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Tytuł:
Protein kinases CKI and CKII are implicated in modification of ribosomal proteins of the yeast Trichosporon cutaneum
Autorzy:
Wojda, Iwona
Cytryńska, Małgorzata
Frajnt, Magdalena
Jakubowicz, Teresa
Powiązania:
https://bibliotekanauki.pl/articles/1043700.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
CKII
CKI
phosphorylation
ribosomal phosphoproteins
Trichosporon cutaneum
Opis:
Phosphorylation of acidic ribosomal proteins P1/P2-P0 is a common phenomenon in eukaryotic organisms. It was found previously that in Trichosporon cutaneum, unlike in other yeast species, in addition to the two acidic ribosomal proteins, two other proteins of 15 kDa and 19 kDa of the small ribosomal subunit were phosphorylated. Here we describe two protein kinases: CKI and CKII, which are engaged in the modification of T. cutaneum ribosomal proteins. The acidic ribosomal proteins and the protein of 19 kDa were modified by CKII associated with ribosomes, while the protein of 15 kDa was modified by CKI. Protein kinase CKI was purified from cell-free extract (CKIC) and from ribosomal fraction (CKIR). The molecular mass of CKIC was established at 33 kDa while that of CKIR at 35-37 kDa. A protein of 40 kDa copurified with CKIR but not CKIC. Heparin significantly increased 40 kDa protein phosphorylation level by CKIR. Microsequencing analysis revealed the presence of CKI recognition motifs in the N-terminal fragment of the 40 kDa protein.
Źródło:
Acta Biochimica Polonica; 2002, 49, 4; 947-957
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rabbit muscle fructose-1,6-bisphosphatase is phosphorylated in vivo.
Autorzy:
Rakus, Dariusz
Zarzycki, Marek
Dzugaj, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1043652.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
aldolase
muscle
phosphorylation
AMP
fructose-1,6-bisphosphatase
Opis:
Phosphorylated fructose-1,6-bisphosphatase (FBPase) was isolated from rabbit muscle in an SDS/PAGE homogeneous form. Its dephosphorylation with alkaline phosphatase revealed 2.8 moles of inorganic phosphate per mole of FBPase. The phosphorylated FBPase (P-FBPase) differs from the dephosphorylated enzyme in terms of its kinetic properties like Km and kcat, which are two times higher for the phosphorylated FBPase, and in the affinity for aldolase, which is three times lower for the dephosphorylated enzyme. ephosphorylated FBPase can be a substrate for protein kinase A and the amount of phosphate incorporated per FBPase monomer can reach 2-3 molecules. Since interaction of muscle aldolase with muscle FBPase results in desensitisation of the latter toward AMP inhibition (Rakus & Dzugaj, 2000, Biochem. Biophys. Res. Commun. 275, 611-616), phosphorylation may be considered as a way of muscle FBPase activity regulation.
Źródło:
Acta Biochimica Polonica; 2003, 50, 1; 115-121
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cloning and characterization of the gene encoding ribosomal P0 phosphoprotein from Neurospora crassa.
Autorzy:
Krokowski, Dawid
Tchórzewski, Marek
Grankowski, Nikodem
Powiązania:
https://bibliotekanauki.pl/articles/1043800.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
phosphorylation
cloning
ribosomal P0 protein
Neurospora crassa
Opis:
A gene for ribosomal protein P0 that belongs to the family of ribosomal P proteins was isolated from a Neurospora crassa cDNA library, using polyclonal antibodies against recombinant P0 protein from Saccharomyces cerevisiae. This is the first gene for ribosomal P0 protein to be cloned from filamentous fungi. The derived P0 protein sequence has a strong homology to other eukaryotic P0 proteins; yet, there is a notable alteration in the conservative C-terminal region, placing this protein among the unique sequences from protozoan parasites.
