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Wyszukujesz frazę "tissue culture" wg kryterium: Temat


Tytuł:
Methods of eliminating contaminations in tissue cultures on the example of selected ornamental plant species
Autorzy:
Parzymies, M.
Dabski, M.
Powiązania:
https://bibliotekanauki.pl/articles/951204.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
tissue culture
contamination
ornamental plant
Clematis
biological contamination
micropropagation
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Economics analysis of Seed Yam production in Aeroponic system
Autorzy:
Acheampong, Patricia Pinamang
Quain, Marian Dorcas
Appiah-Kubi, David
Osei-Adu, Jonas
Ennin, Stella Ama
Osei, Kingsley
Powiązania:
https://bibliotekanauki.pl/articles/1892087.pdf
Data publikacji:
2020-07-08
Wydawca:
Uniwersytet Przyrodniczy w Poznaniu. Wydawnictwo Uczelniane
Tematy:
Benefit-cost ratio
net revenue
payback period
private sector
tissue culture
Opis:
The supply of seed yams for intensive yam production is hindered by many constraints, including diseases and pest infestations as well as the unavailability of quality planting material. The combination of tissue culture and aeroponics system is perceived to be the way towards clean and adequate supply of seed yam for enhanced yam production. However, the system is considered as expensive for any individual to implement. In order to encourage private sector to participate in this venture, the economic analysis determining the feasibility and viability of using aeroponics in seed yam production was performed. Using data from established tissue culture and aeroponics system in Ghana, the fixed cost and variable cost parameters as well as production costs were obtained. Results revealed that total cost of building aeroponics structure amounted to GH₵ 94,178.00 (USD 17,938.70). Annual cost of aeroponics structure was GH₵ 9,417.82 (USD 1,793.87). Annual total cost of production totalled GH₵ 204,391.75 (USD 38,931.61). Annual net revenue was GH₵ 75,888.00 (USD 14,454.86). Payback period was 15 months and benefit cost ratio was 1.4. Aeroponics system for seed yam production is therefore profitable since short period would be needed to recoup investment. For food security and creation of workplaces, government could partner with the private sector in the establishment of aeroponics systems to increase yam production and export.
Źródło:
Journal of Agribusiness and Rural Development; 2020, 56, 2; 125-132
1899-5241
Pojawia się w:
Journal of Agribusiness and Rural Development
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nuclear morphology, polyploidy, and chromatin elimination in tissue culture of Allium fistulosum L.
Autorzy:
Joachimiak, A
Ilnicki, T.
Powiązania:
https://bibliotekanauki.pl/articles/59216.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
chromatin
heterochromatin
tissue culture
polyploidy
Alliaceae
morphology
Monocotyledoneae
micronucleus
Allium fistulosum
Opis:
The morphology of cell nuclei in callus obtained from root-tip meristems of Allium fistulosum L. (Monocotyledoneae, Alliaceae) was analysed. The most interesting phenomena observed in long-term callus culture were the different mechanisms of cell polyploidization, enlargement of telomeric segments of heterochromatin, and extensive chromatin elimination, associated with instability of nuclei size and DNA content. Protruding heterochromatin "spikes" were observed on the surface of some di- and polyploid nuclei. The presence of these spikes was connected with the formation of small heterochromatic micronuclei frequently found in the cytoplasm. It is suggested that these micronuclei are produced by direct elimination of heterochromatin from the interphase nuclei. Polyploid cells accumulated with each successive cell collection. The ploidy level attained by highly polyploid cells was 15C-220C. The shape of the nuclei and heterochromatin distribution suggest that polyploid nuclei in A. fistulosum tissue culture are produced by endoreduplication and by restitution cycles.
Źródło:
Acta Societatis Botanicorum Poloniae; 2003, 72, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro propagation of Nepalese orchids: a review
Autorzy:
da Silva, J.A.T.
Acharya, K.P.
