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    Wyświetlanie 1-10 z 10
    Tytuł:
    The effect of cAMP and cGMP on the activity and substrate specificity of protein kinase A from methylotrophic yeast Pichia pastoris.
    Autorzy:
    Frajnt, Magdalena
    Cytryńska, Małgorzata
    Jakubowicz, Teresa
    Powiązania:
    https://bibliotekanauki.pl/articles/1043395.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    protein phosphorylation
    cAMP
    PKA
    cGMP
    yeast
    Opis:
    Cyclic AMP dependent protein kinase (PKA) from Pichia pastoris yeast cells was found to be activated by either cAMP or cGMP. Analogs of cAMP such as 8-chloro-cAMP and 8-bromo-cAMP were as potent as cAMP in PKA activation while N6,2'-O-dibutyryl-cAMP did not stimulate the enzyme activity. It was shown that protamine sulfate was almost equally phosphorylated in the presence of 1-2 × 10-6 M cAMP or cGMP while other substrates such as Kemptide, ribosomal protein S6, were phosphorylated to a lower extent in the presence of cGMP. It was demonstrated that pyruvate kinase is a substrate of PKA which co-purified with the P. pastoris enzyme. Moreover, pyruvate kinase was phosphorylated by PKA in the presence of cAMP and cGMP to comparable levels.
    Źródło:
    Acta Biochimica Polonica; 2003, 50, 4; 1111-1118
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    NH4plus -mediated protein phosphorylation in rice roots
    Autorzy:
    Zhu, X.F.
    Cai, W.H.
    Jung, J.H.
    Xuan, Y.H.
    Powiązania:
    https://bibliotekanauki.pl/articles/19339.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    ammonium
    protein phosphorylation
    rice
    root
    phosphoproteomics
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2015, 57, 2
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Different properties of four molecular forms of protein kinase CK2 from Saccharomyces cerevisiae
    Autorzy:
    Domańska, Katarzyna
    Zieliński, Rafał
    Kubiński, Konrad
    Sajnaga, Ewa
    Masłyk, Maciej
    Bretner, Maria
    Szyszka, Ryszard
    Powiązania:
    https://bibliotekanauki.pl/articles/1041353.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    isoenzyme specificity
    protein phosphorylation
    protein kinase CK2
    yeast
    Opis:
    CK2 is a pleiotropic constitutively active serine/threonine protein kinase composed of two catalytic α- and two regulatory β-subunits, whose regulation is still not well understood. It seems to play an essential role in regulation of many cellular processes. Four active forms of CK2, composed of αα'ββ', α2ββ', α'2ββ', and a free α'-subunit were isolated from wild-type yeast and strains containing a single deletion of the catalytic subunit. Each species exhibits properties typical for CK2, but they differ in substrate specificity and sensitivity to inhibitors. This suggests that each CK2 isomer may regulate different process or may differ in the way of its regulation.
    Źródło:
    Acta Biochimica Polonica; 2005, 52, 4; 947-951
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prp4 kinase is required for proper segregation of chromosomes during meiosis in Schizosaccharomyces pombe
    Autorzy:
    Pozgajova, Miroslava
    Cipak, Lubos
    Trakovicka, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1039511.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Prp4 protein kinase
    S. pombe
    meiosis
    segregation
    protein phosphorylation
    Opis:
    Chromosome segregation during meiosis is a complex process, which leads to production of four haploid gametes from two precursor cells. Reversible phosphorylation of proteins plays a crucial role in this process. The Schizosaccharomyces pombe Prp4 is an essential serine/threonine protein kinase, which belongs to the Clk/Sty family. To study the role of Prp4 in meiosis, we analysed chromosome segregation in a strain carrying conditional analog-sensitive allele of Prp4 protein kinase (prp4-as2). Our data show, that Prp4 protein kinase plays important role in chromosome segregation during meiosis, as revealed by enhanced missegregation of chromosomes in prp4-as2 mutant cells.
    Źródło:
    Acta Biochimica Polonica; 2013, 60, 4; 871-873
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of vanadate on Pichia pastoris growth, protein kinase A activity and ribosomal protein phosphorylation.
    Autorzy:
    Frajnt, Magdalena
    Cytryńska, Małgorzata
    Jakubowicz, Teresa
    Powiązania:
    https://bibliotekanauki.pl/articles/1043701.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    protein phosphorylation
    vanadate
    Pichia pastoris
    PKA
    ribosomal proteins
    protein kinases
    Opis:
    It was found that wild type yeast Pichia pastoris can tolerate vanadate concentration as high as 25 mM in the growth medium. Moreover, four vanadate-resistant P. pastoris strains designated JC100/1, JC100/3, JC100/9 and JC100/15 exhibiting tolerance up to 150 mM vanadate were selected. Growth of P. pastoris was correlated with vanadate to vanadyl reduction and its accumulation in the growth medium. In two selected strains, JC100/9 and JC100/15, protein kinase A activity was much higher in comparison to the wild type strain even without vanadate addition to the growth medium. Moreover, in the presence of vanadate, protein kinase A activity was significantly increased in the wild type and the vanadate-resistant JC100/1 and JC100/3 strains. It was also found that phosphorylation of a 40 kDa protein associated with ribosomes occured in all vanadate-resistant strains from the logarithmic, while in the wild type strain from the stationary growth phase. From the presented results it can be concluded that a protein kinase A signalling pathway(s) might be involved in the mechanism of P. pastoris vanadate resistance. The results also indicate a possible role of the 40 kDa protein in protection of P. pastoris against vanadate toxicity.
