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Wyszukujesz frazę "callus culture" wg kryterium: Temat

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    Wyświetlanie 1-5 z 5
    Tytuł:
    Artemisia species in vitro cultures for production of biologically active secondary metabolites
    Autorzy:
    Grech-Baran, M.
    Pietrosiuk, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/80902.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Artemisia
    in vitro culture
    hairy root
    bioreactor
    natural drug
    arglabin
    artemisinin
    plant regeneration
    callus culture
    shoot culture
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2012, 93, 4
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Plant regeneration from immature inflorescence-derived callus of Italian ryegrass [Lolium multiflorum Lam.]: chromosome number and fertility of regenerated plants
    Autorzy:
    Zwierzykowski, Z
    Rybczynski, J.J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2044256.pdf
    Data publikacji:
    1998
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    chromosome structure
    plant regeneration
    female fertility
    callus culture
    chromosome number
    meiotic behaviour
    Italian rye-grass
    breeding method
    callus induction
    plant breeding
    Lolium multiflorum
    immature inflorescence
    Opis:
    Twenty field-grown genotypes of diploid Italian ryegrass (Lolium multiflorum Lam., 2n = 2x = 14) were tested for their ability to induce callus and regenerate plants. Callus cultures were initiated from segments of immature inflorescences cultured on the MS medium supplemented with 4.0 mg L⁻¹ 2,4-D. The calluses were subcultured first on the maintaining medium (MS medium with 2.0 mg L⁻¹ 2,4-D) and later on the rooting medium (MS medium with 0.2 mg L⁻¹ 2,4-D). The frequency of callus induction varied depending on the source of explant and the initial genotype. A total of 473 green plantlets were regenerated, of which 420 were established in the soil. All these plants had the morphological characteristics of Italian ryegrass. Among 372 regenerants analysed cytologically, 302 (81.2%) had the expected diploid chromosome number (2n = 2x= 14), 65 (17.5%) were tetraploid (2n = 4x = 28); several aneuploids and mixoploids were also observed. All diploid and tetraploid regenerants were male and female fertile. However, a great variation of female fertility within and between both groups of regenerants was observed.
    Źródło:
    Journal of Applied Genetics; 1998, 39, 3; 217-224
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Somatic embryogenesis and plant regeneration from hypocotyl and leaf explants of Brassica oleracea var. botrytis (cauliflower)
    Autorzy:
    Siong, P.K.
    Taha, R.M.
    Rahiman, F.A.
    Powiązania:
    https://bibliotekanauki.pl/articles/19314.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    somatic embryogenesis
    plant regeneration
    hypocotyl
    leaf explant
    Brassica oleracea var.botrytis
    cauliflower
    embryogenic callus
    callus
    tissue culture
    Opis:
    We investigated direct and indirect formation of somatic embryogenesis in Brassica oleracea var. botrytis (cauliflower), a very important vegetable crop worldwide. Direct somatic embryogenesis, which is rather rare, was achieved in culture of 2-week-old hypocotyl explants of Brassica oleracea var. botrytis on MS medium supplemented with 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5; 1.0; and 1.5 mg/l kinetin. Initial induction of embryogenic callus was achieved on MS supplemented with very low concentrations of 2,4-D (0.05 mg/l and 0.1 mg/l). Indirect somatic embryogenesis from leaf sections was obtained on MS supplemented with 0.05 or 0.1 mg/l 2,4-D. We examined various stages of somatic embryos (globular, heart, torpedo, cotyledonary). More embryos per explant were produced through the indirect pathway (23–25) than through the direct pathway (14–19). The number of embryos produced was high. There is a potential for recurrent, repeated or secondary somatic embryogenesis, possibly an unlimited source for mass propagation and ideal for synthetic seed production in this species. Plant regeneration was achieved on half-strength MS medium without any hormones.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2011, 53, 1
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of genetic and environmental factors on anther culture response of wheat
    Autorzy:
    Zhou, H
    Konzak, C F
    Powiązania:
    https://bibliotekanauki.pl/articles/2046623.pdf
    Data publikacji:
    1997
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    callus response
    plant regeneration
    environmental factor
    doubled haploid
    wheat
    genetic factor
    Triticum aestivum
    anther culture
    Opis:
    The influences of genetic and environmental factors on the anther culture responses of wheat were investigated. Significant differences for callus induction, plant regeneration, and green plant percentages were observed when the nucleus of Triticum acstivum L. cv. Selkirk was transferred to ten alien cytoplasms by substitution backcrosses. In most cases, the alien cytoplasms decreased anther culture responses, but sometimes they were as good as or better than the T. aestivum cytoplasm. Significant within-genotype variation for anther culture responses were observed for wheat varieties Chris, Yecora Rojo, WA7176 and Edwall, indicating genetic heterogeneity in the present commercial cultivars, and potential for improving anther culture responses by in vitro prescreening. When five genotypes (Chris, Pavon 76, Butte 86, WA6916, and Edwall) were cultured across three (potato-4 liquid, 100 g L⁻¹ ficoll-supplemented, and 6 g L⁻¹ agar-solidified) induction media, the liquid and ficoll-containing media were 10 to 15 times more productive than the agar-solidified medium. Whereas, the ficoll medium was not significantly different from the liquid medium. Several low concentration starch media appeared promising to replace current induction media. The starch media sustained the highcallus-induction properties of the liquid medium, while improving callus aeration similar to that observed on solid media, resulting in markedly higher plant regeneration and green plant percentages.
    Źródło:
    Journal of Applied Genetics; 1997, 38, 4; 393-406
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro morphogenic events in culture of Lotus corniculatus L. seedling root explants
    Autorzy:
    Rybczynski, J J
    Karolkowska, M.
    Kaczmarek, Z.
    Mikula, A.
    Fiuk, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/57212.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    in vitro culture
    morphogenic event
    Lotus corniculatus
    seedling root explant
    plant regeneration
    cytokinin
    callus proliferation
    shoot differentiation
    root explant
    Opis:
    The experiments were carried out on Lotus corniculatus (L.) seedling root explants of the cultivar varieties Skrzeszowicka, Caroll A10 and strain 175. Callus formation and shoot regeneration were the major explant response depended mainly on of the studied genotype and used plant growth regulators (PGRs). Primary cortex of proximal and distal end of explant was the most active tissue for callus proliferation. For shoot primordia differentiation deeper zones of cortex took a part. The process of meristematic centre initiation was not uniform and various level of shoot differentiation events were observed not earlier than 3 weeks of culture. Usually, the shoot primordia regeneration began on proximal rather than distal end of the explant. BAP rather than urea derivatives stimulated shoot proliferation in extended cultures. Increasing of BAP and TDZ concentrations brought about the explant polarity and expansion of the meristematic zones. The explant position in root did not have significant influence on the number of regenerated shoots. The cultures only had better bud formation by TDZ when compared to BAP. BAP stimulated bud formation and development of the shoots from them. Short term of TDZ treatment of explants stimulated meristem formation which developed into buds and shoots. CPPU stimulated callus proliferation and bud formation when explants pretreatment was prolonged from 12 to 36 hrs.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2006, 75, 3; 191-200
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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