Powiązania:
https://bibliotekanauki.pl/articles/1932.pdf
Data publikacji:
2014
Wydawca:
Instytut Ogrodnictwa
Tematy:
in vitro propagation
Nepal
Orchidaceae
orchid
seed germination
tissue culture
germplasm
Źródło:
Journal of Horticultural Research; 2014, 22, 2
2300-5009
Pojawia się w:
Journal of Horticultural Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cloning, expression and genetic transformation of sucrose phosphate synthase (SPS) gene in Saccharum spontaneum L.
Autorzy:
Zhang, Yun-Wei
Zhou, Yun-Zhuan
Lu, Hai-Bo
Zheng, Deng-Yu
Huang, Yan-Hua
Powiązania:
https://bibliotekanauki.pl/articles/952058.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
saccharum spontaneum l.
sps gene
cloning
expression
tissue culture
genetic transformation
Opis:
Sucrose phosphate synthase (SPS) is a key enzyme catalyzing sucrose metabolism in plants. In this study, we isolated the SPS cDNA from Saccharum spontaneum and designated as SsSPS (GenBank accession no. MF398541). The full-length of SsSPS cDNA was 4153-bp with an opening reading frame (ORF) of 3132 nucleotides, which encoded a 1043-amino acid protein. The nucleotide sequences alignment showed that it had 98%, 97% and 87% homology with S. officinarum, Setaria italica and Lolium perenne, respectively. Moreover, the SsSPS was detected to express in leaf and stem tissues of S. spontaneum and exhibited a predominant expression in the stem tissue. However, there was no significant difference in the expression level of SsSPS between young leaves and mature ones. Additionally, we generated transgenic S. spontaneum using Agrobacterium-mediated transformation. Our data will provide a valuable foundation for further study of the potential role of SPS in plants.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2017, 59, 2
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro responses of various explants of Fagus sylvatica
Autorzy:
Hazubska-Przybyl, T.
Chmielarz, P.
Bojarczuk, K.
Powiązania:
https://bibliotekanauki.pl/articles/41768.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
European beech
plant response
explant
Fagus sylvatica
micropropagation
culture condition
tissue culture
in vitro culture
Opis:
There are limited published data on in vitro reproduction of Fagus sylvatica L. (European beech). This study was aimed to determine the efficiency of induction of somatic embryogenesis or organogenesis of beech from different types of explants in various culture conditions. Explants derived from immature, fresh seeds (collected in 2011 and 2013) and from mature seeds, stored at –10ºC and some stratified at 3ºC, were placed on induction media with various combinations of plant growth regulators: zeatin, 2,4-dichlorophenoxyacetic acid (2,4-D) and/or benzyladenine (BA). Initial cultures were kept in darkness or weak light (white fluorescent or blue-red LED). Limited success has been achieved in initiation of somatic embryogenesis. We obtained friable, yellow-white callus with characteristic PEM-like structures (cPEM-ls, from embryonic axes or fragments of immature embryos with embryonic axes), which may be an early developmental stage of embryogenic callus of Fagus sylvatica. This type of callus regenerated from explants incubated in darkness, mainly on WPM medium with addition of 6.8 μM zeatin or WPM and MSG media with 9.1 μM 2,4-D and 2.2 μM BA. The highest frequency of regeneration of callus with cPEM-ls was 5%. Instead, we succeeded to induce organogenesis from both immature and mature zygotic embryos and from embryonic axes. The best results were obtained for mature zygotic embryos incubated on ½WPM medium (half-strength Woody Plant Medium) with 9.1 μM 2,4-D and 2.2 μM BA. Adventitious buds were regenerated on up to 15% of the explants. The induced buds developed into shoots, enabling us to establish tissue cultures of beech. Induction of organogenesis from the tested explants was more efficient than induction of somatic embryogenesis.
Źródło:
Dendrobiology; 2015, 73
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro cultures of animal and human cells
Autorzy:
Slomski, R.
Nowak, A.
Hryhorowicz, M.
Powiązania:
https://bibliotekanauki.pl/articles/951215.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
in vitro culture
tissue culture
animal cell
human cell
homeostasis
fluorescence in situ hybridization
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phenolics in undifferentiated tissue cultures of Cichorieae
Autorzy:
Malarz, J.