    Źródło:
    Acta Biochimica Polonica; 2002, 49, 4; 959-968
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Altered mouse leukemia L1210 thymidylate synthase, associated with cell resistance to 5-fluoro-dUrd, is not mutated but rather reflects posttranslational modification
    Autorzy:
    Cieśla, Joanna
    Frączyk, Tomasz
    Zieliński, Zbigniew
    Sikora, Jacek
    Rode, Wojciech
    Powiązania:
    https://bibliotekanauki.pl/articles/1041288.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    protein phosphorylation
    L1210
    thymidylate synthase
    posttranslational modification
    FdUrd resistance
    Opis:
    Thymidylate synthase purified from 5-fluoro-dUrd-resistant mouse leukemia L1210 cells (TSr) was less sensitive to slow-binding inhibition by 5-fluoro-dUMP than the enzyme from the parental cells (TSp), both enzyme forms differing also in sensitivity to several other dump analogues, apparent molecular weights of monomer and dimer, and temperature dependence of the catalyzed reaction. Direct sequencing of products obtained from RT-PCR, performed on total RNA isolated from the parental and 5-fluoro-dUrd-resistant cells, proved both nucleotide sequences to be identical to the mouse thymidylate synthase coding sequence published earlier (NCBI protein database access no. NP_067263). This suggests that the altered properties of TSr are caused by a factor different than protein mutation, presumably posttranslational modification. As a possibility of rat thymidylate synthase phosphorylation has been recently demonstrated (Samsonoff et al. (1997) J Biol Chem 272: 13281), the mouse enzyme amino-acid sequence was analysed, revealing several potential phosphorylation sites. In order to test possible influence of the protein phosphorylation state on enzymatic properties, endogenous TSp and TSr were purified in the presence of inhibitors of phosphatases. Although both enzyme forms were phosphorylated, as shown by electrophoretical separation followed by phosphoprotein detection, the extent of phosphorylation was apparently similar. However, the same two purified enzyme preparations, compared to the corresponding preparations purified in the absence of phosphatase inhibitors, showed certain properties, including sensitivity to the slow-binding inhibition by FdUMP, altered. Thus properties dependence on phosphorylation was indicated.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 1; 189-198
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    IAA and BAP affect protein phosphorylation-dependent processes during sucrose-mediated G1 to S and G2 to M transitions in root meristem cells of Vicia faba
    Autorzy:
    Polit, J T
    Maszewski, J.
    Rosiak, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/58127.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    Vicia faba
    cell cycle
    sucrose
    auxin
    protein phosphorylation
    cytokinin
    control point
    meristem
    root
    Opis:
    In carbohydrate-starved root meristems of Vicia faba subsp. minor, the expression of two Principal Control Points located at the final stages of the G1 (PCP1) and G2 (PCP2) phases has been found to be correlated with a marked decrease of protein phosphorylation within cell nuclei, nucleoli and cytoplasm. Adopting the same experimental model in our present studies, monoclonal FITC conjugated antibodies that recognize phosphorylated form of threonine (αTPab-FITC) were used to obtain an insight about how the indole-3-acetic acid (IAA), benzyl-6-aminopurine (BAP), and the mixture of both phytohormones influence the time-course changes in an overall protein phosphorylation during sucrose-mediated PCP1→S and PCP2→M transitions. Unsuspectedly, neither IAA, BAP, nor the mixture of both phytohormones supplied in combination with sucrose did up-regulate protein phosphorylation. However using the block-and-release method, it was shown that root meristems of Vicia provided with sucrose alone indicated higher levels of αTPab-FITC. Contrarily, phytohormones supplied in combination with sucrose induced apparent decline in phosphorylation of cell proteins, which - when compared with the influence of sucrose alone - became increasingly evident in time. Thus, it seems probable, that a general decline in the amount of αTPab-FITC labeled epitopes may overlay specific phosphorylations and dephosphorylations governed by the main cell cycle kinases and phosphatases.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2004, 73, 1
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of phosphorylation and succinilation of yeast protein on its enzymatic digestibility and functional properties
    Wpływ fosforylacji i sulecynylacji białka drożdżowego na jego strawność enzymatyczną i właściwości funkcjonalne
    Autorzy:
    Giec, A.
    Stasińska, B.
    Lampart-Szczapa, E.
