Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

English (EN)·Polski (PL)·Yкраїнський (UK)
Przejdź do strony domowej biblioteki Centralna Biblioteka Wojskowa Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaCentralna Biblioteka Wojskowa

Wyszukujesz frazę "giardia" wg kryterium: Temat

  Lista filtrów
Wyrównaj
Wyświetlanie 1-15 z 16
Tytuł:
Wystepowanie gatunkow i genotypow Giardia u ludzi i zwierzat w Wielkopolsce
Occurrence of Giardia species and genotypes in humans and animals in Wielkopolska region, Poland
Autorzy:
Solarczyk, P.
Powiązania:
https://bibliotekanauki.pl/articles/837534.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Wielkopolska
wystepowanie
pasozyty jelitowe
pasozyty zwierzat
genotyp
Giardia
pierwotniaki
gatunki
pasozyty czlowieka
Źródło:
Annals of Parasitology; 2009, 55, 4; 459-462
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Występowanie gatunków i genotypów Giardia u ludzi i zwierząt w Wielkopolsce
Occurrence of Giardia species and genotypes in humans and animals in Wielkopolska region, Poland
Autorzy:
Solarczyk, P.
Powiązania:
https://bibliotekanauki.pl/articles/2143568.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Wielkopolska
wystepowanie
pasozyty jelitowe
pasozyty zwierzat
genotyp
Giardia
pierwotniaki
gatunki
pasozyty czlowieka
Opis:
Giardia is the most common intestinal protozoan parasite found in humans and animals worldwide. Although it has been known for three hundred years, the nomenclature, taxonomy, host specificity, and pathogenicity of Giardia still arouse numerous controversies and ambiguities. Giardia is classified into six species, that are characterised by various ranges of hosts. The most dubious species is G. intestinalis, which includes a dozen or so genotypes, and only two of them (genotype A and B) have wide ranges of hosts, including humans. Moreover, in some genotype assemblages of G. intestinalis certain subgenotypes were distinguished and it was proven that in the same host species various subgenotypes of this parasite may occur. Bearing in mind the significant genetic heterogeneity of G. intestinalis and the fact that various genotypes and subgenotypes of this parasite are characterised by the broad or narrow host specificity, the data concerning the frequency of giardiosis occurrence are insufficient. It is necessary to use molecular biology techniques in order to define the genotype and/or the subgenotype of G. intestinalis that are found in humans and in certain animal species. Furthermore, since more and more pieces of evidence connected with a possibility of the sexual recombination of Giardia are gathered, it is unknown if genotypes and subgenotypes of this parasite are stable in time. The aim of this thesis was to define the frequency of Giardia occurrence in humans and animals in Wielkopolska region, to identify species and genotypes of Giardia that occur in humans and animals, as well as to obtain an axenic culture of the chosen isolates of Giardia from animals and to compare the sequence of the b-giardin gene fragment obtained from the DNA isolated from cysts and trophozoites in order to check if the axenisation of G. intestinalis leads to the selection of genotypes or if Giardia genotypes are stable in time. Altogether, 2183 faecal samples were examined for the presence of Giardia cysts; 447 faecal samples were taken from 232 persons coming from 5 cities situated in Wielkopolska, and 1736 faecal samples were obtained from 123 animal species, including: 266 faecal samples from 113 species of animals kept in the Zoological Garden in Poznań, 1286 samples from 4 species of breeding animals, 118 samples from dogs, and 66 samples from 5 species of wild animals. Faecal samples were taken from animals coming from 25 places in Wielkopolska. Moreover, seven isolates of G. intestinalis were used in the studies, which were obtained from various species of hosts and kept in an axenic in vitro culture. Microscopic, molecular and bio-informative methods were used in the studies. From each faecal sample fresh smears were made in a 0.6% solution of physiological salt and in Lugol’s solution, as well as a permanent smear stained with trichrome was made. Moreover, the following molecular techniques were implemented in the studies: DNA extraction and purification, the PCR technique (two molecular markers), electrophoresis and visualisation of PCR products, and sequencing. A fragment of the b-giardin gene was used as a molecular marker in order to define the genotype and subgenotype of Giardia. Only in the case of genotyping of two Giardia isolates obtained from Peromyscus eremicus another molecular marker (SSU rRNA)was additionally used. Some widely available computer programmes (Chromas, CAP 3, BioEdit, BLASTn, MEGA version 4.0) were utilised in the analysis of the sequence of the b-giardin gene fragment and in the phylogenetic analysis. The culture of Giardia trophozoites was established