results. Simultaneous use of both methods: digestion and ELISA for the diagnosis of Trichinella in naturally infected pigs and wild boars may increase the chances of eliminating meat infected with T. spiralis larvae.
Źródło:
Wiadomości Parazytologiczne; 2007, 53, 2; 149-151
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Efficacy of albendazole against Trichinella pseudospiralis and Trichinella spiralis in mice
Wpływ albendazolu na przebieg zakażenia Trichinella pseudospiralis i Trichinella spiralis u myszy
Autorzy:
Bany, J.
Lach, J.
Golińska, Z.
Powiązania:
https://bibliotekanauki.pl/articles/2152265.pdf
Data publikacji:
1992
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Trichinella pseudospiralis
zwierzeta doswiadczalne
Trichinella spiralis
parazytologia
albendazol
myszy
nicienie
pasozyty
inwazja pasozytnicza
Trichinellidae
Opis:
Albendazole (Smith Kline, Beecham) in a dose of 20 mg/kg/day was given to B6C3F₁ mice exposed to 300 larvae of T. pseudospiralis or T. spiralis. The drug was introduced on days 2, 3 and 4; or 4, 5 and 6; or 18, 19 and 20 after infection. It limited the intensity of intestinal and muscle phases of trichinellosis. Given at the stage of maturation of adult forms and production of new-born larvae, the drug caused almost total elimination of both species from the intestines of mice and a decrease in the numbers of muscle larvae, with a tendency towards a greater reduction of larvae and adults of T. pseudospiralis. The drug did not change the course of infection when given at the phase of infectiveness of larvae.
Źródło:
Wiadomości Parazytologiczne; 1992, 38, 3-4; 143-146
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
1D-immunoblot as a species-specific diagnostic tool in human trichinellosis: yes or no?
Autorzy:
Bien, J.
Gozdzik, K.
Moskwa, B.
Stefaniak, J.
Powiązania:
https://bibliotekanauki.pl/articles/6700.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
immunoblotting
diagnostic method
Trichinella spiralis
etiological agent
human infection
human trichinellosis
trichinellosis
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proby modyfikacji przechodzenia komorek nacieku zaplanego do torebek larw Trichinella spiralis
Autorzy:
Karmanska, K
Piekarska, J.
Powiązania:
https://bibliotekanauki.pl/articles/839866.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
przenikanie komorek
Trichinella spiralis
parazytologia
komorkowe nacieki zapalne
torebki larw
pasozyty
immunomodulatory
immunomodulacja
larwy
Opis:
The inflammatory infiltration cells penetrating through the larval capsule walls were observed. The observation were carried out in the masseter muscle of the mice infected with Trichinella spiralis larvae only (control) or infected and treated with PHA-P, TFX or dexamethason. The more numerous cells, than in the control, in the mice treated with PHA-P and TFX were seen. Opposite, the fever numerous cells inside the capsula walls in the mice that received dexamethason were found. The authors supposed that the phenomenon of inflammatory cells penetration through larval capsule play an important role in the defence mechanisms of the host.
Źródło:
Annals of Parasitology; 2001, 47, 1; 39-44
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dzialanie neuroimmunomodulacyjne wysokotoksycznego zwiazku fosforoorganicznego na rozwoj infekcji Trichinella spiralis
Autorzy:
Bany, J.
Zdanowska, D.
Powiązania:
https://bibliotekanauki.pl/articles/841463.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
neuroimmunomodulacja
zwiazki fosforoorganiczne
Trichinella spiralis
parazytologia
wlosien spiralny
ester izopropylowy kwasu metylofluorofosforowego
infekcja pasozytnicza
Źródło:
Annals of Parasitology; 2004, 50, 2; 135-141
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Działanie neuroimmunomodulacyjne wysokotoksycznego związku fosforoorganicznego na rozwój infekcji Trichinella spiralis
Neuroimmunomodulatory effect of highly toxic organophosphorus compound on the development of Trichinella spiralis infection.
Autorzy:
Bany, J.
Zdanowska, D.
