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Wyszukujesz frazę "Anaplasma" wg kryterium: Wszystkie pola

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    Wyświetlanie 1-6 z 6
    Tytuł:
    Wild game as a reservoir of Anaplasma phagocytophilum in North-Western Poland
    Autorzy:
    Adamska, M
    Skotarczak, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/840225.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasite
    Polska
    reservoir
    roe deer
    bacteria
    Anaplasma phagocytophilum
    polymerase chain reaction
    intracellular parasite
    tick
    Źródło:
    Annals of Parasitology; 2007, 53, 2
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wild game as a reservoir of Anaplasma phagocytophilum in North-Western Poland
    Autorzy:
    Adamska, M.
    Skotarczak, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143862.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasite
    Polska
    reservoir
    roe deer
    bacteria
    Anaplasma phagocytophilum
    polymerase chain reaction
    intracellular parasite
    tick
    Opis:
    Background. The aim of the study was to determine the role of game animals as reservoirs of Anaplasma phagocytophilum, a bacteria species transmitted by Ixodes ricinus ticks, from north−western Poland (Zachodniopomorskie vovoidship). The area under question is endemic for A. phagocytophilum. Material and methods. Blood and spleen samples were taken from 72 roe deer between April and December 2003. Animals culled during winter did not harbour ticks, on the other hand 155 individuals of Ixodes ricinus were collected from 35 of 43 animals taken during spring. We tested all samples for A. phagocytophilum by PCR amplification of the msp2 gene. An individual was considered infected if pathogens were detected in at least one isolate (blood or a tissue sample). Results. DNA from A. phagocytophilum was not found in isolates from ticks collected from the animals. The general level of infection for the roe deer was 31.94% (23/72). DNA of A. phagocytophilum was most commonly detected in blood samples; only in three cases was anaplasma DNA detected in spleen and not in blood. Ruminants seem to be the most competent reservoir for A. phagocytophilum in north−western Poland.
    Źródło:
    Wiadomości Parazytologiczne; 2007, 53, 2; 103-107
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Serological and molecular survey of Anaplasma phagocytophilum in Italian hunting dogs
    Autorzy:
    Ebani, V.V.
    Bertelloni, F.
    Turchi, B.
    Cerri, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/50930.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    serological survey
    molecular survey
    Anaplasma phagocytophilum
    hunting dog
    dog
    polymerase chain reaction
    indirect immunofluorescence antibody test
    tick
    zoonotic pathogen
    Italy
    prevalence
    Opis:
    The presence of Anaplasma phagocytophilum, a tick-transmitted zoonotic pathogen, has been reported in Italy in humans and several animal species, including dogs, but data concerning its prevalence in the Italian canine population are lacking. The aim of this study was to determine the prevalence of A. phagocytophilum infection in hunting dogs which live in geographic areas of central Italy where the infection had been previously detected in wild animals. Sera from 215 hunting dogs were tested by indirect immunofluorescence antibody test (IFAT) to detect antibodies to A. phagocytophilum. Buffy coat samples from the same 215 animals were submitted to a polymerase chain reaction (PCR) assay specific for the A. phagocytophilum 16S rRNA gene. Thirty-two (14.8%) dogs proved to be seropositive with antibody titres from 1:40–1:2,560. The highest seroprevalence was observed in dogs 6–10-year-old. Two seropositive (0.9%) animals were also PCR positive. Sequencing of PCR products revealed gene sequences of A. phagocytophilum in both cases. These results confirm the presence of A. phagocytophilum in the Italian areas studied; thus, the zoonotic potential of this agent should be considered particularly for people, as hunters, at risk of exposure to tick bites.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prevalence of Anaplasma phagocytophilum in Ixodes ricinus ticks determined by polymerase chain reaction with two pairs of primers detecting 16S rRNA and ankA genes
    Autorzy:
    Chmielewska-Badora, J
    Zwolinski, J.
    Cisak, E.
    Wojcik-Fatla, A.