to compare the sequence of the partial b-giardin gene from cysts and trophozoites. Concentration and purification of Giardia cysts in the saccharose gradient, and the excystation technique were applied in the studies to obtaining an axenic in vitro culture. In this study, Giardia cysts were found in 12 faecal samples obtained from 3 persons and 5 animal species. Giardia cysts were found only in faecal samples from humans living in Poznań and the samples obtained from animals coming from Poznań and around Puszczykowo. The highest frequency of infection was stated in domestic animals (2.5%) and in animals kept in the Zoological Garden (2.0%), whereas a slightly lower frequency was noticed in wild animals (1.5%) and in humans (1.3%). No Giardia cysts were found in the faecal samples collected from breeding animals. Two new species of Giardia hosts were identified, namely Rhinella marina and Peromyscus eremicus; however, due to a minimal amount of faecal samples supplied for the study it was impossible to define the species and genotype of this parasite. PCR products (the partial of b-giardin gene) were obtained in seven faecal samples out of the ten studied, including three samples from people and four faecal samples derived from three animal species (i.e. dog, tamandua, red deer). Moreover, molecular characterization of seven Giardia isolates from three persons and four animal species (red-bellied monkey, silver marmoset, Thomson’s gazelle, and sheep) kept in an axenic in vitro culture was performed. Based on the b-giardin sequence fragment analysis, four assemblages of G. intestinalis genotypes were identified (A, B, C and D). In humans, A and B G. intestinalis genotypes and three subgenotypes, including a cosmopolitan subgenotype A2 and two new subgenotypes A and B were detected. Furthermore, four G. intestinalis genotypes were found in animals, including three genotypes which are non-infectious to humans, namely: genotypes C and D in dogs and a cervids-specific genotype A in red deer (Cervus elaphus), which indicate that these animals do not constitute the source of infection to humans. On the other hand, in a tamandua from the Zoological Garden in Poznań a new subgenotype B of G. intestinalis was identified, which due to a close relationship with Giardia isolates obtained from humans is potentially infectious to man. In none of the studied faecal samples a mixed infection of Giardia was found. To date, nine sequences of the partial b-giardin gene have been deposited in the National Center for Biotechnology Information (NCBI), including two sequences of Giardia isolates obtained from humans (GenBank accession numbers FJ009207, FJ009208), three sequences of isolate obtained from red deer (GenBank accession numbers EU621373, EU626198, EU216429), two sequences of both Giardia isolates obtained from dogs (GenBank accession numbers FJ009205, FJ009206), and the single sequences obtained from tamandua (GenBank accession number FJ009209) and from Thomson’s gazelle (GenBank accession number EU626199). According to the literature, an axenic in vitro culture of G. intestinalis was obtained from a red deer for the first time. Based on the analysis of the sequence of the b-giardin gene fragment obtained from the DNA isolated from cysts and trophozoites it was proven that the red deer was infected with a single population of Giardia and that during the axenisation of the culture no mutation in the DNA of the parasite’s trophozoites took place. Probably the time distance that the DNA was isolated from the trophozoites kept in the culture was too short to cause the mutation. This suggestion is confirmed by the results of the genotyping of seven G. intestinalis isolates obtained from various host species and kept in an axenic in vitro culture for at least a number of years. Based on the molecular characteristics it was stated that all the studied isolates from the axenic culture were identical and belonged to the same assemblage B. The comparision with the sequences from GenBank database revealed that all mentioned isolates were 99% similar to the sequence of Giardia Nij5 isolate obtained from a person from the Netherlands and characterised as genotype B1. Due to the sameness of the molecular marker sequences it seems improbable that the identical G. intestinalis genotype occurred in various time periods (the largest difference was 14 years) in humans and in a number of animal species in diverse areas of Wielkopolska region. Quite opposite, the long-term keeping of these isolates in the homogenous conditions of an axenic in vitro culture leads to the selection of a genotype or proves the instability of genotypes of this parasite. Long-term studies need to be conducted in order to verify these hypothesis. Their results will have a key meaning in explaining the genetic structure of the Giardia population and in understanding the molecular epidemiology of giardiosis.
