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    Wyświetlanie 1-4 z 4
    Tytuł:
    Zrodlo i znaczenie genetycznego zroznicowania populacji pasozytniczych pierwotniakow
    Autorzy:
    Majewska, A C
    Sulima, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/837049.pdf
    Data publikacji:
    1999
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    zroznicowanie genetyczne
    Leishmania
    Giardia
    struktura populacji
    pierwotniaki
    parazytologia
    zroznicowanie gatunkowe
    Toxoplasma
    populacje zwierzat
    pasozyty
    Trypanosoma
    Cryptosporidium
    Opis:
    The application of biochemical and molecular techniques in parasitological studies has provided increasing evidences of genetic polymorphism among parasite populations. This review presents possible origins of genetic variation within populations of various protozoan species. Since the mode of reproduction has an important influence on genetic polymorphism within parasite populations these considerations refer mainly to some protozoan parasites which have various life cycles, e.g. Giardia, Trypanosoma, Cryptosporidium, Toxoplasma. Also other factors associated with parasites (such as: transmission and passage history in laboratory conditions; occurrence in different hosts or geographic regions; selective pressure of drugs; competitive interactions between populations) that affect parasite genetic diversity are discussed. However, the number of examined isolates of parasites and genetic markers, assortment of methods, probes, primers and reagents used is also of significance. The significance of genetic variability in parasite populations is still the subject of much interest and controversy. A simple interpretation of such variation is impossible because of the complexity of host-parasite interactions. The knowledge of parasite diversity at the nucleic acids level has continually increased, but a corect interpretation of this phenomenon requires at least the same knowledge of genetic variability in host populations. Nevertheless, genetic variability in protozoan parasites has many important implications, e.g. for taxonomy, epidemiology, control and evolution. Genetic differences within parasite populations might also be associated with phenotypic variability, e.g. virulence, antigenicity, infectivity, drug sensitivity, hostpreference etc.
    Źródło:
    Annals of Parasitology; 1999, 45, 3; 293-307
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Źródło i znaczenie genetycznego zróżnicowania populacji pasożytniczych pierwotniaków
    SOURCE AND SIGNIFICANCE OF GENETIC POLYMORPHISM OF SELECTED PARASITIC PROTOZOA
    Autorzy:
    Majewska, A. C.
    Sulima, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/2148795.pdf
    Data publikacji:
    1999
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    zroznicowanie genetyczne
    Leishmania
    Giardia
    struktura populacji
    pierwotniaki
    parazytologia
    zroznicowanie gatunkowe
    Toxoplasma
    populacje zwierzat
    pasozyty
    Trypanosoma
    Cryptosporidium
    Opis:
    The application of biochemical and molecular techniques in parasitological studies has provided increasing evidences of genetic polymorphism among parasite populations. This review presents possible origins of genetic variation within populations of various protozoan species. Since the mode of reproduction has an important influence on genetic polymorphism within parasite populations these considerations refer mainly to some protozoan parasites which have various life cycles, e.g. Giardia, Trypanosoma, Cryptosporidium, Toxoplasma. Also other factors associated with parasites (such as: transmission and passage history in laboratory conditions; occurrence in different hosts or geographic regions; selective pressure of drugs; competitive interactions between populations) that affect parasite genetic diversity are discussed. However, the number of examined isolates of parasites and genetic markers, assortment of methods, probes, primers and reagents used is also of significance. The significance of genetic variability in parasite populations is still the subject of much interest and controversy. A simple interpretation of such variation is impossible because of the complexity of host-parasite interactions. The knowledge of parasite diversity at the nucleic acids level has continually increased, but a corect interpretation of this phenomenon requires at least the same knowledge of genetic variability in host populations. Nevertheless, genetic variability in protozoan parasites has many important implications, e.g. for taxonomy, epidemiology, control and evolution. Genetic differences within parasite populations might also be associated with phenotypic variability, e.g. virulence, antigenicity, infectivity, drug sensitivity, hostpreference etc.
    Źródło:
    Wiadomości Parazytologiczne; 1999, 45, 3; 293-307
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Metody roznicowania gatunkow nicieni z rodzaju Trichinella
    Methods and tools for parasite differentiation within the genus Trichinella
    Autorzy:
    Pastusiak, K
    Powiązania:
    https://bibliotekanauki.pl/articles/840918.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    izoenzymy
    hybrydyzacja kwasow nukleinowych
    roznicowanie gatunkow
    pasozyty
    nicienie
    sekwencje DNA
    Warszawa konferencja
    DNA mitochondrialny
    parazytologia
    konferencje
    Trichinella
    zroznicowanie gatunkowe
    lancuchowa reakcja polimerazy
    biologia molekularna
    znakowanie DNA
    Opis:
    This review summarizes the major biological, biochemical and molecular methods which have been developed during last 20 years to distinguish parasites of the genus Trichinella. From the time of the discovery of Trichinella in 1835 until the 1970, it was assumed that trichinellosis was caused by a single species of parasite, Trichinella spiralis. Many biological parameters have been compared to differentiate the parasite, such as host specificity, geographical distribution, reproductive abilities, nurse cell development and resistance to freezing. Now, investigators realize that the genus Trichinella is a much more complex group of parasites and simple biological methods are unsufficient. In order to identify and better characterize the species and genotypes of Trichinella it was necessary to develop more sensitive techniques. First, for detecting Trichinella infection immunological methods have been used, such as detection of antibodies in host blood and antigens of parasites using monoclonal antibodies against immunodominant proteins. Later, biochemical techniques have been used such as isoenzyme analysis. The main goal of these methods is to provide a simple, rapid and reproducible techniques to differentiate Trichinella parasites. For this purpose DNA-based methods appeared the best ones. Beginning with the use restriction enzymes, repetitive DNA probes for detection of parasite DNA, and later techniques based on the polymerase chain reaction (PCR), give results at the high level of sensitivity. All of this information has been used to construct a new taxonomy of the genus Thrichinella. To date, 11 taxa have been recognized in the genus: 8 species (Trichinella spiralis T1, Trichinella nativa T2, Trichinella britovi T3, Trichinella pseudospiralis T4, Trichinella murrelli T5, Trichinella nelsoni T7, Trichinella papuae T10, Trichinella zimbabwensis T11) and additionally three genotypes whose taxonomic status is yet uncertain (T6, T8, T9). Based upon morphology, epidemiology of trichinellosis, geographical distribution and host range of the parasite, two main groups are recognized in the genus Trichinella. The first group comprises species that encapsulate in host muscle tissue, while the species of the second group do not encapsulate. The species and genotypes of the first group infect only mammals (T. spiralis, T. nativa, T. britovi, T. murrelli, T. nelsoni, T6, T8 and T9), whereas of the three species from the second group, one parasitises mammals and birds (T. pseudospiralis) and the other two infect mammals and reptiles (T. papuae and T. zimbabwensis). Due to the big genetic differences between Trichinella isolates, investigators predict that the number of species and genotypes found within Trichinella will be increased.
    Źródło:
    Annals of Parasitology; 2006, 52, 3; 165-173
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Metody różnicowania gatunków nicieni z rodzaju Trichinella
    Methods and tools for parasite differentiation within the genus Trichinella
    Autorzy:
    Pastusiak, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/2144338.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    izoenzymy
    hybrydyzacja kwasow nukleinowych
    roznicowanie gatunkow
    pasozyty
    nicienie
    sekwencje DNA
    Warszawa konferencja
    DNA mitochondrialny
    parazytologia
    konferencje
    Trichinella
    zroznicowanie gatunkowe
    lancuchowa reakcja polimerazy
    biologia molekularna
    znakowanie DNA
    Opis:
    This review summarizes the major biological, biochemical and molecular methods which have been developed during last 20 years to distinguish parasites of the genus Trichinella. From the time of the discovery of Trichinella in 1835 until the 1970, it was assumed that trichinellosis was caused by a single species of parasite, Trichinella spiralis. Many biological parameters have been compared to differentiate the parasite, such as host specificity, geographical distribution, reproductive abilities, nurse cell development and resistance to freezing. Now, investigators realize that the genus Trichinella is a much more complex group of parasites and simple biological methods are unsufficient. In order to identify and better characterize the species and genotypes of Trichinella it was necessary to develop more sensitive techniques. First, for detecting Trichinella infection immunological methods have been used, such as detection of antibodies in host blood and antigens of parasites using monoclonal antibodies against immunodominant proteins. Later, biochemical techniques have been used such as isoenzyme analysis. The main goal of these methods is to provide a simple, rapid and reproducible techniques to differentiate Trichinella parasites. For this purpose DNA-based methods appeared the best ones. Beginning with the use restriction enzymes, repetitive DNA probes for detection of parasite DNA, and later techniques based on the polymerase chain reaction (PCR), give results at the high level of sensitivity. All of this information has been used to construct a new taxonomy of the genus Thrichinella. To date, 11 taxa have been recognized in the genus: 8 species (Trichinella spiralis T1, Trichinella nativa T2, Trichinella britovi T3, Trichinella pseudospiralis T4, Trichinella murrelli T5, Trichinella nelsoni T7, Trichinella papuae T10, Trichinella zimbabwensis T11) and additionally three genotypes whose taxonomic status is yet uncertain (T6, T8, T9). Based upon morphology, epidemiology of trichinellosis, geographical distribution and host range of the parasite, two main groups are recognized in the genus Trichinella. The first group comprises species that encapsulate in host muscle tissue, while the species of the second group do not encapsulate. The species and genotypes of the first group infect only mammals (T. spiralis, T. nativa, T. britovi, T. murrelli, T. nelsoni, T6, T8 and T9), whereas of the three species from the second group, one parasitises mammals and birds (T. pseudospiralis) and the other two infect mammals and reptiles (T. papuae and T. zimbabwensis). Due to the big genetic differences between Trichinella isolates, investigators predict that the number of species and genotypes found within Trichinella will be increased.
    Źródło:
    Wiadomości Parazytologiczne; 2006, 52, 3; 165-173
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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