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Wyświetlanie 1-15 z 18
Tytuł:
Methods of eliminating contaminations in tissue cultures on the example of selected ornamental plant species
Autorzy:
Parzymies, M.
Dabski, M.
Powiązania:
https://bibliotekanauki.pl/articles/951204.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
tissue culture
contamination
ornamental plant
Clematis
biological contamination
micropropagation
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro responses of various explants of Fagus sylvatica
Autorzy:
Hazubska-Przybyl, T.
Chmielarz, P.
Bojarczuk, K.
Powiązania:
https://bibliotekanauki.pl/articles/41768.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
European beech
plant response
explant
Fagus sylvatica
micropropagation
culture condition
tissue culture
in vitro culture
Opis:
There are limited published data on in vitro reproduction of Fagus sylvatica L. (European beech). This study was aimed to determine the efficiency of induction of somatic embryogenesis or organogenesis of beech from different types of explants in various culture conditions. Explants derived from immature, fresh seeds (collected in 2011 and 2013) and from mature seeds, stored at –10ºC and some stratified at 3ºC, were placed on induction media with various combinations of plant growth regulators: zeatin, 2,4-dichlorophenoxyacetic acid (2,4-D) and/or benzyladenine (BA). Initial cultures were kept in darkness or weak light (white fluorescent or blue-red LED). Limited success has been achieved in initiation of somatic embryogenesis. We obtained friable, yellow-white callus with characteristic PEM-like structures (cPEM-ls, from embryonic axes or fragments of immature embryos with embryonic axes), which may be an early developmental stage of embryogenic callus of Fagus sylvatica. This type of callus regenerated from explants incubated in darkness, mainly on WPM medium with addition of 6.8 μM zeatin or WPM and MSG media with 9.1 μM 2,4-D and 2.2 μM BA. The highest frequency of regeneration of callus with cPEM-ls was 5%. Instead, we succeeded to induce organogenesis from both immature and mature zygotic embryos and from embryonic axes. The best results were obtained for mature zygotic embryos incubated on ½WPM medium (half-strength Woody Plant Medium) with 9.1 μM 2,4-D and 2.2 μM BA. Adventitious buds were regenerated on up to 15% of the explants. The induced buds developed into shoots, enabling us to establish tissue cultures of beech. Induction of organogenesis from the tested explants was more efficient than induction of somatic embryogenesis.
Źródło:
Dendrobiology; 2015, 73
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rozmnazanie ziemniaka 'in vitro' w Instytucie Ziemniaka w Boninie
Autorzy:
Zaklukiewicz, K
Sekrecka, D.
Powiązania:
https://bibliotekanauki.pl/articles/835057.pdf
Data publikacji:
1992
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
ziemniaki
kultury tkankowe
rozmnazanie roslin
hodowla in vitro
potato
tissue culture
plant propagation
in vitro culture
Źródło:
Ziemniak Polski; 1992, 3; 41-44
1425-4263
Pojawia się w:
Ziemniak Polski
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of stress conditions on acclimation of magnolia Magnolia x soulangiana Soul.-Bod.
Wpływ warunków stresu na aklimatyzację magnolii Magnolia x soulangiana Soul.-Bod.
Autorzy:
Kamenicka, A.
Lanakova, M.
Cerna, K.
Powiązania:
https://bibliotekanauki.pl/articles/808264.pdf
Data publikacji:
2002
Wydawca:
Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Tematy:
stress condition
acclimation
magnolia
Magnolia x soulangiana
tissue culture
leaf cell
donor plant
Opis:
Under a scanning microscope, leaves from tissue cultures (after 8 weeks in vitro culturing), from 1 year old regenerants and from the 100 years old donor tree were studied. More abnormalities were observed in the tissue culture compared to regenerant and donor. During tissue culture transfer rapid, leaf dehydration occurs due to defective regulation mechanisms making stomata of tissue culture stay open. Epidermis as well as stomata cells were observed already on the leaves in in vitro conditions, but they become physiologically functioning only after transfer into conditions of natural environment.