Źródło:
Wiadomości Parazytologiczne; 2009, 55, 4; 459-462
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Epidemiologiczne badania nad giardioza u mieszkancow Szczecina
Autorzy:
Ramisz, A
Krolikowska, A.
Balicka-Ramisz, A.
Powiązania:
https://bibliotekanauki.pl/articles/840500.pdf
Data publikacji:
1999
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Szczecin
choroby pasozytnicze
choroby czlowieka
Giardia intestinalis
pierwotniaki
parazytologia
giardioza
epidemiologia
mieszkancy
pasozyty czlowieka
Opis:
The aim of the studies was to establish the prevalence of Giardia intestinalis in Szczecin population. The studies were carried out in 1991-1996. In this period 46 584 persons - 22 371 adults and 24 213 children were examined. Faecal samples were examined with two methods - coprological and immunoenzymatic (ELISA) test. A total of 356 persons (0.78%) were found to be infected with G. intestinalis. No statistically significant differences between the infection rate of adults and children were observed. The adults were infected in 0.73% and the children in 0.80%. The analysis of the annual dynamics of G. intestinalis infection shows twice as large infection rate in September- October (1.18 %) as in the remaining months (0.66 %).
Źródło:
Annals of Parasitology; 1999, 45, 1; 69-74
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Epidemiologiczne badania nad giardiozą u mieszkańcow Szczecina
EPIDEMIOLOGICAL STUDIES ON GIARDIOSIS IN INHABITANTS OF SZCZECIN
Autorzy:
Ramisz, A.
Królikowska, A.
Balicka-Ramisz, A.
Powiązania:
https://bibliotekanauki.pl/articles/2148719.pdf
Data publikacji:
1999
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Szczecin
choroby pasozytnicze
choroby czlowieka
Giardia intestinalis
pierwotniaki
parazytologia
giardioza
epidemiologia
mieszkancy
pasozyty czlowieka
Opis:
The aim of the studies was to establish the prevalence of Giardia intestinalis in Szczecin population. The studies were carried out in 1991-1996. In this period 46 584 persons - 22 371 adults and 24 213 children were examined. Faecal samples were examined with two methods - coprological and immunoenzymatic (ELISA) test. A total of 356 persons (0.78%) were found to be infected with G. intestinalis. No statistically significant differences between the infection rate of adults and children were observed. The adults were infected in 0.73% and the children in 0.80%. The analysis of the annual dynamics of G. intestinalis infection shows twice as large infection rate in September- October (1.18 %) as in the remaining months (0.66 %).
Źródło:
Wiadomości Parazytologiczne; 1999, 45, 1; 69-74
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Occurrence of Cryptosporidium, Giardia and Toxoplasma in surface waters in the area of Cracow
Autorzy:
Polus, M.
Kocwa-Haluch, R.
Powiązania:
https://bibliotekanauki.pl/articles/207841.pdf
Data publikacji:
2014
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
protozoa
surface waters
Cryptosporidiosis
Cryptosporidium
Giardia intestinalis
oocysts
water samples
pierwotniaki
wody powierzchniowe
kryptosporydioza
oocysty
próbki wody
Opis:
Despite some knowledge on the risks from exposure to intestinal parasitic protozoa such as Cryptosporidium, Giardia and Toxoplasma, no occurrence studies of these protozoa were conducted in Cracow area. Therefore, the purpose of the work was to examine the presence of Cryptosporidium, Giardia and Toxoplasma in surface water samples including intakes and recreational waters. The study showed that the vast majority of water samples were positive for Cryptosporidium sp., and some of the samples were positive for Giardia intestinalis. None of the tested water samples showed the presence of Toxoplasma. Given the limitations of the research methods used and the limits of detection, these results indicated that the concentration of protozoan cysts/oocysts may range from <1 to dozens oocysts/cysts per liter of water. These concentrations are comparable to the values given in reports of major worldwide outbreaks of cryptosporidiosis or giardiasis, which indicating a need to further investigate Cracow's surface waters.
