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    Tytuł:
    Stan badan nad wykorzystaniem czynnikow biologicznych do ograniczania populacji owadow zerujacych na korzeniach
    Autorzy:
    Malinowski, H
    Powiązania:
    https://bibliotekanauki.pl/articles/53310.pdf
    Data publikacji:
    1998
    Wydawca:
    Instytut Badawczy Leśnictwa
    Tematy:
    zwalczanie szkodnikow
    bakterie entomopatogenne
    nicienie owadobojcze
    grzyby entomopatogenne
    biologiczne metody ochrony roslin
    szkodniki korzeni
    ochrona lasu
    pedraki
    lesnictwo
    Źródło:
    Prace Instytutu Badawczego Leśnictwa. Seria A; 1998, 856-862; 103-128
    0369-9870
    Pojawia się w:
    Prace Instytutu Badawczego Leśnictwa. Seria A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Aktywność grzybów entomopatogennych wobec leśnych foliofagów i kambiofagów
    Activity of entomopathogenic fungi against forest insects feeding on leaves, bark and cambium
    Autorzy:
    Malinowski, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/973785.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    foliofagi
    grzyby entomopatogenne
    kambiofagi
    ochrona lasu
    zwalczanie szkodnikow
    leśnictwo
    biologiczne metody ochrony roślin
    biological control
    forest harmful insects
    entomopathogenic fungi
    Opis:
    The paper presents results of various experiments on the use of entomopathogenic fungi (e.g. Beauveria bassiana, B. brongniartii, Metarhizium anisopliae, Lecanicllium spp.) to control leaf feeding insects (Lymantria spp., Dendrolimus pini, Cephalcia abietis, Diprionidae) and insects feeding on bark and cambium (Hylobius spp., Scolytinae)
    Źródło:
    Sylwan; 2011, 155, 03; 188-194
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Charakterystyka enzymow proteo-, chityno- i lipolitycznych pasozytniczego grzyba Conidiobolus coronatus
    Characterization of proteo-, chitino- and lipolytic enzymes of parasitic fungus Conidiobolus coronatus
    Autorzy:
    Wloka, E
    Powiązania:
    https://bibliotekanauki.pl/articles/836974.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    grzyby pasozytnicze
    grzyby entomopatogenne
    Conidiobolus coronatus
    Entomophthorales
    enzymy proteolityczne
    elastaza
    N-acetyloglukozaminidaza
    enzymy chitynolityczne
    enzymy lipolityczne
    lipaza
    aktywnosc enzymatyczna
    owady
    barciak wiekszy
    Galleria mellonella
    zwalczanie szkodnikow
    metody biologiczne
    grzyby owadobojcze
    Źródło:
    Annals of Parasitology; 2010, 56, 1; 83-85
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Charakterystyka enzymów proteo-, chityno- i lipolitycznych pasożytniczego grzyba Conidiobolus coronatus
    Characterization of proteo-, chitino- and lipolytic enzymes of parasitic fungus Conidiobolus coronatus
    Autorzy:
    Włóka, E
    Powiązania:
    https://bibliotekanauki.pl/articles/2143413.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    grzyby pasozytnicze
    grzyby entomopatogenne
    Conidiobolus coronatus
    Entomophthorales
    enzymy proteolityczne
    elastaza
    N-acetyloglukozaminidaza
    enzymy chitynolityczne
    enzymy lipolityczne
    lipaza
    aktywnosc enzymatyczna
    owady
    barciak wiekszy
    Galleria mellonella
    zwalczanie szkodnikow
    metody biologiczne
    grzyby owadobojcze
    Opis:
    The largest problem in limitation of insect pest population is increasing resistance of them to chemical pesticides. Alternative are entomopathogens, which regulate frequency of insect pests. Among them decisive role play entomopathogenic fungi, which possess the ability to active penetration through cuticle by mechanical pressure of invasive hypha and production of proteo-, chitino- (egzo- and endochitinases) as well as lipolytic enzymes, which provide nutrients for subsequent development of fungus. Entomopathogenic soil fungus Conidiobolus coronatus (Entomophtorales) is saprophyte fungus, which demonstrates a high efficiency in the paralysis of varied insects. Although leading investigations over mechanism of insect paralysis, we still do not know, what role fungal enzymes play in insect cuticle penetration. The main aim of research was establishment of optimal conditions for elastase, N-acetylglucosaminidase (NAGase), chitobiosidase as well as lipase. Optimal reaction parameters were determined: volume of reaction mixture, volume of homogenate, working pH and the substrate concentration. Having on aim a possible use of C. coronatus in pest control, two ranges of temperatures were chosen: 20°C – optimal temperature for the fungus growing and 30°C – optimal temperature for the