    Skupin, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/1399535.pdf
    Data publikacji:
    1989
    Wydawca:
    Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
    Tematy:
    protein of the yeast Torula utilis
    protein phosphorylation
    protein succinilation
    functional properties
    enzymatic digestibility
    Opis:
    It was found that succinilation and phosphorylation of protein lead to its greater yield from yeast compared to the traditional alkaline extraction, causing a more effective reduction of nucleic acids content in the final product and a considerable improvement of the functional properties of isolated substances. At the same time phosphorylation caused a smaller reduction of enzymatic digestibility of the protein accompanying all the advantageous effects.
    Włączenie białka mikrobiologicznego do żywności wymaga dostosowania jego właściwości fizykochemicznych. W literaturze opisano wiele metod osiągnięcia tego celu dzięki różnym modyfikacją chemicznym. Brak jest analizy porównawczej ich skuteczności oraz wpływu na wartość biologiczną uzyskanych preparatów białkowych. Celem niniejszej pracy było porównanie efektywności zabiegów sulecynylacji i fosforylacji białka drożdżowego z tradycyjną estrakcją alkaliczną po hydrolizie kwa sów nukleinowych. Badania wykazały że proces sulecynylacji oraz fosforylacji zwiększa ekstraktywność białka wskutek przesunięcia punktu izoelektrycznego, co wpływa istotnie na obniżenie zawartości kwasów nukleinowych w produkcie o ok. 60 % i poprawę właściwości funkcjonalnych (tabela). Fosforylacja w znacznie mniejszym topniu obniżyła strawność enzymatyczną preparatu niż sulecynylacja, a dodatkowo z uwagi na swą odwracalność wydaje się być godną polecenia metodą uzyskiwania z homogenatów drożdżowych białka przeznaczonego do żywności.
    Źródło:
    Acta Alimentaria Polonica; 1989, 15(39), 1; 63-66
    0137-1495
    Pojawia się w:
    Acta Alimentaria Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    SDS/PAGE characteristics of protein kinases tightly associated with chick embryo brain ribosomes
    Autorzy:
    Sanecka-Obacz, Maria
    Powiązania:
    https://bibliotekanauki.pl/articles/1044449.pdf
    Data publikacji:
    1999
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    protein phosphorylation
    chick brain development
    ribosomal kinases
    CK2
    PKA
    CK1
    fetal brain
    brain ribosomes
    Opis:
    Protein kinases tightly associated with chick embryo brain ribosomes washed with Triton X-100 and KCl were characterized by their ability to phosphorylate ribosomes and two exogenous substrates, histone IIA and casein. c-AMP-dependent kinase (PKA) and casein kinases (CK1, CK2) were examined in the presence of specific modulators by SDS/ PAGE followed by  renaturation in gel assay according to Kameshita & Fujisawa (Anal. Biochem. 1989, 183, 139-143). Basing on these data it can be presumed that PKA activity increases, but the levels of CK2 and CK1 decrease during chick embryo development.
    Źródło:
    Acta Biochimica Polonica; 1999, 46, 4; 911-917
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Catalytic activity of mutants of yeast protein kinase CK2α
    Autorzy:
    Sajnaga, Ewa
    Kubiński, Konrad
    Szyszka, Ryszard
    Powiązania:
    https://bibliotekanauki.pl/articles/1040685.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    protein phosphorylation
    ATP binding
    mutagenesis
    protein kinase CK2
    ATP-competitive inhibitors
    Saccharomyces cerevisiae
    spermine
    heparin
    yeast
    Opis:
    Yeast CK2 is a highly conserved member of the protein kinase CGMC subfamily composed of two catalytic (α and α') and two regulatory (β and β') subunits. The amino-acid sequences of both catalytic subunits are only 60% homologous. Modelling of the tertiary structure of the CK2α displays additional α-helical structures not present in the CK2α' subunit, connecting the ATP-binding loop with the catalytic and activation loops. Deletion of this part causes drastic structural and enzymatic changes of the protein (CK2αΔ91-128) with characteristics similar to yeast CK2α' (low sensitivity to salt, heparin and spermine). Additionally, the deletion causes an over 5-fold decrease of the binding affinity for ATP and ATP-competitive inhibitors (TBBt and TBBz). The structural basis for TBBt and TBBz selectivity is provided by the hydrophobic pocket adjacent to the ATP/GTP binding site, which is smaller in CK2 than in the majority of other protein kinases. The importance of hydrophobic interactions in the binding of specific inhibitors was investigated here by mutational analysis of CK2α residues whose side chains contribute to reducing the size of the hydrophobic pocket. Site-directed mutagenesis was used to replace Val67 and Ile213 by Ala. The kinetic properties of the single mutants CK2αVal67Ala and CK2αIle213Ala, and the double mutant CK2Val67Ala Ile213Ala were studied with respect to ATP, and both inhibitors TBBt and TBBz. The Km values for ATP did not change or were very close to those of the parental kinase. In contrast, all CK2α mutants analysed displayed higher Ki values towards the inhibitors (10 to 12-fold higher with TBBt and 3 to 6-fold with TBBt) comparing to recombinant wild-type CK2α.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 4; 767-776
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-10 z 10

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