Źródło:
Environment Protection Engineering; 2014, 40, 2; 105-113
0324-8828
Pojawia się w:
Environment Protection Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Metabolizm serotoniny u dzieci zarażonych Giardia intestinalis
Autorzy:
Lucińska, A.
Prusek, W.
Galary, E.
Podwysocka, M.
Modrzycka, T.
Powiązania:
https://bibliotekanauki.pl/articles/2148530.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
choroby pasozytnicze
parazytologia lekarska
serotonina
Giardia intestinalis
dzieci
pierwotniaki
reakcje immunologiczne
metabolizm
giardioza
inwazja pasozytnicza
pasozyty czlowieka
Opis:
According to the data from the last 4 years, 3.8% of all diagnosed parasitoses are giardiases. During 5 years Giardia intestinalis was found in 24 chiidren hospitalized in the General Hospital of the Wrocław Voivodship: in 14 girls and 10 boys, 2-12 years old. The parasite was identified coproscopically and on the basis of clinical symptoms. Before the medication, the blood was taken from each child to determine the serotonin level and the (MAO) monoaminooxydaze, enzyme metabolizing serotonin acivity in the blood serum. The results were compared to the values obtained in the control group of 10 healthy children, 3-12 years old. III children were divided into two groups: first consisted of 12 children, 2-5 years old, second - of 12 children, 6-12 years old. In the first group 210% increase of the serotonin level in the blood and slight 32,4% increase of the MAO activity were observed in comparison with the control group. In the second group the serotonin level in the blood increased 221 % in comparison with the control, while the MAO actmty increased 45.1 %. In older children marced clinical symptoms could be observed, mainly malfunction of the alimentary tract, microelements shortage and anemia. The highest level of serotonin and MAO activity was found in the child with bile duet irritation. The reason for the increased level of serotonin and MAO activity can be pathogenic action of parasite's trophozoites and cysts on the jejunum mucosa and mobilization, proliferation and degranulation of tissue mastocytes. Serotonin, released from mastocytes, can be the cause of enhanced peristalsis and mucosa congestion.
Źródło:
Wiadomości Parazytologiczne; 2000, 46, 1; 149-155
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zwierzęta ziemnowodne jako źródło zanieczyszczenia wody Cryptosporidium i Giardia
Semi-aquatic animals as a source of water contamination with Cryptosporidium and Giardia
Autorzy:
Bajer, A.
Bednarska, M.
Paziewska, A.
Romanowski, J.
Siński, E.
Powiązania:
https://bibliotekanauki.pl/articles/2143808.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
pizmak
bobr europejski
zanieczyszczenia biologiczne
Giardia
pierwotniaki
rezerwuary pasozytow
pasozyty
zwierzeta ziemnowodne
zrodla zanieczyszczen
Cryptosporidium
zanieczyszczenia wod
Opis:
Cryptosporidium and Giardia spp. are parasitic protozoa localized in the alimentary tract of many animal species and humans. Each of these parasite species produces very resistant invasive forms (cysts and oocysts) excreted to the environment with feces of infected hosts. Water contaminated with cysts/oocysts constitutes one of the main transmission routes and is responsible for the majority of infections in humans. Cryptosporidium and Giardia spp. were found in many different species of animals, including livestock, pets and free living animals. The aim of our study was to determine the prevalence of these protozoa in selected species of semi−aquatic mammals and to estimate their role in water contamination. In years 1996–98 the prevalence of Cryptosporidium and Giardia infections was high in muskrats (Ondatra zibethicus) (58 and 87%, respectively). The origin of animals (farmed or free living) affected the prevalence of both parasites in European beavers (Castor fiber). The prevalence of infection increased in second period of study and was 4 and 19% for Cryptosporidium and 0 and 8% for Giardia spp. in the two studied periods, respectively. Both parasite species were also identified in water vole (Arvicola terrestris) and rat (Rattus norvegicus).