Źródło:
Acta Biochimica Polonica; 2002, 49, 1; 11-18
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Starches Modified by Combination of Phosphorylation and High-Voltage Electrical Discharge (HVED) Treatment
Autorzy:
Grgić, Ivanka
Grec, Marijana
Gryszkin, Artur
Zięba, Tomasz
Kopjar, Mirela
Ačkar, Đurđica
Jozinović, Antun
Miličević, Borislav
Zavadlav, Sandra
Babić, Jurislav
Powiązania:
https://bibliotekanauki.pl/articles/1363283.pdf
Data publikacji:
2021
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
cereal starch
tuber starch
HVED
phosphorylation
physicochemical properties
Opis:
Starch is extensively used in the food industry as a texture modifier, a fat substitute, and in other applications. To optimise starch functional properties for specific use, it is subjected to various modifications. High-voltage electrical discharge (HVED) treatment, as a non-thermal and rapid process, was applied in this research as a single method and in combination with phosphorylation in order to explore its potential for improving starch physicochemical properties. Maize, wheat, potato, and tapioca starches were modified, and Na5P3O10 and Na2HPO4 were used for phosphorylation. Starch gelatinisation parameters (by DSC); paste clarity; and contents of amylose, damaged starch, and resistant starch were determined; and FTIR-ATR spectra were recorded. All modifications reduced the enthalpy of gelatinisation and decreased contents of amylose, resistant starch, and damaged starch. The effect of the HVED treatment on starch properties depended on starch type and combinations with chemicals. HVED could act as an aid in the starch phosphorylation process since the properties analysed were more effectively improved when HVED was combined with phosphorylation than by phosphorylation alone.
Źródło:
Polish Journal of Food and Nutrition Sciences; 2021, 71, 1; 79-88
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A new approach to phosphorylation of nucleosides using oxyonium phosphobetaines as intermediates
Autorzy:
Materna, Magdalena
Stawinski, Jacek
Sobkowski, Michał
Powiązania:
https://bibliotekanauki.pl/articles/16673995.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
synthesis
phosphorylation
N-oxides
nucleotides
oxyonium phosphobetaines
H-phosphonates
Opis:
Oxyonium phosphobetaines are recently discovered molecules with a unique ¯O–P–O–N+ bond system, which makes them useful and versatile intermediates for the synthesis of phosphates and their derivatives. In this paper, the preliminary data on the application of these compounds in nucleoside phosphorylation were presented.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2023, 104, 1; 93-99
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
High sequence homology between protein tyrosine acid phosphatase from boar seminal vesicles and human prostatic acid phosphatase
Autorzy:
Wysocki, Paweł
Płucienniczak, Grażyna
Strzeżek, Jerzy
Powiązania:
https://bibliotekanauki.pl/articles/1040544.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
phosphorylation
boar
protein tyrosine phosphatase
seminal plasma
prostatic acid phosphatase
Opis:
Boar seminal vesicle protein tyrosine acid phosphatase (PTAP) and human prostatic acid phosphatase (PAP) show high affinity for protein phosphotyrosine residues. The physico-chemical and kinetic properties of the boar and human enzymes are different. The main objective of this study was to establish the nucleotide sequence of cDNA encoding boar PTAP and compare it with that of human PAP cDNA. Also, the amino-acid sequence of boar PTAP was compared with the sequence of human PAP. PTAP was isolated from boar seminal vesicle fluid and sequenced. cDNA to boar seminal vesicle RNA was synthesized, amplified by PCR, cloned in E. coli and sequenced. The obtained N-terminal amino-acid sequence of boar PTAP showed 92% identity with the N-terminal amino-acid sequence of human PAP. The determined sequence of a 354 bp nucleotide fragment (GenBank accession number: GQ184596) showed 90% identity with the corresponding sequence of human PAP. On the basis of this sequence a 118 amino acid fragment of boar PTAP was predicted. This fragment showed 89% identity with the corresponding fragment of human PAP and had a similar hydropathy profile. The compared sequences differ in terms of their isoelectric points and amino-acid composition. This may explain the differences in substrate specificity and inhibitor resistance of boar PTAP and human PAP.
