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Wyszukujesz frazę "Kosińska, A." wg kryterium: Autor


Wyświetlanie 1-2 z 2
Tytuł:
Effect of plant growth regulators and explant types on induction and growth of callus of Primula veris L.
Wpływ regulatorów wzrostu i rodzaju eksplantatów na indukcję kalusa i wzrost pierwiosnka lekarskiego (Primula veris L.)
Autorzy:
Morozowska, M.
Wesolowska, M.
Glowicka-Woloszyn, R.
Kosinska, A.
Powiązania:
https://bibliotekanauki.pl/articles/72672.pdf
Data publikacji:
2013
Wydawca:
Instytut Włókien Naturalnych i Roślin Zielarskich
Tematy:
plant growth
growth regulator
explant type
callus induction
growth
callus
Primula veris
proliferation
Opis:
Primula veris L. (Primulaceae) is a well-known medicinal herb. The callus induction response of three explant types: roots, cotyledons, and hypocotyls of four-week-old cowslip seedlings were evaluated. The highest statistically different callus induction rate was 93.6% and was obtained from root explants on MS medium supplemented with 0.1 mg/l BA and 5.0 mg/l PIC. Calli also appeared on 83.3% of cotyledon explants on MS medium supplemented with 1.0 mg/l BA and 3.5 mg/l 2,4-D and on 81.0% of root explants on MS medium supplemented with 0.1 mg/l KIN and 2.0 mg/l 2,4-D. These values were not statistically different. The average time required for callus initiation was 4 to 6 weeks, however, it depended on the explants type. The most suitable condition for callus proliferation and growth was MS medium with 0.5 mg/l TDZ and 0.1 mg/l NAA, and with 1.0 mg/l BA and 2.0 mg/l or 3.5 mg/l 2,4-D. No light conditions proved to be more favourable for cowslip calli induction and growth.
Primula veris L. (Primulaceae) to znana roślina lecznicza. Opracowano metodę otrzymywania in vitro tkanki kalusowej z trzech rodzajów eksplantatów: korzeni, liścieni i hypokotyli z czterotygodniowych siewek pierwiosnka lekarskiego. Najwyższy, istotny statystycznie i wynoszący 93,6% indukcji tkanki kalusowej uzyskano z eksplantatów korzeniowych na podłożu Murashige i Skooga (MS) zawierającego 0,1 mg/l BA i 5,0 mg/l PIC. Indukcję kalusa obserwowano także na 83,3% eksplantatów liścieniowych na pożywce MS uzupełnionej 1,0 mg/l BA i 3,5 mg/l 2,4-D oraz na 81,0% eksplantatów korzeniowych na pożywce MS zawierającej 0,1 mg/l KIN i 2,0 mg/l 2,4-D. Średni czas potrzebny do inicjacji kalusa wyniósł 4 do 6 tygodni i zależał od rodzaju eksplantatu. Podłoża MS uzupełnione 0,5 mg/l TDZ i 0,1 mg/l NAA oraz 1,0 mg/l BA i 2,0 mg/l lub 3,5 mg/l 2,4-D okazały się najodpowiedniejszymi dla dalszego rozwoju i wzrostu zregenerowanej tkanki kalusowej. Warunki bez dostępu światła były korzystniejsze dla indukcji i wzrostu tkanki kalusowej pierwiosnka lekarskiego.
Źródło:
Herba Polonica; 2013, 59, 3
0018-0599
Pojawia się w:
Herba Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phenolic compounds and properties of antioxidants in grapevine roots [Vitis vinifera L.] under low-temperature stress followed by recovery
Autorzy:
Weidner, S
Kordala, E.
Brosowska-Arendt, W.
Karamac, M.
Kosinska, A.
Amarowicz, R.
Powiązania:
https://bibliotekanauki.pl/articles/57473.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
oxidative stress
environment condition
plant growth
root
Vitis vinifera
phenolic compound
chill stress
plant development
grape-vine
antioxidative system
Opis:
The research has been performed on roots of Vitis vinifera, cv. Himrod, obtained from seedlings grown under chill stress conditions (+10oC in the day and +7oC at night), under optimum conditions (+25oC in the day and +18oC at night) and from seedling which underwent a recover period after the chill stress treatment. The purpose of the study has been to determine quantitative and qualitative changes in phenolic compounds as well as to demonstrate changes in antiradical properties of extracts from grapevine roots, which appeared as a result of chill stress and during recovery under the optimum conditions following the stress. Phenolic compounds from grapevine roots were extracted using 80% acetone. The total content of phenolics was determined by colorimetry. The content of tannins was tested by precipitation with bovine serum albumin. The reducing power as well as DPPH• free radical and ABTS+• cation radical scavenging activity of the extracts were also tested. In order to identify phenolic compounds present in the extracts the RP-HPLC technique was employed. The tested material was found to contain tannins and three identified phenolic acids: ferulic, caffeic and p-coumaric ones. The latter occurred in the highest concentrations (from 4.46 to 6.28 µg/g fresh matter). Ferulic acid appeared in smaller amounts (from 1.68 to 2.65 µg/g fresh matter), followed by caffeic acid (from 0.87 to 1.55 µg/g fresh matter). Significantly less total phenolic compounds occurred in roots of seedlings subjected to chill stress. However, the total content of these compounds increased significantly in roots of plants which underwent recovery after chill stress. Concentration of tannins was determined by two methods. The content of condensed tannins was depressed in roots as a result of low temperature stress, whereas the content of condensed and hydrolysing tannins (determined via the BSA method) rose under chill stress conditions. A significant increase in tannins in root extracts (determined with both methods) was found during the recovery process after the stress. The three identified phenolic acids appeared in grapevine roots as ester-bound compounds. It has been demonstrated that the content of phenolic acids significantly fell as a result of low temperatures, but increased during recovery after chill stress. The weakest ability to scavenge DPPH• and ABTS+• free radicals as well as the reducing power were shown by the extract obtained from grapevine roots from the seedlings subjected to chill stress. Both free radical scavenging activity and reducing power were observed to increase considerably during recovery after stress. This seems to prove that during the recovery process following chill stress the synthesis of antioxidative compounds in grapevine roots is much more intensive.
Źródło:
Acta Societatis Botanicorum Poloniae; 2009, 78, 4; 279-286
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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