Źródło:
Wiadomości Parazytologiczne; 2008, 54, 4; 315-318
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zrodlo i znaczenie genetycznego zroznicowania populacji pasozytniczych pierwotniakow
Autorzy:
Majewska, A C
Sulima, P.
Powiązania:
https://bibliotekanauki.pl/articles/837049.pdf
Data publikacji:
1999
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
zroznicowanie genetyczne
Leishmania
Giardia
struktura populacji
pierwotniaki
parazytologia
zroznicowanie gatunkowe
Toxoplasma
populacje zwierzat
pasozyty
Trypanosoma
Cryptosporidium
Opis:
The application of biochemical and molecular techniques in parasitological studies has provided increasing evidences of genetic polymorphism among parasite populations. This review presents possible origins of genetic variation within populations of various protozoan species. Since the mode of reproduction has an important influence on genetic polymorphism within parasite populations these considerations refer mainly to some protozoan parasites which have various life cycles, e.g. Giardia, Trypanosoma, Cryptosporidium, Toxoplasma. Also other factors associated with parasites (such as: transmission and passage history in laboratory conditions; occurrence in different hosts or geographic regions; selective pressure of drugs; competitive interactions between populations) that affect parasite genetic diversity are discussed. However, the number of examined isolates of parasites and genetic markers, assortment of methods, probes, primers and reagents used is also of significance. The significance of genetic variability in parasite populations is still the subject of much interest and controversy. A simple interpretation of such variation is impossible because of the complexity of host-parasite interactions. The knowledge of parasite diversity at the nucleic acids level has continually increased, but a corect interpretation of this phenomenon requires at least the same knowledge of genetic variability in host populations. Nevertheless, genetic variability in protozoan parasites has many important implications, e.g. for taxonomy, epidemiology, control and evolution. Genetic differences within parasite populations might also be associated with phenotypic variability, e.g. virulence, antigenicity, infectivity, drug sensitivity, hostpreference etc.
Źródło:
Annals of Parasitology; 1999, 45, 3; 293-307
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Źródło i znaczenie genetycznego zróżnicowania populacji pasożytniczych pierwotniaków
SOURCE AND SIGNIFICANCE OF GENETIC POLYMORPHISM OF SELECTED PARASITIC PROTOZOA
Autorzy:
Majewska, A. C.
Sulima, P.
Powiązania:
https://bibliotekanauki.pl/articles/2148795.pdf
Data publikacji:
1999
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
zroznicowanie genetyczne
Leishmania
Giardia
struktura populacji
pierwotniaki
parazytologia
zroznicowanie gatunkowe
Toxoplasma
populacje zwierzat
pasozyty
Trypanosoma
Cryptosporidium
Opis:
The application of biochemical and molecular techniques in parasitological studies has provided increasing evidences of genetic polymorphism among parasite populations. This review presents possible origins of genetic variation within populations of various protozoan species. Since the mode of reproduction has an important influence on genetic polymorphism within parasite populations these considerations refer mainly to some protozoan parasites which have various life cycles, e.g. Giardia, Trypanosoma, Cryptosporidium, Toxoplasma. Also other factors associated with parasites (such as: transmission and passage history in laboratory conditions; occurrence in different hosts or geographic regions; selective pressure of drugs; competitive interactions between populations) that affect parasite genetic diversity are discussed. However, the number of examined isolates of parasites and genetic markers, assortment of methods, probes, primers and reagents used is also of significance. The significance of genetic variability in parasite populations is still the subject of much interest and controversy. A simple interpretation of such variation is impossible because of the complexity of host-parasite interactions. The knowledge of parasite diversity at the nucleic acids level has continually increased, but a corect interpretation of this phenomenon requires at least the same knowledge of genetic variability in host populations. Nevertheless, genetic variability in protozoan parasites has many important implications, e.g. for taxonomy, epidemiology, control and evolution. Genetic differences within parasite populations might also be associated with phenotypic variability, e.g. virulence, antigenicity, infectivity, drug sensitivity, hostpreference etc.