Źródło:
Acta Biochimica Polonica; 2009, 56, 3; 481-486
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Short-term regulation of the mammalian pyruvate dehydrogenase complex
Autorzy:
Strumiło, Sławomir
Powiązania:
https://bibliotekanauki.pl/articles/1041314.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
pyruvate dehydrogenase complex
thiamine diphosphate
phosphorylation
dephosphorylation
regulation
allosteric effect
Opis:
In this minireview the main mechanism of control of mammalian pyruvate dehydrogenase complex (PDHC) activity by phosphorylation-dephosphorylation is presented in the first place. The information recently obtained in several laboratories includes new data about isoforms of the PDH converting enzymes (kinase and phosphatase) and their action in view of short-term regulation of PDHC. Moreover, interesting influence of exogenous thiamine diphosphate (TDP) and some divalent cations, especially Mn2+, on the kinetic parameters of PDHC saturated with endogenous tightly bound TDP, is discussed. This influence causes a shortening of the lag-phase of the catalyzed reaction and a strong decrease of the Km value of PDHC mainly for pyruvate. There are weighty arguments that the effects have an allosteric nature. Thus, besides reversible phosphorylation, also direct manifold increase of mammalian PDHC affinity for the substrate by cofactors seems an important aspect of its regulation.
Źródło:
Acta Biochimica Polonica; 2005, 52, 4; 759-764
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phosphorylation of basic amino acid residues in proteins: important but easily missed
Autorzy:
Cieśla, Joanna
Frączyk, Tomasz
Rode, Wojciech
Powiązania:
https://bibliotekanauki.pl/articles/1039902.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
basic amino acids
posttranslational modification
phosphorylation
acid-labile
base-stable
phosphoramidate
Opis:
Reversible phosphorylation is the most widespread posttranslational protein modification, playing regulatory role in almost every aspect of cell life. The majority of protein phosphorylation research has been focused on serine, threonine and tyrosine that form acid-stable phosphomonoesters. However, protein histidine, arginine and lysine residues also may undergo phosphorylation to yield acid-labile phosphoramidates, most often remaining undetected in conventional studies of protein phosphorylation. It has become increasingly evident that acid-labile protein phosphorylations play important roles in signal transduction and other regulatory processes. Beside acting as high-energy intermediates in the transfer of the phosphoryl group from donor to acceptor molecules, phosphohistidines have been found so far in histone H4, heterotrimeric G proteins, ion channel KCa3.1, annexin 1, P-selectin and myelin basic protein, as well as in recombinant thymidylate synthase expressed in bacterial cells. Phosphoarginines occur in histone H3, myelin basic protein and capsidic protein VP12 of granulosis virus, whereas phospholysine in histone H1. This overview of the current knowledge on phosphorylation of protein basic amino-acid residues takes into consideration its proved or possible roles in cell functioning. Specific requirements of studies on acid-labile protein phosphorylation are also indicated.
Źródło:
Acta Biochimica Polonica; 2011, 58, 2; 137-148
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Guard cells anion channel SLAC1 is activated by a set of kinases originating from three different families
Autorzy:
Diekmann, M.
Geiger, D.
Hedrich, R.
Powiązania:
https://bibliotekanauki.pl/articles/81003.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
drought stress
plant
guard cell
anion channel
abscisic acid
protein kinase
phosphorylation
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Functional and biochemical characterization of Arabidopsis calcium sensor SCS a potential regulator of SnRK2 protein kinases
Autorzy:
Bucholc, M.
Goch, G.
Ciesielski, A.
Anielska-Mazur, A.
Dobrowolska, G.