cultivation of the great wax moth larvae, Galleria mellonella, on which examinations were performed. Also kinetic constants Km and Vmax were determined. Activity of elastase and N-acetylglucosaminidase of C. coronatus was measured spectrophotometrically at 410 nm (towards N-Succinyl-Ala-Ala-Pro-Leu-p-Nitroanilide) and 405 nm (towards 4-Nitrophenyl-N-acetyl-b-D-glucosaminide), respectively. The following optimal conditions of elastase activity were established: the volume of reaction mixture 0.5 ml, volume of homogenate 1 ml, temperature 30°C, pH 8, substrate concentration 40 mM. Optimal conditions of NAGase assay: the volume of reaction mixture 0.5 ml, dose of homogenate 12.5 ml, temperature 30°C, pH neutral and 6 mM substrate concentration. The activities of chitobiosidase and lipase were measured spectrofluorometrically (Ex=360 nm, Em=450 nm) towards 4-Methylumbelliferyl b-D-N-N’-diacetylchitobioside and 4-Methylumbelliferyl oleate, respectively. Chitobiosidase showed the highest activity in dose of 30 ml in 1 ml volume of reaction mixture, at the temperature of 30°C, pH 7 and substrate concentration equal to 2 mM. Lipase showed the highest catalytic activity in 1 ml volume of reaction mixture, in 30°C but 50 ml of homogenate, pH 10 and 10 mM substrate concentration were needed. Higher activity investigated enzymes in 30°C than 20°C indicated that they can take part in pathogenesis. It was suggested that as first in perforation of coats of insects body elastase and lipase take part. Indicated of it, large thermoresistance of both enzymes (only 10.5% decrease of elastase activity at 20°C and 9.4% decrease of lipase activity in comparison with maximal activity at 30°C), alkalophilicity of both proteins (elastase shows the alkaline optimal pH equal to 8 at pH 9 preserves 97% activity, and at pH 10 94% activity, respectively while lipase prefers the pH 10 and at pH 8 and pH 9 enzyme keeps 57 and 60% activity, respectively) as well as lack of repression by suitable substrates. Sigmoid character of curve concerning pH influence on the activity of both enzymes, also indicates similarity between elastase and lipase. On minor part of NAGase and chitobiosidase of fungus C. coronatus in perforation of coats of host body showed high sensibility of both enzymes on hydrogen ions concentration: both enzymes prefer neutral pH, in pH 6 and 8 lose over 35% activity but subjection to substrate repression and 3–4-fold growth of activity followed only in 30°C. In the course of work it was found, that rich medium (LB) stimulates growth of mycelium and production of fungal lipases. So far nobody managed to isolate chitinolytic or lipolytic enzymes from C. coronatus homogenate. The majority of fungal enzymes were isolated from post incubation filtrates. In the literature of the subject lack of data about C. coronatus NAGase, therefore in examinations also the trial of isolation NAGase from C. coronatus homogenate was undertaken. Activity of NAGase showed only first fraction, which did not separate with none of used columns. Disappointing results of purification on cation exchanger CM, weak anion exchanger DEAE, and strong anion exchanger Q were obtained as well as after fractionation tests with the use of Microcon microcolumns. In aim of NAGase molecular mass estimation, two zymograms were made with Triton X-100 and casein and with the use of fluorescent substrate 4-Methylumbelliferyl N-acetyl-b-D-glucosaminide. Molecular mass of NAGase from C. coronatus was established on ca. 60 kDa. This is the first report describing molecular weight of NAGase from C. coronatus. Examined NAGase has different properties than known NAGases from other entomopathogenic fungi. Although its molecular weight is equal to the Metarhizium anisopliae NAGase, optimal pH for both NAGases are different: neutral in the case of C. coronatus NAGase versus acidic in the case of M. anisopliae NAGase. Knowledge of molecular mass of the C. coronatus NAGase should allow to find a new method of this enzyme isolation from C. coronatus homogenate. Thanks to developed methods of assaying activities of elastase, NAGase, chitobiosidase and lipase, real becomes the understanding of mechanism of insects paralysis through C. coronatus fungus.
    Źródło:
    Wiadomości Parazytologiczne; 2010, 56, 1; 83-85
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Próba zastosowania grzyba Beauveria bassiana (Bals.) Vuill. w ochronie lasu przed kornikiem drukarzem Ips typographus (L.) w warunkach terenowych
    An attempt to use the fungus Beauveria bassiana (Bals.) Vuill. in forest protection against the bark beetle Ips typographus (L.) in the field
    Autorzy:
    Grodzki, W.