Stojakowska, A.
Powiązania:
https://bibliotekanauki.pl/articles/79813.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
phenolics
tissue culture
Lactuca virosa
Cichorium intybus
Lactuca aculeata
sesquiterpene lactone
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A mathematical model of HIV-1 infection including the saturation effect of healthy cell proliferation
Autorzy:
Kouche, M.
Ainseba, B.
Powiązania:
https://bibliotekanauki.pl/articles/907704.pdf
Data publikacji:
2010
Wydawca:
Uniwersytet Zielonogórski. Oficyna Wydawnicza
Tematy:
zakażenie HIV
stabilność
hodowla tkanek
HIV
periodic oscillations
persistence
stability
tissue culture
Opis:
In this paper we derive a model describing the dynamics of HIV-1 infection in tissue culture where the infection spreads directly from infected cells to healthy cells trough cell-to-cell contact. We assume that the infection rate between healthy and infected cells is a saturating function of cell concentration. Our analysis shows that if the basic reproduction number does not exceed unity then infected cells are cleared and the disease dies out. Otherwise, the infection is persistent with the existence of an infected equilibrium. Numerical simulations indicate that, depending on the fraction of cells surviving the incubation period, the solutions approach either an infected steady state or a periodic orbit.
Źródło:
International Journal of Applied Mathematics and Computer Science; 2010, 20, 3; 601-612
1641-876X
2083-8492
Pojawia się w:
International Journal of Applied Mathematics and Computer Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of stress conditions on acclimation of magnolia Magnolia x soulangiana Soul.-Bod.
Wpływ warunków stresu na aklimatyzację magnolii Magnolia x soulangiana Soul.-Bod.
Autorzy:
Kamenicka, A.
Lanakova, M.
Cerna, K.
Powiązania:
https://bibliotekanauki.pl/articles/808264.pdf
Data publikacji:
2002
Wydawca:
Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Tematy:
stress condition
acclimation
magnolia
Magnolia x soulangiana
tissue culture
leaf cell
donor plant
Opis:
Under a scanning microscope, leaves from tissue cultures (after 8 weeks in vitro culturing), from 1 year old regenerants and from the 100 years old donor tree were studied. More abnormalities were observed in the tissue culture compared to regenerant and donor. During tissue culture transfer rapid, leaf dehydration occurs due to defective regulation mechanisms making stomata of tissue culture stay open. Epidermis as well as stomata cells were observed already on the leaves in in vitro conditions, but they become physiologically functioning only after transfer into conditions of natural environment.
Przy zastosowaniu mikroskopu scaningowego porównywano cechy budowy komórek liści pochodzących z 100 letniej rośliny matecznej, 8 tygodniowych kultur tkankowych oraz 1 rocznych regeneratów. W regenerantach z kultur tkankowych obserwowano więcej nienormalności w budowie komórek epidermy, komórek szparkowych i komórek przyszparkowych w porównaniu z komórkami pochodzącymi z rośliny matecznej. Podczas przenoszenia tkanek w kulturze in vitro obserwowano odwodnienie tkanek liści a mimo to komórki szparkowe pozostawały otwarte. Komórki epidermy oraz komórki szparkowe były obecne na liściach hodowli in vitro jednak stawały się one fizjologicznie funkcjonalne tylko wtedy gdy zostały przeniesione do warunków naturalnych.
Źródło:
Zeszyty Problemowe Postępów Nauk Rolniczych; 2002, 481, 1
0084-5477
Pojawia się w:
Zeszyty Problemowe Postępów Nauk Rolniczych
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rozmnazanie ziemniaka 'in vitro' w Instytucie Ziemniaka w Boninie
Autorzy:
Zaklukiewicz, K
Sekrecka, D.
Powiązania:
https://bibliotekanauki.pl/articles/835057.pdf
Data publikacji:
1992
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
ziemniaki
kultury tkankowe
rozmnazanie roslin
hodowla in vitro
potato
tissue culture
plant propagation
in vitro culture
Źródło:
Ziemniak Polski; 1992, 3; 41-44
1425-4263
Pojawia się w:
Ziemniak Polski
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Embryogenic callus induction and differentiation in silver fir (Abies alba Mill.) tissue cultures
Autorzy:
Nawrot-Chorabik, K
Powiązania:
https://bibliotekanauki.pl/articles/41643.pdf
Data publikacji:
2008
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
silver fir
Abies alba
tissue culture
embryogenic suspensor mass
somatic embryogenesis
callus induction
differentiation
Opis:
The research was conducted on explants of silver fir (Abies alba Mill.) deriving from several forest districts in southern Poland. The study encompassed the influence of the origin of plant material, type of explants, kind of substances used for explants sterilization, PPM and the type of medium on the ability to form embryogenic callus and to develop somatic embryos in silver fir explants. From the plant material collected in three sites, 57 clones were obtained from mature zygotic embryos; this produced an embryogenesis frequency of 6%. Embryogenic callus was obtained with a diameter of 65–70 mm depending on the material origin. The best medium for development of callus inducted on embryos isolated from mature silver fir seeds was the SH medium. Somatic embryos were formed in a globular stadium (24 pieces) on this medium. The 10% solution of NaOCl (used for 15 minutes) turned out to be the most effective substance for seed sterilization.
Źródło:
Dendrobiology; 2008, 59; 31-40
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants
Autorzy:
Li, J.
Kang, Y.
Qiang, S.
Peng, G.
Powiązania:
https://bibliotekanauki.pl/articles/56457.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
propagation
goldenrod
Solidago canadensis
leaf
nodal explant
callus formation
micropropagation
adventitious shoot
tissue culture
Opis:
Goldenrod (Solidago canadensis L.) is an invasive plant species in many countries except North America but a cut-flower species worldwide. There is a need to generate and propagate goldenrod clones efficiently for research and commercial purposes. A callus induction and plantlet regeneration system was developed by studying the influence of explant type and different concentrations of plant growth regulators. The highest callus production from leaf segments was obtained on Murashige and Skoog's medium (MS medium) supplemented with 1.0 mg/L naphthalene acetic acid (NAA) and 1.0 mg/L 6-benzylaminopurine (BA). Adventitious shoots could be regenerated directly from leaf explants without an intermediate callus phase with the highest shoot induction percentage of 87.2%. The largest number of adventitious shoots per leaf explant (3.2) was obtained on MS medium supplemented with 0.4 mg/L NAA and 2.0 mg/L BA. MS medium supplemented with 0.1 mg/L NAA and 1.0 mg/L BA was the best medium for axillary shoot regeneration from nodal segments. The highest root number and longest roots occurred on half-strength MS without the addition of any growth regulator. Rooted plantlets were then transferred to a soil-based growth medium, placed in a greenhouse, and acclimatized with 100% success. All surviving plants grew normally without showing any morphological variation when compared to those grow from seed. This regeneration protocol may be used to produce certain biotypes of goldenrod suitable for genetic transformation, rapid propagation of goldenrod for commercial purposes or for screening fungi and toxins as potential biocontrol agents against this weed.
Źródło:
Acta Societatis Botanicorum Poloniae; 2012, 81, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative study on tissue culture induced variation identified with metAFLP and RP – HPLC in barley and triticale regenerants
Autorzy:
Machczynska, J.
Orlowska, R.
Ogorek, K.A.
Bednarek, P.T.
Powiązania:
https://bibliotekanauki.pl/articles/951298.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
tissue culture
in vitro culture
plant regeneration
genetic change
DNA methylation
histone modification
high performance liquid chromatography
barley
triticale
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Neural modeling of plant tissue cultures: a review
Autorzy:
Zielinska, S.
Kepczynska, E.
Powiązania:
https://bibliotekanauki.pl/articles/81293.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
artificial neural network
biomass
plant tissue
neural model
tissue culture
in vitro condition
micropropagation
radial neural network
neural network
somatic embryo
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł

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