Źródło:
Wiadomości Parazytologiczne; 1999, 45, 3; 293-307
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optymalizacja metody wykrywania pierwotniaków pasożytniczych Cryptosporidium i Giardia w środowisku wodnym przy zastosowaniu automatycznej stacji płuczącej Filta-Max xpress
Optimization of Cryptosporidum and Giardia detection in water environment using automatic elution station Filta-Max xpress
Autorzy:
Matuszewska, R
Szczotko, M
Krogulska, B
Powiązania:
https://bibliotekanauki.pl/articles/872081.pdf
Data publikacji:
2012
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
srodowisko wodne
analiza wody
pierwotniaki
pasozyty
wykrywanie
Cryptosporidium
Giardia
uklady pluczace
automatyczna stacja pluczaca Filta-Max xpress
Opis:
Wprowadzenie. Obecność pierwotniaków pasożytniczych w wodzie przeznaczonej do spożycia jest przeważnie następstwem niewłaściwie prowadzonych procesów jej uzdatniania. Aktualnie, w Polsce, rutynowe badania wody w kierunku wykrywania pierwotniaków z rodzaju Cryptosporidium i Giardia nie są prowadzone. Cel badań. Celem badań była optymalizacja metody wykrywania oocyst Cryptosporidium sp. i cyst Giardia sp. w próbkach wody powierzchniowej w oparciu o założenia metodyczne zawarte w normie ISO 15553:2006 i przy zastosowaniu automatycznej stacji płuczącej Filta-Max xpress. Materiał i metoda. Badania wstępne prowadzono na próbkach wody kontaminowanej oocytami i cystami szczepów referencyjnych Cryptosporidium sp. i Giardia sp. Dalsze badania prowadzono z zastosowaniem próbek środowiskowych wody pobranej z kilku ujęć wody powierzchniowej (21 próbek z Wisły i 8 próbek z Zalewu Zegrzyńskiego). Próbki wody zagęszczano przy użyciu stacji Filta-Max xpress, a następnie oczyszczano je metodą separacji immunomagnetycznej. Cysty i oocysty barwiono za pomocą FITC i DAPI i prowadzono obserwacje mikroskopowe z wykorzystaniem mikroskopu epifluorescencyjnego. Wyniki. Zastosowanie 9-cyklowego systemu płuczącego w badaniach fortyfikowanych próbek wody, pozwoliło na uzyskanie średniego odzysku oocyst Cryptosporidium w wysokości 60,6% i cyst Giardia w wysokości 36,1%. W próbkach środowiskowych wody pochodzącej z ujęć powierzchniowych wykazano obecność poszukiwanych pierwotniaków. Cysty Giardia wykryto we wszystkich badanych próbkach wody powierzchniowej w liczbie od 1,0/10 l do 4,5/10 l pochodzącej z ujęcia na Zalewie Zegrzyńskim i z ujęcia na rzece Wiśle w liczbie od 1,0/10 l do 38,9/10 l. Oocysty Cryptosporidium obecne były w 50% próbek wody powierzchniowej z ujęć Zalewu Zegrzyńskiego i 47,6% próbek z Wisły, a ich liczba w obu przypadkach była zbliżona i wynosiła od 0,5 do 2,5 oocyst/10 l. Badania potwierdziły, że zastosowana metoda jest właściwa do wykrywania obecności pierwotniaków pasożytniczych w wodzie. Jej efektywność, szczególnie w przypadku próbek o dużej zawartości substancji organicznych i zawiesin, zależy od przygotowanych podpróbek i zmniejszenia szybkości płukania filtrów. Wnioski. Metoda wykrywania Cryptosporidium sp. i Giardia sp. z zastosowaniem automatycznej stacji płuczącej Filta- -Max xpress może być wykorzystywana w rutynowych badaniach oceny mikrobiologicznej jakości wody. Stwierdzanie obecności Cryptosporidium sp. i Giardia sp. w próbkach wody pochodzącej z ujęć wody powierzchniowej wskazuje na niebezpieczeństwo przedostawania się ich do wody przeznaczonej do spożycia, dlatego badania pierwotniaków pasożytniczych powinny być włączone do monitoringu wody.
