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    Wyświetlanie 1-15 z 15
    Tytuł:
    Very High Concentration of D-Dimers in Portal Blood in Patients with Pancreatic Cancer
    Autorzy:
    Durczyński, Adam
    Szymański, Dariusz
    Nowicki, Michał
    Hogendorf, Piotr
    Poznańska, Grażyna
    Strzelczyk, Janusz
    Powiązania:
    https://bibliotekanauki.pl/articles/1396873.pdf
    Data publikacji:
    2012-12-01
    Wydawca:
    Index Copernicus International
    Tematy:
    D-dimers
    pancreatic cancer
    portal blood
    peripheral blood
    Opis:
    Nowadays, increasing attention has been focused on relation between increased D-dimer levels and cancer among patients without detectable thrombosis. The aim of the study was to measure plasma D-dimer levels in portal and peripheral blood in pancreatic cancer patients with absence of venous thromboembolism. Material and methods. Fifteen consecutive patients hospitalized in the Department of General and Transplant Surgery of Medical University in Łódź, from January to March 2012 who underwent surgery due to a pancreatic cancer were enrolled. At laparotomy, portal and peripheral blood were sampled concurrently. D-dimer and fibrinogen levels were measured. Moreover, to investigate overall coagulation function prothrombin time (PT), prothrombin index (PI), international normalized ratio (INR), thrombin time (TT), activated partial thromboplastin time (APTT), TT and APTT index were evaluated. Results. Peripheral plasma D-dimmer levels above normal range were found in 10/15 patients (66,67%), whereas D-dimer above normal values were confirmed in all portal blood samples. Mean D-dimer values were higher in portal than in peripheral blood (3279.37 vs 824.64, by 297%, p=0,025). These discrepancies were accompanied by normal limits of portal and peripheral levels of fibrinogen and comparable coagulation function indexes. Conclusion. Our preliminary study showed the close relation between activation of hemostasis, reflected by elevated D-dimers in portal blood and presence of pancreatic cancer. These data suggest that measurement of portal blood D-dimer levels may be a potentially useful technique for screening the pancreatic cancer.
    Źródło:
    Polish Journal of Surgery; 2012, 84, 10; 521-525
    0032-373X
    2299-2847
    Pojawia się w:
    Polish Journal of Surgery
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Dose- and time-dependent micronucleus induction in peripheral erythrocytes of catfish, Heteropneustes fossilis (Bloch) by zinc
    Autorzy:
    Talapatra, S.N.
    Banerjee, P.
    Mukhopadhyay, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/11433.pdf
    Data publikacji:
    2014
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    micronucleus
    peripheral blood
    erythrocyte
    catfish
    Heteropneustes fossilis
    zinc
    genotoxicity
    Opis:
    As far as the detection of metal genotoxicity in fish is concerned, micronucleus (MN) test is considered an extremely suitable measure. In this study, frequencies of micronucleated erythrocytes were scored in peripheral blood of catfish, Heteropneustes fossilis (bloch) after acute in-vivo exposure of zinc at different concentrations (5, 10 and 30 ppm) in the laboratory condition. These three concentrations of zinc were tested at different durations such as 24h, 48h, 72h and 96h respectively. Highly significant (P < 0.001) increased values were obtained for MN frequencies in the peripheral erythrocytes of exposed fishes compared to control groups of fishes. These results confirm that dose- and time-dependent micronucleation in the peripheral erythrocytes of fish after short-term exposure to zinc could provide valuable information regarding zinc containing effluent quality and also help in genetic biomonitoring with this test model. In this context safe concentration of zinc vis-a-vis genotoxicity range could be evaluated for future studies.
    Źródło:
    International Letters of Natural Sciences; 2014, 04
    2300-9675
    Pojawia się w:
    International Letters of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Investigations of the changes of peripheral blood T lymphocytes subpopulations using the flow cytofluorimetry technique in serum of T.spiralis infected mice
    Autorzy:
    Boczon, K.
    Wargin, B.
    Kostrzewa, A.
    Derda, M.