    Buczek, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51023.pdf
    Data publikacji:
    2007
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Lublin region
    Polska
    pathogen
    Ixodes ricinus
    Eastern Poland
    tick
    determination
    animal pathogen
    human pathogen
    ankA gene
    16S rRNA gene
    granulocytic anaplasmosis
    bacteria
    Anaplasma phagocytophilum
    polymerase chain reaction
    Opis:
    A total of 684 Ixodes ricinus ticks (321 nymphs, 184 males, and 179 females) were collected by fl agging lower vegetation in 6 forest districts located on the territory of Lublin province (eastern Poland). Ticks were examined by polymerase chain reaction (PCR) method for the presence of Anaplasma phagocytophilum DNA with two pairs of primers: EHR521/EHR747 for detecting 16S rRNA gene, and LA6/LA1 for detecting ankA gene. To study the relationship between infection in ticks and people occupationally exposed to tick bite, blood serum samples of 261 forestry workers employed in the same forest districts were examined by immunofl uorescence method for the presence of specifi c antibodies against A. phagocytophilum. A total of 70 ticks out of 684 examined (10.2%) showed the presence of A. phagocytophilum 16S rRNA gene. The prevalence of infection was signifi cantly dependent on tick’s stage (χ2=49.2, p<0.00001) and geographical locality (χ2=34.4, p<0.00001). The percentage of I. ricinus females infected with A. phagocytophilum (24.6%) was signifi cantly greater compared to males (6.5%) and nymphs (4.4%) (p<0.00001). Only 19 ticks out of 684 examined (2.8%) showed the presence of A. phagocytophilum ankA gene, signifi cantly less compared to 16S rRNA gene (p<0.00001). The prevalence of infection demonstrated by the presence of ankA gene was also signifi cantly dependent on tick’s stage (χ2=23.6, p<0.00001) but not on locality (χ2=9.8, p=0.082). A signifi cant correlation was found between the presence of A. phagocytophilum 16S rRNA gene in I. ricinus female ticks from the particular forest districts and the serologic response to A. phagocytophilum of forestry workers employed in the same districts (p<0.05). No signifi cant correlation was found between the presence of A. phagocytophilum ankA gene in I. ricinus ticks and serologic response of exposed workers. In conclusion, detection of A. phagocytophilum infection in ticks by PCR with the use of EHR521/EHR747 primers detecting 16S rRNA gene is signifi cantly more sensitive compared to LA6/LA1 primers detecting ankA gene.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2007, 14, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of the piroplasms isolated from horses with clinical piroplasmosis in Poland
    Identyfikacja piroplazm izolowanych od koni w Polsce w klinicznych przypadkach choroby
    Autorzy:
    Adaszek, L.
    Gorna, M.
    Krzysiak, M.
    Adaszek, M.
    Garbal, M.
    Winiarczyk, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/838784.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Anaplasma
    Babesia
    Ehrlichia
    Polska
    Theileria
    animal disease
    disease symptom
    diseased animal
    horse
    identification
    piroplasm
    piroplasmosis
    polymerase chain reaction
    ricketsia
    treatment
    tick-borne disease
    Źródło:
    Annals of Parasitology; 2011, 57, 1
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of the piroplasms isolated from horses with clinical piroplasmosis in Poland
    Identyfikacja piroplazm izolowanych od koni w Polsce w klinicznych przypadkach choroby
    Autorzy:
    Adaszek, L.
    Górna, M.
    Krzysiak, M.
    Adaszek, M.
    Garbal, M.
    Winiarczyk, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143301.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Anaplasma
    Babesia
    Ehrlichia
    Polska
    Theileria
    animal disease
    disease symptom
    diseased animal
    horse
    identification
    piroplasm
    piroplasmosis
    polymerase chain reaction
    ricketsia
    treatment
    tick-borne disease
    Opis:
    The study was aimed at determining the cause of the diseases in three horses exhibiting symptoms of fever, ataxia, mucus membrane paleness, haematuria and thrombocytopenia. The PCR technique revealed the presence in the blood of 18S RNA Babesia/Theileria spp. genetic material. DNA amplification using primers RLB F2 and RLB R2 produced 430 bp size products. The sequences of these PCR products demonstrated a 95.6–97.5% similarity with the sequence of the fragment of 18S RNA Babesia equi, gene number DQ287951 in the GenBank. The treatment utilizing the subcutaneous application of the imidocarb resulted in gradual recovery of the diseased animals.
    Źródło:
    Wiadomości Parazytologiczne; 2011, 57, 1; 21-26
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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