Powiązania:
https://bibliotekanauki.pl/articles/80164.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
biochemical characteristics
functional characteristics
Arabidopsis
protein kinase
water deficit
calcium binding protein
phosphorylation
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nuclear localization and binding affinity of STAT5b for the α2-macroglobulin gene promoter during rat liver development and the acute-phase response
Autorzy:
Mihailović, Mirjana
Dinić, Svetlana
Bogojević, Desanka
Ivanović-Matić, Svetlana
Uskoković, Aleksandra
Arambašić, Jelena
Grigorov, Ilijana
Grdović, Nevena
Vidaković, Melita
Martinović, Vesna
Petrović, Miodrag
Poznanović, Goran
Powiązania:
https://bibliotekanauki.pl/articles/1041082.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
rat liver development
phosphorylation
nuclear extract
nuclear matrix
STAT5b
α2-macroglobulin
Opis:
Expression of the rat α2-macroglobulin (MG) gene undergoes dynamic changes throughout an individual's life and during the acute-phase (AP) response. Details of the participation of the STAT family of transcription factors in its control remain incompletely understood. Here we examined the involvement of STAT5b in MG gene expression during development and the AP response. Immuno-blot analysis revealed the highest nuclear level of STAT5b in the fetus and during postnatal development, whereas in the adult it decreased. Stimulation of MG expression during the AP response was accompanied by a decrease in STAT5b. Examination of STAT5b localization revealed that the relative concentrations of STAT5b were higher in the nuclear matrix than in the nuclear extract. Affinity chromatography with the extended promoter region of the MG gene (-825/+12), followed by immuno-blot analysis, revealed dynamic changes in STAT5b binding. The highest concentration of the promoter-binding form of STAT5b was observed in the fetus. As postnatal development progressed, the level of promoter-bound STAT5b decreased and in the adult liver it was the lowest. Stimulation of MG gene expression during the AP response in both the fetus and adult was accompanied by significantly decreased STAT5b binding to the MG promoter. The AP response was accompanied by lower levels of STAT5b serine and tyrosine phosphorylation in both fetus and adult. In the nuclear matrix derived from adult tissues, tyrosine phosphorylated species were completely absent. We conclude that developmental-stage differences in the mechanisms that determine STAT5b nuclear localization contribute to its activity in vivo.
Źródło:
Acta Biochimica Polonica; 2007, 54, 2; 331-340
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Role of spruce respiratory burst oxidase homolog (RBOH) during lignin formation in Norway spruce
Autorzy:
Nickolov, K.
Laitinen, T.
Haggman, H.
Teeri, T.
Karkonen, A.
Powiązania:
https://bibliotekanauki.pl/articles/80939.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
Norway spruce
respiratory burst
lignin formation
hydrogen peroxide
NADPH oxidase
phosphorylation
protein-protein interaction
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. I. The technology for the preparation of the primers
Autorzy:
Borowicz, B P
Powiązania:
https://bibliotekanauki.pl/articles/65836.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
phosphorylation
plant protection
I-18 C gene
purification
Chironomus tentans
polymerase chain reaction
DNA fragment
oligonucleotide
Opis:
The above mentioned technology in a case of the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene is described i.e. the primers design, purification and phosphorylation of the oligonucleotide primers.
Dla określonego w tytule pracy, celu badań przedstawiono technologię przygotowywania starterów, w przypadku DNA odpowiadającego Otwartej Ramie Odczytu II genu I-18 C Chironomus tentans. Technologia ta obejmowała zaprojektowanie starterów dla PCR oraz oczyszczenie i fosforylację zsyntetyzowanych oligonukleotydowych starterów.
Źródło:
Journal of Plant Protection Research; 1997, 37, 1-2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
PP2A controls methionine metabolism elicited by intracellular H2O2
Autorzy:
Trotta, A.
Li, S.
Mhamdi, A.
Ravanel, S.
Noctor, G.
Kangasjarvi, S.
