- Tytuł:
-
Analiza antygenow somatycznych nicienia Aspiculuris tetraptera [Oxyuridae] oraz ich rola w tworzeniu odpowiedzi immunologicznej u myszy laboratoryjnych
The analysis of somatic antigens extracted from Aspiculuris tetraptera [Oxyuridae] and their role in eliciting immune response in laboratory mice - Autorzy:
-
Zalesny, G
Perec-Matysiak, A.
Okulewicz, A. - Powiązania:
- https://bibliotekanauki.pl/articles/840712.pdf
- Data publikacji:
- 2006
- Wydawca:
- Polskie Towarzystwo Parazytologiczne
- Tematy:
-
odpowiedz immunologiczna
Aspiculuris tetraptera
antygeny somatyczne
Oxyuridae
parazytologia
nicienie
pasozyty
myszy laboratoryjne - Opis:
- Background. The aim of this study was isolation and examination of Aspiculuris tetraptera somatic proteins and somatic antigens role in eliciting of immune response in laboratory mice. Material and methods. In our investigation 40 laboratory mice (BALB/c strain) were used. To extract the somatic proteins Tris-HCl buffer with NaCl and Triton X-100 was used. The analysis of somatic antigens was undertaken by Western blotting. Results. The study showed the presence of 14 protein bands ranging from ~ 82 to 28 kDa. Glicoproteins detection revealed 13 bands in range between ~ 70 to 30 kDa. There was no reaction observed with immunoglobulins IgA. Comparision of these results with earlier studies concerning S. obvelata somatic antigens show that there are proteins and glicoproteins with the same molecular weights for both species. It is also observed that S. obvelata somatic extract is more diversed and have higher antigenicity than A. tetraptera. Hence, we may suppose this fact could favour easier colonization of the host by A. tetraptera.
- Źródło:
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Annals of Parasitology; 2006, 52, 2; 109-113
0043-5163 - Pojawia się w:
- Annals of Parasitology
- Dostawca treści:
- Biblioteka Nauki
Artykuł