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    Wyświetlanie 1-5 z 5
    Tytuł:
    An automatic system for calculating basic semen parameters
    Autorzy:
    Witkowski, Ł.
    Powiązania:
    https://bibliotekanauki.pl/articles/1965800.pdf
    Data publikacji:
    2004
    Wydawca:
    Politechnika Gdańska
    Tematy:
    image enhancement
    image analysis
    semen
    sperm cell
    cell mobility
    Opis:
    Algorithms for examination of the density and selected parameters of movement of sperm cells have been elaborated and implemented. The conducted research is a part of work on a computer system for semen analysis. The system will allow for an increase in the precision of examination thanks to an exact specification of numerical values of the chosen parameters. An additional advantage of the system is an increased time efficiency of examination. Nowadays, the basic type of examination is an estimated analysis of semen parameters by visual observation of a sample, based on a physician's subjective assessment of an image. Registration of images is impossible in visual analysis.
    Źródło:
    TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk; 2004, 8, 2; 231-236
    1428-6394
    Pojawia się w:
    TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Evaluation of the effects of photostimulation on freeze-thawed bull sperm cells in terms of reproductive potential
    Autorzy:
    Ömür, A.D.
    Powiązania:
    https://bibliotekanauki.pl/articles/16539165.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    in vitro analysis
    laser
    LED (Light Emitting Diode)
    photostimulation
    sperm cell
    Opis:
    The aim of this study was to determine the time-dependent effectiveness of photo-stimulation against bovine sperm cells using a multi-wavelength LED (Light Emitting Diode). Spermatological parameters were evaluated for viability, acrosome structure and motility. In addition, the effect of photo-stimulation on frozen-thawed sperm cells subjected to in vitro capacitation was evaluated in terms of changes in mitochondrial membrane potential. The study consisted of two separate experiments and a total of 32 sperm samples obtained from separate bulls were used. All sperm samples were obtained from Holstein bulls using an artificial vagina. Semen was diluted to a final concentration of 92 x 106 spermatozoa per ml in 0.25 ml straws. The sperm cells were frozen using the conventional method. Straws were kept in a 37°C water bath for 20 seconds and diluted 1:4 in phosphate buffered saline (PBS) to eliminate the potentially deleterious effect of glycerol, the main permeable cryoprotectant in the freezing medium for bull sperm. This dilution also helped in the evaluation of sperm quality parameters. In the first experiment, whereas the 15-10-15 showed no differences with the control, other treatments such as 10-10-10, 5-5-5, and 3-1-3 exhibited significantly higher percentages of viable spermatozoa at 24h. The results obtained for acrosome integrity were pretty much similar to those observed in the sperm viability assessment. In effect, while the treatment consisting of 15-10-15 had no positive effects, shorter treatments exerted a much more positive effect. The percentages of acrosome-intact spermatozoa in 2-1-2 and 1-1-1 were significantly higher than those obtained in the control. The significant differences in mitochondrial membrane potential were observed at 0, 2, 4 and 24h post-photo-stimulation in all treatments, except 15-10-15. The highest increase in the percentage of spermatozoa exhibiting high mitochondrial membrane potential was found in 10-10-10, 5-5-5 and 3-1-3 treatments. With regard to total and progressive motility, whereas 10-10-10 was the best regime, 5-5-5 and 3-1-3 treatments also had a positive effect. However, 15-10-15 appeared to have a stimulating effect upon progressive motility at 2h and 4h but later declined and showed no significant differences with regard to the control at 24h. In the second experiment, not immediately after thawing but after having been kept at room temperature for up to 24h, it was observed that there was no statistical difference in terms of viability, acrosome integrity and total/progressive motility between photostimulation and the control group. This indicates that photo- stimulation is less able to exert a beneficial effect when post-thawed sperm are not immediately stimulated. As a result it was determined that photo-stimulation at a pattern of 10-10-10, 5-5-5, 3-1-3 and, to a lesser extent 2-1-2, increases the resilience of frozen-thawed bull sperm when applied upon thawing.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 2; 249-259
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Examination of the density of semen and analysis of sperm cell movement
    Autorzy:
    Witkowski, Ł.
    Powiązania:
    https://bibliotekanauki.pl/articles/332912.pdf
    Data publikacji:
    2002
    Wydawca:
    Uniwersytet Śląski. Wydział Informatyki i Nauki o Materiałach. Instytut Informatyki. Zakład Systemów Komputerowych
    Tematy:
    poprawa jakości obrazu
    analiza obrazu
    sperma
    plemnik
    mobilność komórek
    image enhancement
    image analysis
    semen
    sperm cell
    cell mobility
    Opis:
    The algorithms for examination of the density and chosen parameters of movement of sperm cell were elaborated and implemented. The conducted research is a part of a work on a computer system for analysis of semen. The system will allow for an increase of the preciseness of examination thanks to the exact specification of numerical values of chosen parameters. Additional advantage of the system is a shorter time of the examination. Nowadays, the basic type of examination is an estimated analysis of parameters of semen done by visual observation of a sample. This type of examination is based on a subjective assessment of an image by a physician. Moreover the registration of images in visual analysis is not possible.
    Źródło:
    Journal of Medical Informatics & Technologies; 2002, 3; MI81-88
    1642-6037
    Pojawia się w:
    Journal of Medical Informatics & Technologies
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Transcriptional activity of Petunia calreticulin gene (PhCRT) involved in pistil transmitting tract maturation, progamic phase and double fertilization
    Autorzy:
    Suwinska, A.
    Lenartowski, R.
    Lenartowska, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/80137.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    double fertilization
    reproductive system
    flowering plant
    sperm cell
    female gametophyte
    homeostasis
    calreticulin
    fluorescent in situ hybridization
    gene expression
    transcriptional activity
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Evaluation of the effect of using mobile phones on male fertility
    Autorzy:
    Wdowiak, A
    Wdowiak, L.
    Wiktor, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/52101.pdf
    Data publikacji:
    2007
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    human health
    sperm cell
    structure
    electromagnetic wave
    male fertility
    male
    mobile telecommunication zob.mobile telephony system
    mobile telephony system
    living organism
    mobile phone
    motility
    Opis:
    The problem of the lack of offspring is a phenomenon concerning approximately 15% of married couples in Poland. Infertility is defi ned as inability to conceive after a year of sexual intercourses without the use of contraceptives. In half of the cases the causative factor is the male. Males are exposed to the effect of various environmental factors, which may decrease their reproductive capabilities. A decrease in male fertility is a phenomenon which occurs within years, which may suggest that one of the reasons for the decrease in semen parameters is the effect of the development of techniques in the surrounding environment. A hazardous effect on male fertility may be manifested by a decrease in the amount of sperm cells, disorders in their mobility, as well as structure. The causative agents may be chemical substances, ionizing radiation, stress, as well as electromagnetic waves. The objective of the study was the determination of the effect of the usage of cellular phones on the fertility of males subjected to marital infertility therapy. The following groups were selected from among 304 males covered by the study: Group A: 99 patients who did not use mobile phones, Group B: 157 males who have used GSM equipment sporadically for the period of 1-2 years, and Group C: 48 people who have been regularly using mobile phone for more than 2 years. In the analysis of the effect of GSM equipment on the semen it was noted that an increase in the percentage of sperm cells of abnormal morphology is associated with the duration of exposure to the waves emitted by the GSM phone. It was also confi rmed that a decrease in the percentage of sperm cells in vital progressing motility in the semen is correlated with the frequency of using mobile phones.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2007, 14, 1
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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