Powiązania:
https://bibliotekanauki.pl/articles/80912.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
intracellular signalling
plant cell
reactive oxygen species
protein
post-translational modification
phosphorylation
methionine
hydrogen peroxide
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Modyfikacje epigenetyczne jako potencjalne cele terapii antynowotworowych
Epigenetic modifications as potential targets of anti-cancer therapy
Autorzy:
Kulczycka, Anna
Bednarek, Ilona
Dzierżewicz, Zofia
Powiązania:
https://bibliotekanauki.pl/articles/1034844.pdf
Data publikacji:
2013
Wydawca:
Śląski Uniwersytet Medyczny w Katowicach
Tematy:
epigenetyka
dna
histony
metylacja
acetylacja
fosforylacja
terapie antynowotworowe
epigenetics
histones
methylation
acetylation
phosphorylation
anti-cancer treatments
Opis:
Epigenetics analyses inherited characteristics not directly connected to the DNA nucleotide sequence. It investigates the relationships between biochemical modifications and the expression of selected genes. Initially, it was thought that gene expression depends on information encoded in the DNA sequence. However, it was discovered that the activity of many enzymes like methylases, demethylases, acetylases, deacetylases is necessary to regulate this process and its dysregulations may lead to e.g. cancer initiation and progression. Epigenetics has an impact on neoplastic transformation by reducing the global level of DNA methylation and increasing the methylation level within tumour suppressor gene promoters, which significantly impairs the repression of carcinogenesis. Additionally, modifications of histone proteins, based on disorders of acetylation-deacetylation and methylation-demethylation processes, may lead to overexpression of genes involved in cancer development. Numerous examples have been described, among others breast, prostate and colon cancers, depending on the modification of histone amino tails, primarily of histone H3. For such reasons, the possibility of using many therapies which can reverse the negative effect of these modifications by e.g. DNA demethylation (DNA demethylating drugs) or re-acetylation of histone lysine resides (histone deacetylase inhibitors) is examined. In the near future, epigenetics probably will allow the effective treatment of some cancer diseases, although further research on the impact of enzymatic modifications on the development of carcinogenesis is still needed.
Epigenetyka zajmuje się badaniem cech dziedzicznych, które nie zależą bezpośrednio od sekwencji nukleotydowej w DNA, ale są rezultatem modyfikacji biochemicznych na ekspresję wybranych genów. Początkowo uważano, że ekspresja genów zależy tylko od informacji zapisanej zawartej w sekwencji DNA, z czasem okazało się, że liczne modyfikacje będące rezultatem działania różnych grup enzymów, w tym metylaz, demetylaz, acetylaz czy deacetylaz, wpływają na regulację tego procesu, a zaburzenia regulacji aktywności tych enzymów mogą prowadzić do wystąpienia i rozwoju m.in. nowotworów. Epigenetyczny aspekt rozwoju transformacji nowotworowej wskazuje na obniżenie globalnego poziomu metylacji DNA oraz podwyższenie poziomu metylacji w obrębie promotorów genów supresorowych, co znacząco upośledza represję nowotworzenia. Dodatkowo, modyfikacje białek histonowych, opierające się na dysregulacji procesów acetylacji–deacetylacji i metylacji – demetylacji, prowadzą do nadekspresji genów zaangażowanych w rozwój kancerogenezy. Opisane zostały liczne przykłady zależności wystąpienia nowotworów, m.in. raka sutka, stercza czy okrężnicy od wystąpienia danej modyfikacji reszt aminokwasowych białek histonowych, w tym głównie histonu H3. Z takich też przyczyn podejmowane są próby zastosowania terapii odwracających negatywny skutek wybranych modyfikacji, np. poprzez demetylację DNA (leki demetylujące DNA) czy reacetylację reszt lizynowych histonów (inhibitory decetylaz histonów). W niedalekiej przyszłości epigenetyka najprawdopodobniej u możliwi skuteczne leczenie części chorób nowotworowych, aczkolwiek konieczne są dalsze badania wpływu modyfikacji enzymatycznych na mechanizm rozwoju kancerogenezy.
Źródło:
Annales Academiae Medicae Silesiensis; 2013, 67, 3; 201-208
1734-025X
Pojawia się w:
Annales Academiae Medicae Silesiensis
Dostawca treści:
Biblioteka Nauki
Artykuł
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