Powiązania:
https://bibliotekanauki.pl/articles/2146443.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
neuroimmunomodulacja
zwiazki fosforoorganiczne
Trichinella spiralis
parazytologia
wlosien spiralny
ester izopropylowy kwasu metylofluorofosforowego
infekcja pasozytnicza
Opis:
The highly toxic organophosphorus compound (isopropylmethylphosphonofluoridate — IMPF) belonging to the group of the so called toxic of warfare agents can impair immune effector mechanisms determining the development of parasitic infection. Preliminary studies have demonstrated that IMPF delays elimination of adults Trichinella spiralis forms from the intestine of C57B1/6 mice and B6C3F1 hybrids. Besides, the studied compound modifies the proliferative activity of T-cells in the above mentioned mice and reduces the production of anti-SRBC antibodies in C57B1/6 strain. The mechanisms are discussed of the organophosphorus compound effect on neuroimmunological processes connected with antiparasitic immunity, paying attention also to the function of these processes by the parasitic infection itself, in host-parasite relationship. The probability is also considered of increased sensitivity to Leishmania infection in humans poisoned with IMPF and/or other organophosphorus compounds.
Źródło:
Wiadomości Parazytologiczne; 2004, 50, 2; 135-141
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative ultrastructural studies of the alterations to mouse hepatic parenchyma during Trichinella spiralis or Toxocara canis infection
Autorzy:
Dabrowska, J.
Dybicz, M.
Powiązania:
https://bibliotekanauki.pl/articles/6278.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
ultrastructure
alteration
mouse
mice
hepatic parenchyma
parenchyma
Trichinella spiralis
Toxocara canis
infection
pathological change
liver
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Próby modyfikacji przechodzenia komórek nacieku zapalnego do torebek larw Trichinella spiralis
Autorzy:
Karmańska, K.
Piekarska, J.
Powiązania:
https://bibliotekanauki.pl/articles/2147833.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
przenikanie komorek
Trichinella spiralis
parazytologia
komorkowe nacieki zapalne
torebki larw
pasozyty
immunomodulatory
immunomodulacja
larwy
Opis:
The inflammatory infiltration cells penetrating through the larval capsule walls were observed. The observation were carried out in the masseter muscle of the mice infected with Trichinella spiralis larvae only (control) or infected and treated with PHA-P, TFX or dexamethason. The more numerous cells, than in the control, in the mice treated with PHA-P and TFX were seen. Opposite, the fever numerous cells inside the capsula walls in the mice that received dexamethason were found. The authors supposed that the phenomenon of inflammatory cells penetration through larval capsule play an important role in the defence mechanisms of the host.
Źródło:
Wiadomości Parazytologiczne; 2001, 47, 1; 39-44
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of aqueous extract from Scutellaria baicalensis Georgi roots on CD4+ and CD8+ T cell responses during experimental infection with Trichinella spiralis in mice
Autorzy:
Piekarska, J.
Szczypka, M.
Gorczykowski, M.
Króliczewska, B.
Miśta, D.
Oszmiański, J.
Powiązania:
https://bibliotekanauki.pl/articles/2087259.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Scutellaria baicalensis
Trichinella spiralis
immunomodulation
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 4; 501-510
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The phenomenon of apoptosis in the course of experimental trichinellosis in mice
Autorzy:
Karmanska, K
Houszka, M.
Piekarska, J.
Powiązania:
https://bibliotekanauki.pl/articles/837779.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
mouse
mice
trichinellosis
larva
apoptosis
Trichinella spiralis
Opis:
Mice infected with 200 Trichinella spiralis larvae were killed at 3-69 days post infection (dpi) and the jejunum and masseter muscles were sectioned in a cryostat and examined in the Tuneli method with "In situ Cell Detected Kid POD" of Boehringer-Mannheim. Data concering the exact localization and dynamie of apoptic cells in both organs are presented. The authors conclude that apoptosis plays a important role in the pathogenesis of trichinellosis.
Źródło:
Annals of Parasitology; 2000, 46, 1
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 38

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