    Kosibowicz, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/1312103.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Badawczy Leśnictwa
    Tematy:
    lesnictwo
    ochrona lasu
    lasy gorskie
    drzewostany swierkowe
    szkodniki roslin
    kornik drukarz
    Ips typographus
    zwalczanie szkodnikow
    grzyby entomopatogenne
    Beauveria bassiana
    biopreparaty
    badania terenowe
    bark beetles
    Picea abies
    biological control
    montane forests
    field trials
    Opis:
    In 2011–2013, trials on the use of the entomopathogenic fungus Beauveria bassiana against bark beetle (Ips typographus) populations were carried out under open field conditions in Norway spruce stands suffering from an outbreak in the Beskid Żywiecki Mts. in Poland. Modified pheromone traps were deployed to capture and thereafter release fungus-infected bark beetles to the forest environment. Infested spruce trees felled next to the traps remained unaffected by the transmission of the fungus to insect populations. Direct spraying or dusting of lying trap logs and suspended caged rearing bolts did not have any effect on spruce infestation by I. typographus, its reproduction success and development or natural enemies inside the bark. A very small effect on mortality rates of target as well as non-target insects overwintering in the dusted litter was observed. Treated stands, unlike control stands, were indirectly affected by the treatment, evidenced by the reduction of tree mortality due to bark beetle infestation. At present, no recommendations concerning the potential use of the fungus in forest protection can be given. However such an environmentally friendly approach represents a promising future prospect.
    Źródło:
    Leśne Prace Badawcze; 2015, 76, 1; 5-17
    1732-9442
    2082-8926
    Pojawia się w:
    Leśne Prace Badawcze
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Możliwości ochrony lasu przed owadami uszkadzającymi systemy korzeniowe metodą biologiczną z wykorzystaniem grzybów entomopatogennych. I. Mechanizm zakażania owadów grzybami entomopatogennymi i czynniki wpływające na infekcję
    Possibility of forest protection against insects damaging root systems with the use of biological method based on entomopathogenic fungi. I. Mechanism of insects infection with entomopathogenic fungi and factors...
    Autorzy:
    Malinowski, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/1009635.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    zwalczanie szkodnikow
    grzyby entomopatogenne
    biologiczne metody ochrony roslin
    Metarhizium anisopliae
    owady
    szkodniki korzeni
    ochrona lasu
    lesnictwo
    Verticillium lecanii
    Beauveria brongniartii
    insects infection
    entomopathogenic fungi
    beauveria brongniartii
    metarhizium anisopliae
    verticillium lecanii
    insects control
    Opis:
    The paper comprises basic knowledge on the mechanism of insects infection with entomopathogenic fungi (Beauveria brongniartii, Metarhizium anisopliae, Verticillium lecanii) and factors influencing this process. It depends on pathogen properties, host susceptibility, and biotic and abiotic elements of environment. Such information is useful to understand the slower effectiveness of mycoinsecticides than chemical insecticides.
    Źródło:
    Sylwan; 2009, 153, 12; 795-804
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Możliwości ochrony lasu przed owadami uszkadzającymi systemy korzeniowe metodą biologiczną z wykorzystaniem grzybów entomopatogennych. II. Skuteczność bioinsektycydów grzybowych w ograniczaniu liczebności pędraków chrabąszczy (Melolontha spp.) i innych szkodników korzeni
    Possibility of forest protection against insects damaging root systems with the use of biological method based on entomopathogenic fungi. II. Effectiveness of fungal bioinsecticides against Melolontha spp. white grubs and other pests of roots
    Autorzy:
    Malinowski, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/1009527.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    ochrona lasu
    szkodniki korzeni
    owady
    chrabaszcz
    Melolontha
    pedraki
    zwalczanie szkodnikow
    biologiczne metody ochrony roslin
    grzyby entomopatogenne
    Beauveria brongniartii
    Metarhizium anisopilae
    Verticillium lecanii
    biopreparaty grzybowe
    skutecznosc
    white grubs control
    fungal biopreparations
    beauveria brongniartii
    metarhizium anisopliae
    and verticillium lecanii
    Opis:
    The paper presents results of various studies on the use of entomopathogenic fungi (Beauveria brongniartii, Metarhizium anisopliae, and Verticillium lecanii) to control pests of roots in forest nurseries and plantations, especially Melolontha spp. white grubs.
    Źródło:
    Sylwan; 2010, 154, 01; 15-23
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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