Background. The presence of parasitic protozoa in drinking water is mostly a result of improperly maintened the water treatment process. Currently, in Poland the testing of Cryptosporidium and Giardia in water as a part of routine monitoring of water is not perform. Objective. The aim of this study was the optimization of the method of Cryptosporidium and Giardia detection in water according to the main principles of standard ISO 15553:2006 and using Filta-Max xpress automatic elution station. Material and method. Preliminary tests were performed on the samples contaminated with oocysts and cysts of reference strains of both parasitic protozoa. Further studies were carried out on environmental samples of surface water sampled directly from the intakes of water (21 samples from Vistula River and 8 samples from Zegrzynski Lake). Filtration process and samples volume reducing were performed using an automatic elution system Filta-Max xpress. Next, samples were purified during immunomagnetic separation process (IMS). Isolated cysts and oocysts were stained with FITC and DAPI and than the microscopic observation using an epifluorescence microscope was carried out. Results. Recovery of parasite protozoa in all contaminated water samples after 9-cycles elution process applied was mean 60.6% for Cryptosporidium oocysts and 36.1% for Giardia cysts. Studies on the environmental surface water samples showed the presence of both parasitic protozoa. Number of detected Giardia cysts ranged from 1.0/10 L up to 4.5/10 L in samples from Zegrzynski Lake and from 1.0/10 L up to 38.9 /10 L in samples from Vistula River. Cryptosporidium oocysts were present in 50% of samples from the Zegrzynski Lake and in 47.6% of samples from the Vistula River, and their number in both cases was similar and ranged from 0.5 up to 2.5 oocyst/10 L. The results show that applied procedure is appropriate for detection the presence of parasitic protosoan in water, but when water contains much amount of inorganic matter and suspended solids test method have to be modified like subsamples preparation and filtration process speed reduction. Conclusions. The applied method with the modification using Filta-Max xpress system can be useful for the routine monitoring of water. Detection of Cryptosporidium and Giardia in all samples of water taken from the intakes of surface water shows the possibility of transfering of the protozoan cysts into the water intended for the consumption, therefore the testing of Cryptosporidium and Giardia should be included into the monitoring of water.
Źródło:
Roczniki Państwowego Zakładu Higieny; 2012, 63, 4
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Metabolizm serotoniny u dzieci zarazonych Giardia intestinalis
Autorzy:
Lucinska, A
Prusek, W.
Galary, E.
Podwysocka, M.
Modrzycka, T.
Powiązania:
https://bibliotekanauki.pl/articles/839378.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
choroby pasozytnicze
parazytologia lekarska
serotonina
Giardia intestinalis
dzieci
pierwotniaki
reakcje immunologiczne
metabolizm
giardioza
inwazja pasozytnicza
pasozyty czlowieka
Opis:
According to the data from the last 4 years, 3.8% of all diagnosed parasitoses are giardiases. During 5 years Giardia intestinalis was found in 24 chiidren hospitalized in the General Hospital of the Wrocław Voivodship: in 14 girls and 10 boys, 2-12 years old. The parasite was identified coproscopically and on the basis of clinical symptoms. Before the medication, the blood was taken from each child to determine the serotonin level and the (MAO) monoaminooxydaze, enzyme metabolizing serotonin acivity in the blood serum. The results were compared to the values obtained in the control group of 10 healthy children, 3-12 years old. III children were divided into two groups: first consisted of 12 children, 2-5 years old, second - of 12 children, 6-12 years old. In the first group 210% increase of the serotonin level in the blood and slight 32,4% increase of the MAO activity were observed in comparison with the control group. In the second group the serotonin level in the blood increased 221 % in comparison with the control, while the MAO actmty increased 45.1 %. In older children marced clinical symptoms could be observed, mainly malfunction of the alimentary tract, microelements shortage and anemia. The highest level of serotonin and MAO activity was found in the child with bile duet irritation. The reason for the increased level of serotonin and MAO activity can be pathogenic action of parasite's trophozoites and cysts on the jejunum mucosa and mobilization, proliferation and degranulation of tissue mastocytes. Serotonin, released from mastocytes, can be the cause of enhanced peristalsis and mucosa congestion.
Źródło:
Annals of Parasitology; 2000, 46, 1; 149-155
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zwierzeta ziemnowodne jako zrodlo zanieczyszczenia wody Cryptosporidium i Giardia
Semi-aquatic animals as a source of water contamination with Cryptosporidium and Giardia
Autorzy:
Bajer, A.