Przy zastosowaniu mikroskopu scaningowego porównywano cechy budowy komórek liści pochodzących z 100 letniej rośliny matecznej, 8 tygodniowych kultur tkankowych oraz 1 rocznych regeneratów. W regenerantach z kultur tkankowych obserwowano więcej nienormalności w budowie komórek epidermy, komórek szparkowych i komórek przyszparkowych w porównaniu z komórkami pochodzącymi z rośliny matecznej. Podczas przenoszenia tkanek w kulturze in vitro obserwowano odwodnienie tkanek liści a mimo to komórki szparkowe pozostawały otwarte. Komórki epidermy oraz komórki szparkowe były obecne na liściach hodowli in vitro jednak stawały się one fizjologicznie funkcjonalne tylko wtedy gdy zostały przeniesione do warunków naturalnych.
Źródło:
Zeszyty Problemowe Postępów Nauk Rolniczych; 2002, 481, 1
0084-5477
Pojawia się w:
Zeszyty Problemowe Postępów Nauk Rolniczych
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of explants type and orientation on growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro
Autorzy:
Kozak, D.
Parzymies, M.
Świstowska, A.
Marcinek, B.
Ismael, B.S.
Powiązania:
https://bibliotekanauki.pl/articles/12663898.pdf
Data publikacji:
2019
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
plant cultivation
Brazilian jasmine
Mandevilla sanderi
ornamental plant
flowering plant
pot plant
commercial plant
plant growth
plant development
plant regeneration
explant type
multiplication
decapitation
defoliation
in vitro culture
tissue culture
cultivation experiment
Opis:
Mandevilla sanderi is an important commercial ornamental pot plant. Traditional vegetative propagation is limited due to the low rate, therefore there is a need to develop an alternative, more efficient method. There is an interest in development of micropropagation technology for the species, as it allows to obtain a lot of offsprings in a relatively short time. The aim of the present work was to estimate an influence of explants type and position on regeneration of Mandevilla sanderi in tissue culture. Four different types of explants (leafy shoot tips, decapitated leafy shoot tips, defoliated shoot tips, decapitated and defoliated shoot tips) were used in the experiment, which were placed on the media vertically, while defoliated shoot tips were placed horizontally or vertically upside down. The explants were cultivated on a Murashige and Skoog (MS) medium supplemented with 1 mg·dm–3 benzyladenine (BA) and 0.5 mg·dm–3 indole-3-butyric acid (IBA). It was noted that both explants orientation and positioning, influenced the multiplication rate. Defoliated shoot tips placed horizontally were characterized by higher multiplication rate (6.8) in comparison to upside down vertical positioning (3.2). It was also observed that removal of shoot apex improved axillary branching, while defoliation of shoots placed in a normal position reduced multiplication rate.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 111-119
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bank genow 'in vitro' - stan obecny
Autorzy:
Zaklukiewicz, K
Sekrecka, D.
Powiązania:
https://bibliotekanauki.pl/articles/836124.pdf
Data publikacji:
1993
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
hodowla roslin
ziemniaki
kultury tkankowe
hodowla in vitro
banki genow
odmiany roslin
plant breeding
potato
tissue culture
in vitro culture
gene bank
plant cultivar
Źródło:
Ziemniak Polski; 1993, 2; 28-36
1425-4263
Pojawia się w:
Ziemniak Polski
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative study on tissue culture induced variation identified with metAFLP and RP – HPLC in barley and triticale regenerants
Autorzy:
Machczynska, J.
Orlowska, R.
Ogorek, K.A.
Bednarek, P.T.
Powiązania:
https://bibliotekanauki.pl/articles/951298.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
tissue culture
in vitro culture
plant regeneration
genetic change
DNA methylation
histone modification
high performance liquid chromatography
barley
triticale
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of PPM upon the sterility of the in vitro cultures in European beech [Fagus sylvatica L.]
Autorzy:
Kraj, W
Dolnicki, A.
Powiązania:
https://bibliotekanauki.pl/articles/56550.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
Plant Preservative Mixture
plant physiology
tissue culture
tree bud
Fagus sylvatica
sterilization
beech
sterility
in vitro
embryo
European beech
bud
Opis:
Investigations were conducted on the strongly infested by microbes embryos isolated from seed stratified in non-sterile conditions or from tree buds. The results showed, that PPM (Plant Preservative Mixture, produced by Plant Cell Technology, Inc.) was very useful for increasing the percentage of sterile cultures disinfected in a 5% solution of NaOCl. The best way of applying PPM turned out to be adding the substance directly to the growing medium. At the concentration of 2 or 4 cm3 x dm-3 of PPM there was an 30% increase in percentage of sterile of in vitro cultures developed from embryos and 70-80% of cultures developed from buds, as compared with the control without PPM. At the range of concentrations employed in this study, no negative effects of PPM upon the development and growth of callus were observed.
Źródło:
Acta Societatis Botanicorum Poloniae; 2003, 72, 4
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Neural modeling of plant tissue cultures: a review
Autorzy:
Zielinska, S.
Kepczynska, E.
Powiązania:
https://bibliotekanauki.pl/articles/81293.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
artificial neural network
biomass
plant tissue
neural model
tissue culture
in vitro condition
micropropagation
radial neural network
neural network
somatic embryo
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The use of nano-silver for disinfection of Pennisetum alopecuroides plant material for tissue culture
Autorzy:
Parzymies, M.
Pudelska, K.
Poniewozik, M.
Powiązania:
https://bibliotekanauki.pl/articles/12683367.pdf
Data publikacji:
2019
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
Pennisetum alopecuroides
grass species
ornamental grass
shoot
explant
plant material
tissue culture
plant growth regulator
micropropagation
contamination
plant regeneration
silver
nanoparticle
disinfection
Opis:
Initiation of tissue culture of many plant species is a very difficult stage due to appearance of many contaminations. The other problem might be a choice of media for regeneration. Initiation of grass species tissue cultures are thought to be very difficult. Therefore, a research was undertaken to evaluate the use of nano-silver particles for plant material disinfection and to estimate a medium Pennisetum alopecuroides. The plant material were buds and nodal explants that were disinfected in 2% NaOCl for 30 min or 0.1% HgCl2 for 1 min. Half of the explants disinfected with NaOCl were soaked in 50, 100 or 250 mg·dm–3 Ag NPs for 1 hour. Explants not soaked in nano-silver were placed on media with Ag NPs at concentrations of 4, 8 or 16 mg·dm–3. An influence of growth regulators on Pennisetum alopecuroides was evaluated in vitro. Regenerated shoots were placed on MS media with: 3 mg·dm–3 BA + 0.3 mg·dm–3 IBA, 3 mg·dm–3 KIN + + 0.3 mg·dm–3 IAA, 1 mg·dm–3 BA + 0.1 mg·dm–3 IBA. It was observed that the use of nano-silver particles lowered the level of contamination. The best results were obtained when Ag NPs was used at concentration of 100–250 mg·dm–3 alone or as a supplementation of the media, at concentration of 4 mg·dm–3 for nodes and 16 mg·dm–3 for adventitious buds. The use of nodal explants allowed to obtain less contamination. Regeneration depended on a media content. The most regenerated shoots were obtained on the MS media supplemented with 1 mg·dm–3 BA and 0.1 mg·dm–3 IBA.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 3; 127-135
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A non caloric sweetener Stevia rebaudiana Bertoni - tissue cultured plantlets for organic farming and home gardening
Autorzy:
Tomaszewska-Sowa, M.
Figas, A.
Sawilska, A.K.
Powiązania:
https://bibliotekanauki.pl/articles/59692.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Stowarzyszenie Infrastruktura i Ekologia Terenów Wiejskich PAN
Tematy:
non-caloric sweetener
sweetener
Stevia rebaudiana
micropropagation
plant growth regulator
tissue culture
plantlet
organic farming
home garden
Źródło:
Infrastruktura i Ekologia Terenów Wiejskich; 2015, IV/3
1732-5587
Pojawia się w:
Infrastruktura i Ekologia Terenów Wiejskich
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Somatic embryogenesis and plant regeneration from hypocotyl and leaf explants of Brassica oleracea var. botrytis (cauliflower)
Autorzy:
Siong, P.K.
Taha, R.M.
Rahiman, F.A.
Powiązania:
https://bibliotekanauki.pl/articles/19314.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
somatic embryogenesis
plant regeneration
hypocotyl
leaf explant
Brassica oleracea var.botrytis
cauliflower
embryogenic callus
callus
tissue culture
Opis:
We investigated direct and indirect formation of somatic embryogenesis in Brassica oleracea var. botrytis (cauliflower), a very important vegetable crop worldwide. Direct somatic embryogenesis, which is rather rare, was achieved in culture of 2-week-old hypocotyl explants of Brassica oleracea var. botrytis on MS medium supplemented with 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5; 1.0; and 1.5 mg/l kinetin. Initial induction of embryogenic callus was achieved on MS supplemented with very low concentrations of 2,4-D (0.05 mg/l and 0.1 mg/l). Indirect somatic embryogenesis from leaf sections was obtained on MS supplemented with 0.05 or 0.1 mg/l 2,4-D. We examined various stages of somatic embryos (globular, heart, torpedo, cotyledonary). More embryos per explant were produced through the indirect pathway (23–25) than through the direct pathway (14–19). The number of embryos produced was high. There is a potential for recurrent, repeated or secondary somatic embryogenesis, possibly an unlimited source for mass propagation and ideal for synthetic seed production in this species. Plant regeneration was achieved on half-strength MS medium without any hormones.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2011, 53, 1
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Withania coagulans (Stocks) Dunal: biotechnological achievements and perspectives
Autorzy:
Teixera da Silva, J.A.
Kher, M.M.
Soner, D.
Nataraj, M.
Powiązania:
https://bibliotekanauki.pl/articles/1928.pdf
Data publikacji:
2015
Wydawca:
Instytut Ogrodnictwa
Tematy:
Withania coagulans
medicinal plant
Solanaceae
shoot regeneration
rooting
in vitro
micropropagation
tissue culture
random amplified polymorphic DNA
Źródło:
Journal of Horticultural Research; 2015, 23, 1
2300-5009
Pojawia się w:
Journal of Horticultural Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of growth regulators on dahlia propagation in tissue culture and acclimatization of plants in ex vitro conditions
Autorzy:
Marcinek, B.
Parzymies, M.
Poniewozik, M.
Kozak, D.
Durlak, W.
Powiązania:
https://bibliotekanauki.pl/articles/12307379.pdf
Data publikacji:
2019
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
plant cultivation
Dahlia
Dahlia x cultorum
dahlia
ornamental plant
flowering plant
tuberous plant
tuberous root
plant acclimatization
growth regulator
cytokinin
gibberellic acid
micropropagation
tissue culture
ex vitro method
microcutting
Opis:
The aim of the work was to evaluate the influence of cytokinins on in vitro propagated dahlia and their consequent effect on acclimatization. Plant material consisted of shoot tips and nodes. Among the three cytokinins, benzyladenine, kinetin and 2-isopentenyl-adenine, only BA effectively stimulated the shoot multiplication from axiliary buds. The highest multiplication rate was obtained from nodes in the presence of 0.25– 0.5 mg·dm–3 BA. Higher concentrations shortened the internodes and decreased the leaf blades and growth of callus. 1 mg·dm–3 of KIN and 2iP positively influenced the shoot growth and size of leaves. Gibberellic acid (GA3) used with BA increased the number of auxillary shoots. The best quality shoots and the highest multiplication rate were obtained when 2 mg·dm–3 BA was used with 5 mg·dm–3 GA3. Cytokinins affected the rooting and acclimatization ex vitro. Dahlia shoots multiplicated in the presence of 1 mg·dm–3 KIN or 2iP rooted faster in the soil and 100% survived in field, while those from 1 mg·dm–3 BA media rooted slowly, had shorter shoots and only 60% of them survived. Plants bloomed after 11–12 weeks in the field. Dahlia plants that had been multiplicated in the presence of KIN had larger diameter and fresh weight in the field. BA and 2iP positively influenced the flower diameter, length of flower stalk and a number of the first-order shoots.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 5; 49-61
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An improved procedure for in vivo and in vitro propagation of Buxus hyrcana, an ornamental shrub critically at risk of extinction
Autorzy:
Negahdar, N.
Hashemabadi, D.
Kaviani, B.
Powiązania:
https://bibliotekanauki.pl/articles/80583.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
ornamental shrub
Buxus hyrcana
endangered plant
plant growth regulator
rooting
stem cutting
tissue culture
indole-3-butyric acid
kinetin
in vitro propagation
in vivo
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2019, 100, 4
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
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