    Wiktorowicz, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/836212.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    peripheral blood
    mouse
    mice
    serum
    T lymphocyte
    trichinellosis
    blood
    larva
    cytofluorimetry technique
    Trichinella spiralis
    Źródło:
    Annals of Parasitology; 1998, 44, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Evaluation of DNA damage in white blood cells of healthy human volunteers using the alkaline comet assay and the chromosome aberration test
    Autorzy:
    Kopjar, Nevenka
    Želježić, Davor
    Garaj-Vrhovac, Verica
    Powiązania:
    https://bibliotekanauki.pl/articles/1041246.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    peripheral blood
    chromosome aberration test
    white blood cells
    DNA damage
    alkaline comet assay
    lymphocytes
    Opis:
    The present study was undertaken to contribute to the characterization of the degree of variability in baseline damage in white blood cells from control population, and to investigate how this variability is associated with external and internal factors. Altogether 170 healthy volunteers, randomly selected from the general population of the Republic of Croatia, participated in the study. Two sensitive tests: the alkaline comet assay and the chromosome aberration test were applied to study the background levels of DNA damage in their white blood cells. The results point to inter-individual differences, indicating different genome sensitivity. As revealed by both assays, the background levels of DNA damage were mostly influenced by smoking habit as well as medical exposure (especially to diagnostic X-rays). Sex and age of subjects did not significantly influence the values of DNA damage recorded in the white blood cells. Although higher levels of DNA damage were recorded in blood samples collected during winter and autumn, they were mostly influenced by medicinal exposure and smoking habit. Statistical evaluation of the data confirmed that a positive correlation exists between DNA migration and the number of long-tailed nuclei found with the comet assay and the total number of chromosome aberrations. The data obtained can serve as control values in forthcoming biomonitoring studies.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 2; 321-336
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of glucocorticoid [depemedrol] treatment on the changes of peripheral blood T lymphocytes in serum of T.spiralis infected mice
    Autorzy:
    Boczon, K.
    Kostrzewa, A.
    Wargin, B.
    Derda, M.
    Wiktorowicz, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/840872.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    glucocorticoid
    parasite
    peripheral blood
    infection
    mouse
    mice
    serum
    T lymphocyte
    trichinellosis
    depomedrol
    blood
    Trichinella spiralis
    Źródło:
    Annals of Parasitology; 1998, 44, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Reduced expression of AURKA in peripheral blood of breast cancer patients
    Autorzy:
    Goh, L.P.W.
    See, E.U.H.
    Chua, K.H.
    Lee, P.-C.
    Powiązania:
    https://bibliotekanauki.pl/articles/81279.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    breast cancer
    patient
    Aurora kinase
    gene expression
    peripheral blood
    cell cycle
    quantitative real-time polymerase chain reaction
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2018, 99, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Contact allergy to nickel: Patch test score correlates with IL-5, but not with IFN-gamma nickel-specific secretion by peripheral blood lymphocytes
    Autorzy:
    Czarnobilska, E
    Jenner, B.
    Kaszuba-Zwoinska, J.
    Kapusta, M.
    Obtulowicz, K.
    Thor, P.
    Spiewak, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/49735.pdf
    Data publikacji:
    2009
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    allergic contact dermatitis
    patch test
    contact allergy
    lymphocyte
    interleukin 5
    interferon gamma
    risk estimation
    nickel
    peripheral blood
    chronic disease
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2009, 16, 1; 37-41
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of Biolex Beta-HP on phagocytic activity and oxidative metabolism of peripherial blood granulocytes and monocytes in rats intoxicated by cyclophosphamide
    Autorzy:
    Wojcik, R
    Powiązania:
    https://bibliotekanauki.pl/articles/32265.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    beta-1,3-1,6-D-glucan
    cyclophosphamide
    rat
    phagocytic activity
    oxidative metabolism
    granulocyte
    monocyte
    flow cytometry
    peripheral blood
    immunosuppression
    blood neutrophil
    Opis:
    The objective of this study was to determine the effect of ß-l,3/l,6-D-glucan (Biolex-Beta HP) on the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in rats intoxicated by cyclophosphamide. The experimental material comprised 40 adult Wistar rats aged 14 weeks, divided into two equal groups, a control group and an experimental group, each of 20 adult rats, including 10 males and 10 females. In the course of 3 successive days, 20 rats from the experimental group were administered cyclophosphamide intramuscularly at a dose of 50 mg/kg body weight/day. On the 8th day of the experiment, 10 control group (K) rats and 10 experimental group (C) rats were sacrificed. Arterial blood samples were collected and diluted with heparin to determine and compare the phagocytic activity (Phatogest) and oxidative metabolism (Bursttest) of peripheral blood granulocytes and monocytes by flow cytometry. Starting on the 8lh day of the experiment, the feed of the remaining 10 rats from the experimental group (C+G) and 10 rats from the control group (G) was supplemented with Biolex-Beta HP at a dose of 50 mg/kg body weight/day for 14 consecutive days. On day 22, arterial blood samples were collected from all (C+G) and (C) group rats, diluted with heparin to determine and compare the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes by flow cytometry. The results showed that Biolex-Beta HP modulated the phagocytic activity and oxidative metabolism of blood neutrophils and monocytes suppressed by cyclophosphamide in rats. The immunorestoring activity of Biolex-Beta HP was observed in this study.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 1; 181-188
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cellular immune response of pigeons in the conditions of endotoxin fever and pyrogenic tolerance
    Autorzy:
    Dudek, K.
    Bednarek, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/30231.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    cellular immune response
    endotoxin fever
    fever
    pigeon
    pyrogenic tolerance
    bird
    Escherichia coli
    defense mechanism
    bacteria
    Salmonella
    Pseudomonas
    peripheral blood
    immunophenotyping
    Opis:
    The aim of this study was to investigate changes in selected parameters of cellular immune response in the conditions of endotoxin fever and pyrogenic tolerance in pigeons. On the first day of observation the experimental birds (n=18) were intravenously injected with Escherichia coli LPS at a dose of 10 μg/kg b.w., while the control animals (n=6) received apyrogenic physiological saline also in the form of injection. On the second and the third day of the experiment LPS was injected additionally at 24 h intervals. Four and a half hours after the saline and pyrogen administration blood samples were collected from the control and experimental pigeons. The following immunological assays were performed: WBC, leucogram and immunophenotyping of lymphocyte subsets in peripheral blood, i.e. CD 3+ (T lymphocytes), CD 4+ (T helper lymphocytes) and CD 8+ (T suppressor/ cytotoxic lymphocytes) cells. In the conditions of endotoxin fever (i.e. after the first LPS injection) leucopenia, monocytopenia, heterophilia and eosinophilia were observed. Additionally, the immunophenotyping of peripheral blood lymphocytes indicated an increase in percentage of CD 3+, CD 4+ and CD 8+ cells in response to the single injection of LPS. In contrast, the consecutive injections of LPS, which created a pyrogenic tolerance effect, caused a decrease in WBC value, heteropenia, eosinopenia and lymphocytosis. Moreover, during this state an increase in percentage of CD 3+ and CD 8+ cells was demonstrated in contrast to the percentage of CD 4+ lymphocytes. The general tendencies in cellular immune response of the affected pigeons in the conditions of endotoxin fever and pyrogenic tolerance aim at activation of defence mechanisms against LPS for its prompt elimination from the animal’s organism.
    Źródło:
    Polish Journal of Veterinary Sciences; 2011, 14, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Evaluation of the influence of meloxicam and flunixin meglumine on the apoptosis of peripheral blood CD4plus and CD8plus T cells in calves
    Autorzy:
    Maslanka, T
    Jaroszewski, J.J.
    Markiewicz, W.
    Jabubowski, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/31556.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    peripheral blood
    T cell
    calf
    CD4plus T cell
    CD8plus T cell
    apoptosis
    meloxicam
    flunixin meglumine
    lymphocyte
    antiinflammatory drug
    Opis:
    The aim of the study was to determine whether treatment with recommended doses of meloxicam or flunixin had an effect on the apoptosis of peripheral blood T lymphocytes in calves. The study was carried out on 4-5 months old calves (n = 24, 8 per group). Experimental animals were injected subcutaneously with a single dose of 0.5 mg . kg-1 of meloxicam or intravenously with 3 doses of 2.2 mg . kg-1 day-1 of flunixin. The non-treatment animals served as control. Blood samples were taken at day 0 and at days 1, 2, 3, 5, 7 and 14 after the first NSAIDs injection. Apoptosis was determined by flow cytometry using Annexin V-PE/7-AAD staining. The kinetic analysis of apoptosis in the total lymphocyte population, as well as in the CD4+ and CD8+ subsets did not reveal significant differences in the frequency of early apoptotic cells between control and experimental groups throughout the period studied. Although, 24 h after administration of the first dose of NSAIDs, late-stage apoptosis/necrosis was significantly increased in the total lymphocyte population (the meloxicam group), as well as in the CD4+ (the meloxicam group and the flunixin group) and CD8+ (the flunixin group) subsets of T cells. However, this disturbance was transient, relatively poorly expressed and, thus, unlikely to be of clinical significance. Our results indicate that the use of meloxicam or flunixin in accordance with the recommended dosage regimen in cattle do not have a clinically significant influence on apoptosis of peripheral blood T cells.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 1; 3-12
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Response of olfactory receptors and blood cells of Lake Baikal fish to phenol exposure
    Autorzy:
    Yakhnenko, V.
    Klimenkov, I.
    Pastukhova, N.
    Powiązania:
    https://bibliotekanauki.pl/articles/363284.pdf
    Data publikacji:
    2010
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    komórka
    ryby endemiczne
    system immunologiczny
    ryby nieendemiczne
    nabłonek węchowy
    krew obwodowa
    cells
    endemic fish
    immune system
    nonendemic fish
    olfactory epithelium
    peripheral blood
    Opis:
    Exposure to phenol causes ultrastructural disorders in supporting and receptor cells in the peripheral segment of the olfactory organ of yellowfin (Cottocomephorus grewingkii) and in cells in peripheral blood of stone sculpins (Paracottus knerii), yellowfin and perch (Perca fluviatilis). Identical responses to phenol exposure in the blood cells were observed in endemic Lake Baikal fish and in nonendemic fish. Differences were found only in the rate and intensity of the recovery response. Fast activation of immune processes were found in the coastal nonendemic perch, but they were slow in the stone sculpin, a coastal endemic species.
    Źródło:
    Environmental Biotechnology; 2010, 6, 2; 66-73
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The stimulation and inhibition of T cell proliferation by Helicobacter pylori components
    Autorzy:
    Chmiela, M
    Lelwala-Guruge, J.A.
    Wadstrom, T.
    Rudnicka, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/69409.pdf
    Data publikacji:
    1996
    Wydawca:
    Polskie Towarzystwo Fizjologiczne
    Tematy:
    polyacrylamide gel electrophoresis
    blood leucocyte
    sodium dodecyl sulphate
    horse
    stimulation
    macrophage
    chronic active gastritis
    Helicobacter pylori
    antibody
    gastric carcinoma
    cell proliferation
    peripheral blood
    ulcer
    plasma cell
    lymphocyte
    infiltration
    inhibition
    gastric lymphoma
    Źródło:
    Journal of Physiology and Pharmacology; 1996, 47, 1
    0867-5910
    Pojawia się w:
    Journal of Physiology and Pharmacology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ocena czestosci wystepowania mikrojader w erytrocytach myszy eksponowanych subchronicznie na promieniowanie jonizujace i nonylofenol
    Frequency of micronuclei in erythrocytes of mice subchronic exposed to ionising radiation and nonylphenol
    Autorzy:
    Dobrzynska, M M
    Powiązania:
    https://bibliotekanauki.pl/articles/871848.pdf
    Data publikacji:
    2008
    Wydawca:
    Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
    Tematy:
    zwierzeta laboratoryjne
    myszy
    promieniowanie jonizujace
    nonylofenol
    krew obwodowa
    szpik kostny
    mikrojadra
    retikulocyty
    erytrocyty polichromatyczne
    laboratory animal
    mouse
    ionizing radiation
    nonylphenol
    peripheral blood
    bone marrow
    micronucleus
    reticulocyte
    polychromatic erythrocyte
    Opis:
    Celem pracy było określenie indukcji mikrojąder w retikulocytach i erytrocytach polichromatycznych myszy pod wpływem subchronicznej ekspozycji na promieniowanie X, nonylofenol (NF) lub na skojarzone działanie obu czynników. Myszy Pzh: SFIS narażane były przez 8 tygodni, 5 razy w tygodniu. Dawki wynosiły 0.05 Gy lub 0,10 Gy promieniowania X, 25 mg/kg mc lub 50 mg/kg mc nonylofenolu, a w przypadku skojarzonego działania 0,05 Gy + 25 mg/kg mc NF lub 0,10 Gy + 50 mg/kg mc NF. Zarówno promieniowania X, jak i NF działając oddzielnie indukowały powstawanie mikrojąder w retikulocytach i erytrocytach polichromatycznych myszy. Skojarzone działanie obu czynników w retikulocytach krwi obwodowej oraz w erytrocytach polichromatycznych szpiku kostnego indukowało zwiększoną częstość występowania mikrojąder w porównaniu do efektów działania każdego z czynników oddzielnie. W retikulocytach szpiku kostnego skojarzone działanie obu czynników w małych dawkach (0,05 Gy + 50 mg/kg mc NF) powodowało zwiększenie efektu mutagennego. Natomiast, w przypadku skojarzenia obu czynników w większych dawkach (0,10 Gy + 50 mg/kg mc NF), nonylofenol może wywierać efekt ochronny w stosunku do DNA retikulocytów powodując zmniejszenie uszkodzeń indukowanych przez promieniowanie X.
    The aim of the research was to investigate the level of micronuclei induction in reticulocytes and polychromatic erythrocytes of mice following subchronic exposure to X-rays, nonylphenol (NP) or to a combination of both. Male mice Pzh:SFIS were exposed during 8 weeks, 5 day per week to doses 0.05 Gy and 0.10 Gy of X-rays, 25 mg/kg mc and 50 mg/kg bw of nonylphenol as well as to 0.05 Gy + 25 mg/kg bw NP and 0.10 Gy + 50 mg/kg bw NP for combined exposure. Both X-rays and NP, acting alone induced micronuclei in reticulocytes and in polychromatic erythrocytes of mice. Combined X-rays-NP exposure of peripheral blood reticulocytes and bone marrow polychromatic erythrocytes caused enhanced frequency of micronuclei compared to the effect of each agent alone. In bone marrow reticulocytes, combined exposure in lower doses (0.05 Gy + 25 mg/kg bw NP) induced enhanced mutagenic effect. Contrary, after combined exposure to both agents in higher doses (0.10 Gy + 50 mg/kg bw NP), nonylphenol may protected DNA of reticulocytes against damage induced by X-rays.
    Źródło:
    Roczniki Państwowego Zakładu Higieny; 2008, 59, 3; 309-318
    0035-7715
    Pojawia się w:
    Roczniki Państwowego Zakładu Higieny
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Źródła komórek macierzystych krwiotworzenia dla potrzeb transplantacji
    Sources of hematopoietic stem cells for transplantation
    Autorzy:
    Rzepecki, Piotr
    Gawroński, Krzysztof
    Młot, Beata
    Oborska, Sylwia
    Pielichowski, Wojciech
    Waśko-Grabowska, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1031036.pdf
    Data publikacji:
    2011
    Wydawca:
    Medical Communications
    Tematy:
    bone marrow
    growth factors
    hematopoietic stem cells transplantation
    mobilization of hematopoietic stem cells
    peripheral blood
    umbilical cord blood
    czynniki wzrostu
    krew obwodowa
    krew pępowinowa
    mobilizacja komórek macierzystych krwiotworzenia
    przeszczepianie krwiotwórczych komórek macierzystych
    szpik kostny
    Opis:
    Bone marrow transplantation dates back to the ‘50s, but greatest progress in this therapeutic modality applied successfully in the treatment of several conditions took place mostly during the past 25 years. Among all organ transplantations, bone marrow transplants are second only to kidney transplants. In Poland this therapeutic technique also undergoes a rapid development. During the past 10 years, absolute numbers of various forms of transplantation increased 100-fold, starting from 6 procedures in 1989 to over 700 at present. Essentially, the term bone marrow transplantation (BMT), present in names of international associations and registers, is in fact a colloquialism (e.g. European Group for Blood and Marrow Transplantation). A much more appropriate term would be hematopoietic cell transplantation (HCT). This is because progenitor cells for transplantation may be obtained not only from bone marrow, but also from peripheral blood and umbilical cord blood. The term hematopoietic cell transplantation has a much broader meaning and includes: classic transplantation of bone marrow obtained at surgery, transplantation of peripheral blood stem cells (PBSCT) and umbilical cord blood-derived stem cells. All hematopoietic stem cells express on their surface the CD34+ antigen and glycosylated transmembrane protein, a member of the family of adhesion molecules. In healthy persons, expression of this antigen in bone marrow cells is at the level of 1-3%, while in peripheral blood – 0.01-0.1%, and in umbilical cord blood – 0.1-0.4%. The first source (transplantation material) of hematopoietic progenitor cells was bone marrow, while transplantation of stem cells obtained from peripheral blood and umbilical cord blood started much later (during the ‘80s of the past century).
    Źródło:
    Current Gynecologic Oncology; 2011, 9, 3; 186-198
    2451-0750
    Pojawia się w:
    Current Gynecologic Oncology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Indukcja mikrojąder w krwi obwodowej i szpiku kostnym samców myszy narażanych subchronicznie na działanie promieniowania X i bisfenolu A
    Induction of micronuclei in peripheral blood and bone marrow reticulocytes of male mice after subchronic exposure to X-rays and bisphenol A
    Autorzy:
    Radzikowska, J.
    Gajowik, A.
    Dobrzynska, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/873526.pdf
    Data publikacji:
    2012
    Wydawca:
    Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
    Tematy:
    bisfenol A
    promieniowanie jonizujace
    dzialanie skojarzone
    mikrojadra
    krew obwodowa
    szpik kostny
    retikulocyty
    myszy
    samce
    zwierzeta laboratoryjne
    bisphenol A
    ionizing radiation
    combined action
    micronucleus
    peripheral blood
    bone marrow
    reticulocyte
    mouse
    male
    laboratory animal
    Opis:
    Wprowadzenie.Promieniowanie jonizujące i ksenoestrogeny występują powszechnie w środowisku człowieka. Bisfenol A (BPA) używany jest podczas produkcji poliwęglanów oraz żywic epoksydowych, stanowiących składnik m.in. soczewek do okularów, wypełnień dentystycznych, płyt CD, szyb okiennych, pokryw instrumentów, opakowań oraz pojemników na napoje, ale także wyrobów dla dzieci, butelek, talerzyków, kubeczków oraz elementów smoczków. Żywice epoksydowe wchodzą też w skład powłok wewnętrznych pojemników do przechowywania żywności. Promieniowanie jonizujące wykorzystywane jest m.in. w diagnostyce rentgenowskiej, terapii chorób nowotworowych, w przemyśle, nauce. Cel badań.Celem badań było określenie wpływu bisfenolu A, promieniowania X oraz skojarzonego działania obu czynników na indukcję mikrojąder w retikulocytach krwi obwodowej i szpiku kostnego myszy laboratoryjnych. Materiał i metoda.Doświadczenie prowadzono na samcach myszy Pzh: Sfis przez 8 tygodni. Zwierzętom podawano bisfenol A w wodzie do picia (5 mg/kg mc, 10 mg/kg mc, 20 mg/kg mc), napromieniano dawką 0,05 Gy promieniowania X albo poddawano skojarzonemu działaniu obu tych czynników (0,05 Gy + 5 mg/kg mc BPA). Krew z żyły ogonowej pobierano po upływie 1, 4 i 8 tygodni od rozpoczęcia ekspozycji, a szpik kostny tylko po zakończeniu narażania. Oceniano częstość występowania mikrojąder w retikulocytach. Wyniki. Zarówno bisfenol A jak i promieniowanie jonizujące stymulowały indukcję mikrojąder w retikulocytach krwi obwodowej i szpiku kostnego. W następstwie napromieniania zwierząt promieniowaniem X indukcja mikrojąder wzrastała, podczas gdy w rezultacie podawania bisfenolu A malała proporcjonalnie do czasu trwania ekspozycji.. Skojarzone działanie promieniowania jonizującego i BPA indukowało występowanie mikrojąder ze znacznie wyższą częstością w porównaniu do efektów działania samego BPA. Częstość występowania mikrojąder w krwi obwodowej zwiększała się w miarę upływu czasu od rozpoczęcia doświadczenia. W szpiku kostnym we wszystkich grupach obserwowano znacznie niższą liczebność retikulocytów z mikrojądrami niż w krwi obwodowej. Wnioski. Subchroniczne narażenie na bisfenol A prowadzi do zmniejszenia wrażliwości materiału genetycznego retikulocytów na indukcję uszkodzeń. Promieniowanie X jest prawdopodobnie czynnikiem decydującym o uszkodzeniu DNA w następstwie skojarzonego działania.
    Background. Ionizing radiation and xenoestrogens are widely present in the human environment. Bisphenol A (BPA) is used to manufacture polycarbonate plastics, epoxy and polyester resins. BPA is present in a great variety of products including: baby bottles, compact disks, thermal paper, safety helmets, bullet resistant laminate, plastic windows, car parts, adhesives, protective coatings, powder paints, polycarbonate bottles and containers, the sheathing of electrical and electronic parts, dental fillings. Food and beverage cans are protected from rusting and corrosion by the application of epoxy resins as inner coatings. Human activities involving the use of radiation and radioactive materials in industry, agriculture and research cause radiation exposure in addition to natural exposure coming from cosmic rays and naturally occurring radioactive substances. Objective. The aim of the study was to estimate the effects of bisphenol A, X-rays and combined exposure to X-rays and bisphenol A on the induction of micronuclei in the peripheral blood and in bone marrow reticulocytes of laboratory mice. Material and method. Pzh-Sfis male mice were exposed for 8 weeks. Animals were treated with bisphenol A diluted in drinking water (5 mg/kg bw, 10 mg/kg bw, 20 mg/kg bw), irradiated 0.05 Gy of X-rays or exposed to a combination of both (0.05 Gy + 5 mg/kg bw BPA). The samples of peripheral blood were taken at 1, 4 and 8 week following the start of exposure, whereas the bone marrow after the end of experiment, only. The induction of micronuclei in reticulocytes were evaluated by using fluorescence microscope. Results. Bisphenol A as well as ionizing radiation stimulated induction of micronuclei in peripheral blood and bone marrow reticulocytes. After the irradiation the level of micronuclei increased, whereas after exposure to BPA decreased related to time expired from beginning of experiment. Combined exposure of ionizing radiation and bisphenol A induced significantly higher frequency of micronuclei compared to the effect produced by BPA alone. The frequency of micronuclei in peripheral blood reticulocytes increased during the experiment. In all groups, the significantly lower induction of micronuclei in reticulocytes of bone marrow than of peripheral blood were observed. The levels of micronuclei in mice exposed to a combination of X-rays and BPA or to irradiation alone were slightly higher compared to those administered to BPA alone. Conclusions. Bisphenol A induced micronuclei in peripheral blood and bone marrow reticulocytes. Subchronic BPA exposure leads to diminished sensitivity of genetic material of reticulocytes on the induction of damage. X-rays is probably the agent which decided about DNA damage following combined exposure.
    Źródło:
    Roczniki Państwowego Zakładu Higieny; 2012, 63, 1
    0035-7715
    Pojawia się w:
    Roczniki Państwowego Zakładu Higieny
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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