Bednarska, M.
Paziewska, A.
Romanowski, J.
Sinski, E.
Powiązania:
https://bibliotekanauki.pl/articles/840330.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
pizmak
bobr europejski
zanieczyszczenia biologiczne
Giardia
pierwotniaki
rezerwuary pasozytow
pasozyty
zwierzeta ziemnowodne
zrodla zanieczyszczen
Cryptosporidium
zanieczyszczenia wod
Źródło:
Annals of Parasitology; 2008, 54, 4; 315-318
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Inwazje Giardia intestinalis u szynszyli małej (Chinchilla lanigera)
Parasitic infestation of Giardia intestinalis in long-tailed chinchilla (Chinchilla lanigera)
Autorzy:
Janczak, D.
Barszcz, K.
Cielecka, D.
Golab, E.
Powiązania:
https://bibliotekanauki.pl/articles/862038.pdf
Data publikacji:
2014
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
zwierzeta futerkowe
szynszyle
pasozyty zwierzat
pierwotniaki
Giardia intestinalis
cykl rozwojowy
inwazja pasozytnicza
diagnostyka
leczenie
profilaktyka
zwalczanie pasozytow zwierzat
Źródło:
Życie Weterynaryjne; 2014, 89, 10
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biologia, epidemiologia i diagnostyka chorobotworczych pierwotniakow przenoszonych za posrednictwem wody
Biology, epidemiology and diagnostics of pathogenic waterborne protozoan parasites
Autorzy:
Leonska-Duniec, A
Adamska, M.
Powiązania:
https://bibliotekanauki.pl/articles/838881.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
czynniki chorobotworcze
pasozyty jelitowe
pierwotniaki
Cryptosporidium
Giardia
Cyclospora cayetanensis
Isospora belli
Mikrosporidia
biologia
epidemiologia
diagnostyka
przenoszenie chorob
woda
zanieczyszczenia wod
zanieczyszczenia mikrobiologiczne
Źródło:
Annals of Parasitology; 2010, 56, 2; 125-132
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biologia, epidemiologia i diagnostyka chorobotwórczych pierwotniaków przenoszonych za pośrednictwem wody
Biology, epidemiology and diagnostics of pathogenic waterborne protozoan parasites
Autorzy:
Leońska-Duniec, A
Adamska M.
Powiązania:
https://bibliotekanauki.pl/articles/2143426.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
czynniki chorobotworcze
pasozyty jelitowe
pierwotniaki
Cryptosporidium
Giardia
Cyclospora cayetanensis
Isospora belli
Mikrosporidia
biologia
epidemiologia
diagnostyka
przenoszenie chorob
woda
zanieczyszczenia wod
zanieczyszczenia mikrobiologiczne
Opis:
Cryptosporidium, Giardia intestinalis, Cyclospora cayetanensis, Isosopra belli and micropsoridia are the most important and common pathogens found in humans and many other species of vertebrates. In humans, mainly in immunocompromised patients, children, pregnant women and elderly people, they are the most frequently identified protozoan parasites causing gastrointestinal disease worldwide. These pathogens have several transmission routes, including anthroponotic and zoonotic transmission. What is more, in many cases of epidemics caused by mentioned pathogens the major cause of infection was contaminated with these organisms water and food. In spite of many existing regulations of clearing and making use of drinking water supplies and recreational water, cosmopolitan protozoan parasites are still the danger of public health. These organisms are responsible for many waterborne outbreaks worldwide. Light microscopy and immunofluorescence assay have been used to identify these organisms in most laboratories. However, these traditional techniques have major limitations in the specific diagnosis, these methods are not sensitive enough to detect cysts or oocysts in environmental samples, so the new molecular tools must be applied. Recently, PCR-based techniques have been developed for detection and genetic characterization of the different species and population variants of protozoan parasites is central to the prevention, surveillance and control of gastrointestinal diseases. In this review were characterized biology, epidemiology and the progress in technology for detection and surveillance of the most important waterborne protozoan parasites.
Źródło:
Wiadomości Parazytologiczne; 2010, 56, 2; 125-132
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 16

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies